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Jurnal Agro Veteriner

ISSN 2303-1697

Vol. 1 / No. 0 / Published : 0000-00

TOC : 9, and page :99 - 109

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Original Article :

Isolation and identification of staphylococcus aureus from raw milk resistant against non β-lactam antibiotics

Author :

  1. Aulia Pradipta*1
  2. Romziah Sidik*2
  3. Mustofa Helmi Effendi*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this research was to study the resistance of Staphylococcus aureus isolated from raw milk in 5 dairy farms in Surabaya against non β-lactam antibiotics. Fourthy samples were culture on Mannitol Salt Agar from which 23 samples were identified there were Staphylococcus sp and from it were identified 7 (30,44%) samples were Staphylococcus aureus by showed characteristic such as mannitol fermented, grape/cluster shape, Gram positive, catalase positive, coagulate positive and had β haemolysin on Blood Agar. Antibiotic disk that used for sensitivity test were Tetracycline, Erythromycin, and Gentamicin using Kirby-Bauer Method. The results showed that the precence of Tetracycline resistance in a sample from K and P dairy farm and the presence of Erythromycin resistance in a sample from K and W dairy farm. The existence of this resistance causes milk dangerous to be consumed by public health because the resistant bacteria, or genetic determinants of resistance, can be transmitted from animals to humans via foodstuffs and direct contact with animals.

Keyword :

Staphylococcus aureus, raw milk, antibiotic sensitivity test, non β-lactam, antibiotics,


References :

  1. Effendi, H.M., Kuntaman and A.T. Soelih. E., (2005). Isolasi dan Identifikasi Gen Penyandi Protein A Sebagai Faktor Virulensi Dari Staphylococcus aureus Pada Kasus Mastitis.. Surabaya : Lembaga Penelitian Dan Pengabdian Kepada Masyarakat Universitas Airlangga. Fakultas Kedokteran Hewan. Universitas Airlangga.
  2. Pieshesa, D., (2011). Resistensi Staphylococcus Koagulase Positif Terhadap Antibiotik Non Beta Laktam Isolat Dari Kasus Pyoderma Pada Anjing [Skripsi].. Surabaya : Fakultas Kedokteran Hewan. Universitas Airlangga.


   


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