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Detail Article

Berkala Ilmiah Kimia Farmasi

ISSN 2302-8270

Vol. 3 / No. 2 / Published : 2014-11

TOC : 4, and page :23 - 30

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Original Article :

Purifikasi parsial enzim fibrinolitik tempe kacang koro (canavalia ensiformis) produk fermentasi rhizopus oryzae fncc 6078

Author :

  1. A. Toto Poernomo*1
  2. Sudjarwo*2
  3. Raissa Ardinta Parasati*3
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Mahasiswa Fakultas Farmasi

Abstract :

In this work, tempeh of Canavalia ensiformis that fermented by Rhizopus oryzae FNCC 6078 was used as a source of fibrinolytic enzyme. This enzyme could provide clinical application for blood clot removal. That fibrinolytic enzyme was extracted by blending and liofilization. The crude enzyme extract was assaying in fibrin plate agar showing fibrinolytic activity. Partial purification of the crude enzyme extract was performed by two steps, i.e., by ammonium sulphate fractionation followed by gel filtration chromatography using sephadex G-100 as stationary phase. The fibrinolytic activity were evaluated at each step of purification. Fibrinolytic enzyme obtained from 50-70% ammonium sulfate fraction had the highest spesific activity 6,6 x 10-2 U/mg; 10,0 x 10-2 U/mg; dan 23,8 x 10-2 U/mg. There was no significant difference between spesific activity of sample before and after partial purification.      

Keyword :

Chromatography filtration, Partial purification, Rhizopus oryzae, Canavalia ensiformis Tempe,


References :

  1. Sugimoto, S., Fuji, T., Morimiya, T., Johdo, O., Nakamura, T, (2007). The Fibrinolytic Activity of a Novel Protease Derived from Tempeh Producing Fungus, Fusarium sp. BLB. Journal : Biosci Biotechnol Biochem., 71


   


Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2014-11
  1. Pembuatan pereaksi cepat untuk deteksi dini cemaran logam berat cadmium
  2. Docking molekul dan sintesis turunan asam benzoil salisilat tersubstitusi klor sebagai penghambat siklooksigenase-2
  3. Aktivitas antibakteri kombinasi probiotik (bifidobacterium bifidum dan lactobacillus acidophilus) dengan infus daun jambu biji (psidium guajava)
  4. Purifikasi parsial enzim fibrinolitik tempe kacang koro (canavalia ensiformis) produk fermentasi rhizopus oryzae fncc 6078
  5. Validasi metode spektrofotometri-vis pada penetapan kadar boraks di dalam bakso
  6. Upaya untuk menghasilkan 1-(4-bromobenzoyloxy)urea sebagai calon obat antikanker
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