Catalog :




Detail Article

Berkala Ilmiah Kimia Farmasi

ISSN 2302-8270

Vol. 4 / No. 1 / Published : 2015-06

TOC : 4, and page :21 - 28

Related with : Scholar   Yahoo!   Bing

Original Article :

Profil bioautogram bakteriosin dalam sediaan susu probiotik

Author :

  1. Isnaeni*1
  2. A. Toto Poernomo*2
  3. Floriana Nataly*3
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Dosen Fakultas Farmasi

Abstract :

Probiotic milk is a milk that contain probiotic bacteria. Probiotics is life microbial feed supplement; which beneficially affect the host animal by improving its intestinal microbial balance. The probiotic bacteria produce antimicrobial substances; that can inhibit the growth of pathogenic bacteria, that called bacteriocin. One of the bacteriosin is nisin, which exhibit antimicrobial activity against a wide range of Gram positive bacteria, and is particularly affective against bacterial spores. Seven from twenty probiotic milk samples contained in the marketplace, have been extracted to precipitate the bacteriocin. The extracts were then analyzed by using TLC-bioautography methods with n-buthanol, acetat acid, water (4:1:1) and Staphylococcus aureus ATCC 25923 as pathogenic bacteria. Of inhibition zone diameter obtained, can be known whether in the same concentration, samples of probiotic milk have different antibacterial activity. There are different profile bioautogram from extracted samples of probiotic milk. Three from seven extracted samples have the same rf, but none of that extracted samples have the same rf with nisin. All of extracted sample have microbial activity but smaller than nisin activity to against Staphylococcus aureus ATCC 25923 bacteria.      

Keyword :

probiotic milk, bioautography, bacteriocin, nisin, Staphylococcus aureus.,


References :

  1. Cleveland, J., Montville, T. J., Nes, I. F. & Chikindas, M. L, (2001). Bacteriosin:safe, natural antimicrobials for food preservation. Journal : Int. J. Food Microbiol. 71


   


Archive Article

Cover Media Content

Volume : 4 / No. : 1 / Pub. : 2015-06
  1. Pengaruh pasta tomat (lycopersicon esculentum mill.) pada daya hambat lactobacillus acidophilus fncc-0051 dan lactobacillus plantarum fncc-0027 terhadap candida albicans
  2. Sintesis n’-benzilidenbenzohidrazida dan n’-(4-metoksibenziliden)-benzohidrazida dengan material awal metilbenzoat
  3. Validasi metode ekstraksi quechers untuk analisis multiresidu pestisida pada wortel secara kromatografi gas-spektrometri massa
  4. Profil bioautogram bakteriosin dalam sediaan susu probiotik
  5. Aktifitas antimikroba fraksi-fraksi dari ekstrak etil asetat jamur endofit yang diisolasi dari aglaia odorata lour.
  6. Modifikasi struktur dan prediksi aktivitas antikanker senyawa baru turunan aliltiourea secara in silico
  7. Penapisan aktivitas antioksidan kombinasi yogurt dan jus tomat dibandingkan vitamin c