Berkala Ilmiah Kimia Farmasi
ISSN 2302-8270
Vol. 5 / No. 2 / Published : 2016-11
Order : 2, and page :8 - 10
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Original Article :
Inhibitory activity of probiotic fermented milk against eschericia coli atcc 6538 and staphylococcus aureus atcc 8739
Author :
- Ramayana*1
- Mahasiswa Fakultas Farmasi
Abstract :
Tujuan dari penelitian ini adalah untuk mengevaluasi susu fermentasi probiotik Lactobacillus acidophilus (L.a) dan aktivitas penghambatan Lactobacillus casei (L.c) terhadap Eschericia coli dan Staphylococcus aureus. Susu fermentasi disiapkan dengan menginokulasi setiap probiotik dalam susu segar yang dipasteurisasi pada suhu 43ºC dan kemudian diinkubasi pada suhu 37oC selama 24 jam. Metode difusi agar dengan menggunakan media nutrient agar digunakan untuk menilai konsentrasi penghambatan minimum (MIC). Hasil penelitian menunjukkan bahwa pH susu probiotik fermentasi mencapai 5. Diameter zona hambat susu fermentasi L. acidophilus menunjukkan penghambatan optimal terhadap Eschericia coli dan Staphylococcus aureus dengan nilai MIC masing-masing sebanyak 14 mm. Diameter zona hambat susu fermentasi L.casei menunjukkan populasi yang optimal terhadap Eschericia coli dan Staphylococcus aureus dengan masing-masing MIC masing-masing sebanyak 12 mm dan 13 mm. Susu fermentasi L. acidophilus probiotik memiliki aktivitas penghambatan yang kuat terhadap Eschericia coli dan Staphylococcus aureus daripada susu probiotik Lactobacillus casei.
Keyword :
Aktivitas hambat, Lactobacillus acidophilus, Lactobacillus casei, Eschericia coli, Staphylococcus aureus,
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 Volume : 5 / No. : 2 / Pub. : 2016-11 |
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