UNIVERSITAS AIRLANGGA



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BIOSAINS

ISSN 1412-1433

Vol. 14 / No. 3 / Published : 2011-09

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Original Article :

Analysis of blood product trombocyte concentrate in red cross blood donor unit surabaya

Author :

  1. Nur Achmad Tjiptoprajitno*1
  2. Aryati*2
  3. I Ketut Sudiana*3
  1. Stikes Satria Bhakti Nganjuk (Mantan Direktur Unit Transfusi Darah PMI Surabaya)
  2. Fakultas Kedokteran Universitas Airlangga
  3. Fakultas Kedokteran Universitas Airlangga

Abstract :

Bacterial contamination in blood produts, especially Thrombocyte Consentrate (TC) still remains a problem in the world with potentialclinical consequences from mild symptom to death. Presently, Blood Banks all over the world keep their TC product in careful mannersince even extremely small numbes of bacteria can multiply to vast under storage at 22–20° C and dangerous levels during theirstorage period. In addition, the demands on TC were increasing, some of which were used mostly for immunosuppressive patients aswell as for multiple transfusion. TC product is also called an indicator for other products such as Whole Blood (WB), Pack Red Cell(PRC) etc. In Indonesia, the reports of bacterial contaminations are minimal. The objective of this study was to discover the possibilityof bacterial contamination in TC product at Red Cross Blood Donor Unit (BDU) in Surabaya. BacT/ALLERT equipment was usedto culture and detect the samples for aerobic and anaerobic bacteria. Therefore, whenever the bacterial contamination on TC turnedpositive, precautions were taken during the next manufacturing process. This is due to the risk contained in TC product as well as itsrole as an indicator of other products. WB products and donor blood were also cultured and served as a comparison and to analyzeany source in which possible contaminations take place. The species of detected bacteria was identified using VITEK-2, which waspreviously examined for Gram positive and negative bacteria using Direct Smear. The result of this study showed 3 samples of TCpositively identified as Staphylococcus hominis and Propionibacterium acne where as WB product showed no contamination. Foursamples of TC donor blood were positively identified as Sphingomonas paucibilis and Propionibacterium acne and 4 samples of WBdonor blood were positively identified as Prophymonas assaccharolitica and Fusobacterium varium. The analysis of this study concludedthat bacterial contamination can occur in platelet products with prevalence rates 5%, and 6.67% in blood donors where as in WBproduct there were no contamination found. The bacterial contaminations in donor blood were not followed by the contaminationsin blood products derived from the same donor.The species of bacteria were commensal and less or non pathogenic However, thepresence of the non pathogenic bacteria is a subject as a precaution.The critical point from which contamination takes place includethe manufacturing of TC, phlebotomy process and probable subclinical bacteremia donor.

Keyword :

TC blood product, , bacterial contamination, , bacterial detection and identification, , precaution on TC production,


References :

Barker LM, Nanassy OZ, Reed MW, Geelhood SJ, Pfalzgraf RD, Cangelosi GA, de Korte D.,(2010) Multiple pH measurement during storage may detect bacterially contaminated platelet concentrates. - : Transfusion

Blajchman MA,(2004) Bacterial contamination of celluler blood component: risk, source and control - : Vox Saguinis

Brecher ME and Hay SN,(2005) Bacterial Contamination of Blood Components - : Clinical Microbiology Review,





Archive Article

Cover Media Content

Volume : 14 / No. : 3 / Pub. : 2012-09
  1. The Effects Of Moderate Intensity Walking Exercise On Stem Cell Mobilization, Diferentiation And Maturation In Pbmc And Bone Remodeling Of Postmenopausal Women
  2. Identification Of Fertilization Receptor Protein (zp3) On Goat Zona Pellucida As Candidate Of Immunocontraceptive Substance Using In Vitro Fertilization Technique
  3. Osteoclast Increasing Number In Periapical Inflammation Due To Lipopolisaccharide Porphyromonas Gingivalis Induction
  4. Analysis Of Blood Product Trombocyte Concentrate In Red Cross Blood Donor Unit Surabaya
  5. Biofilm Formation By Streptococcus Uberis Associated With Chronic Intramammary Infections
  6. Antioxidant Potential Of The Extract Of Green Tea (camellia Sinensis) And Red Tea (hibiscus Sabdariffa) In Atherogenesis Process In Rats With Atherogenic Diet
  7. Seperation Of Bearing X And Y Chromosome Limosin Bull Spermatozoa By Using Percoll And Chicken Eggs Albumen

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