UNIVERSITAS AIRLANGGA



Detail Article

Berkala Ilmiah Kimia Farmasi

ISSN 2302-8270

Vol. 3 / No. 1 / Published : 2014-06

Order : 5, and page :28 - 35

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Original Article :

Pengaruh ph eluen terhadap hasil desalting crude pertussis toxin (pt) dan filamentous hemagglutinin (fha) dari bordetella pertussis

Author :

  1. Isnaeni*1
  2. Esti Hendradi*2
  3. Neni Nurainy*3
  4. Faris Adrianto*4
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Staff PT. Biofarma
  4. Dosen Fakultas Farmasi

Abstract :

Optimizing desalting crude process of PT and FHA using sephadex G-25 as intermediate purification phase for PT and FHA as acellular pertussis vaccine components has been developed. The factor influencing optimization including the impact of chemical factor such as eluation buffer pH towards protein’s concentrate were studied using 3 different pH (6, 7, and 8).  The result were : Different Buffer pH has influence towards protein recoveries whereas phosphate buffer 50 mM pH 7.0 as eluation buffer (with 74,23 + 3,07 % recoveries percentage) best for desalting crude PT and FHA.     

Keyword :

Desalting, Crude PT-FHA, Sephadex G-25, buffer pH, buffer concentration,


References :

Sato Y., Cowell JI., Sato H. Burstin D.G., Manclark C.R.,,(1993) Separation and purification of the hemagglutinin from Bordetella pertussis. Journal : Infect Immun 41(1)





Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2014-06
  1. Penentuan Kemurnian Adenovirus Menggunakan Kromatografi Cair Kinerja Tinggi
  2. Validasi Metode Headspace-gas Chromatography-mass Spectrometry Untuk Analisis Multiresidu Pestisida Organofosfat Pada Sayuran
  3. Penggunaan Zat Pendestruksi Pada Cara Wet Ashing Untuk Analisis Timbal Dalam Sari Buah Kaleng Dengan Metode Spektroskopi Absorpsi Atom
  4. Aktivitas Antimikroba Dan Analisis Klt-densitometri Metabolit Fraksi –fraksi Ekstrak Endofit Dari Aglaia Odorata
  5. Pengaruh Ph Eluen Terhadap Hasil Desalting Crude Pertussis Toxin (pt) Dan Filamentous Hemagglutinin (fha) Dari Bordetella Pertussis
  6. Daya Hambat Kombinasi Susu Probiotik (lactobacillus Acidophilus + Lactobacillus Bulgaricus) Dan Pasta Tomat Terhadap Escherichia Coli Dan Staphylococcus Aureus
  7. Prospektif Kombinasi Susu Probiotik Lactobacillus Acidophilus Dan Bifidobacterium Bifidum Sebagai Sediaan Anti Diare

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