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Vol. 1 / No. 1 / Published : 2008-01

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Original Article :

Identification of excretion – secretion protein profileof the adult haemonchus contortus with sds-page

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      Abstract :

      The aim of this research is to identify excretion – secretion protein (ESP) profile of Haemonchus contortus which presented in mollecular weight. One hundred female Haemonchus contortus were isolated from sheeps and goats’ abomasum from Surabaya Slaughter House, worms were washed by Phosphat Buffer Saline (PBS) then incubated in PBS with pH 7,0 and temperature 37°C for a night. Excretion – secretion liquid that worms produced in PBS isolated with saturated ammonium, then SDS-PAGE (Sodium Dodecyl Sulphate-Polyacrylamid Gel Electrophorese) method used to identified excretion – secretion protein profile. Result of this research got 5 excretion – secretion protein profile : 42,3 kilo Dalton (kDa); 39,3 kDa; 28,9 kDa; 24,4 kDa and 13,9 kDa.

      Keyword :

      Haemonchus contortus, ESP, SDS-PAGE

      References :


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