UNIVERSITAS AIRLANGGA



Detail Article

BIOSAINS

ISSN 1412-1433

Vol. 14 / No. 3 / Published : 2012-09

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Original Article :

Biofilm formation by streptococcus uberis associated with chronic intramammary infections

Author :

  1. Vincentius Mubiarto Setiawan*1
  2. Soelih Estoepangestie*2
  3. Setiawan Koesdarto*3
  1. Kesehatan Hewan Laboratorium PT Greenfields Indonesia
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

Mastitis is an inflammation of the mammary gland and is extremely problematic in the dairy industry. Streptococcus uberis is one ofthe main infectious agents responsible for causing mastitis. We speculate that one of the reasons behind the prevalence of S. uberisinfections is its ability to form biofilms. The objectives of this study were to determine in vitro slime production, biofilm formationand the presence of extracelluler matrix associated with biofilm production. Twenty four strains of S. uberis isolated from the chronicmastitic cows and were tested by microtiter plate (MTP) and Scanning Electron Microscopy (SEM). Staphylococcus epidermidis ATCC12228, was used as a negative control Staphylococcus aureus ATCC 25923 and Staphylococcus simulans ATCC 27851 was used aspositive control. All 12 strain also displayed a biofilm-phenotype when tested by MP. The strains were further classified as weak (69%)and strong (31%) biofilm formers. The Streptococcus uberis strain that show biofilm formation ability was showing the presence ofextracelluler matrix associated with biofilm production on SEM. In summary, S. uberis is capable of forming biofilms in vitro on anabiotic surface. The economic impact of Understanding the virulence factors that influence S. uberis’s ability to colonize and maintaininfections will ultimately allow for effective and appropriate treatment protocols that could greatly decrease the impact of mastitiscaused by this pathogen in the dairy industry.

Keyword :

Streptococcus uberis, , biofilm, , chronic mastitis,


References :

Atshan SS, MN Shamsudin, LTT Lung, Z Sekawi, E Ghaznavi-Rad, and CP Pei,(2012) Comparasion of Genotypically Different Clones of MRSA in the Production of Biofilm. - : J. of Biomed. And Biotech.

Davey ME, and G O’Toole,(2000) Microbial Biofilm: from Ecology to Molecular Genetics. - : Mic and Mol.

Gonzalez RN, DE Jasper, NC Kronlund, TB Fayerr, JS Cullor, RB Bushnell, and JD Dellinger,(1990) Clinical Mastitis in Two California Dairy Herds Participating n Contagious Mastitis Control Programs. - : J Dai. Sci.





Archive Article

Cover Media Content

Volume : 14 / No. : 3 / Pub. : 2012-09
  1. The Effects Of Moderate Intensity Walking Exercise On Stem Cell Mobilization, Diferentiation And Maturation In Pbmc And Bone Remodeling Of Postmenopausal Women
  2. Identification Of Fertilization Receptor Protein (zp3) On Goat Zona Pellucida As Candidate Of Immunocontraceptive Substance Using In Vitro Fertilization Technique
  3. Osteoclast Increasing Number In Periapical Inflammation Due To Lipopolisaccharide Porphyromonas Gingivalis Induction
  4. Analysis Of Blood Product Trombocyte Concentrate In Red Cross Blood Donor Unit Surabaya
  5. Biofilm Formation By Streptococcus Uberis Associated With Chronic Intramammary Infections
  6. Antioxidant Potential Of The Extract Of Green Tea (camellia Sinensis) And Red Tea (hibiscus Sabdariffa) In Atherogenesis Process In Rats With Atherogenic Diet
  7. Seperation Of Bearing X And Y Chromosome Limosin Bull Spermatozoa By Using Percoll And Chicken Eggs Albumen

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