UNIVERSITAS AIRLANGGA



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BIOSAINS

ISSN 1412-1433

Vol. 13 / No. 2 / Published : 2011-05

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Original Article :

Effective growth medium, light intensity and length of radiation for culturing isolated microcystis from sutami reservoir in laboratory

Author :

  1. Catur Retnaningdyah*1
  2. Umi Marwati*2
  3. Agoes Soegianto*3
  4. Bambang Irawan*4
  1. Jurusan Biologi Fakultas MIPA Universitas Brawijaya
  2. Departemen Biologi, Fakultas Sains dan Teknologi, Universitas Airlangga
  3. Departemen Biologi, Fakultas Sains dan Teknologi, Universitas Airlangga
  4. Departemen Biologi, Fakultas Sains dan Teknologi, Universitas Airlangga

Abstract :

The main objective of this research was to determine the appropriate method, in terms of media type, light intensity and length of radiation, for culturing Microcystis in laboratory. The treatments were natural media by filtering and sterilizing the water from Sutami reservoir and four variations of selective media, i.e. ASN III, BG11, Bold 3 N, and B12. The research on the influence of light intensity and length of radiation used BG 11 media with varied light intensity (2–3, 5–6 and 8–9 KLux) and length of radiation (12 and 24 hours per day). A control treatment was also conducted by using incubation in a greenhouse with naturally light intensity and radiation. Microcystis abundance was calculated every day. This abundance variable was used to calculate the rate of growth (β) and carrying capacity (γ) of Microcystis, which can be supported by each media treatment. The research result showed that the effective medium for culturing Microcystis in the laboratory was selective medium of B12 and natural medium obtained from Sutami reservoir which had been filtered and sterilized. The highest carrying capacity of Microcystis was found at 8-9 KLux light intensity treatment with radiation duration of 12 hours per day. However, the optimal growth rate of Microcystis was found in the culture at 2–9 KLux light intensity and 24 hours per day radiation duration.

Keyword :

Microcystis, , growth medium, , light intensity, , length of radiation,


References :

A tlas RM,,(2004) Handbook of Microbiological Media. Third Edition. New York. : CRC Press Inc.,

Closs G, Downes B, Boulton A,(2006) A Scientific Introduction Freshwater Ecology Malden USA : Blackwell Publishing

Clesceri LS, Greenberg AE, Eaton AD,(1998) Standard Methods for the Examination of Water and Waste Water Washington. : 20th Ed

Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams STST,(2002) Bergey’s Manual of Determinative Bacteriology. Baltimore : Lippincott Williams and Wilkin

Isaac S and Jennings D,(1995) Microbial Culture. - (eds.) U.K. : Oxford





Archive Article

Cover Media Content

Volume : 13 / No. : 2 / Pub. : 2011-05
  1. Antimalarial Activity And Mechanism Of Action Of Flavonoid Compounds Isolated From Artocarpus Champeden Spreng Stembark
  2. Studi On The Espression Of Tgf-b1 Mmp-9, Collagen Type-i, Collagen Tipe-iv, Glomerulosclerosis, Interstisial Fibrosis, And Albuminuria In Hephrotoxic Docorubin And Nephroprotective Pentoxifyllin Events On Male Swiss-strain Mice
  3. Characterization Of Physical Properties From Human Enamel Induced By Nd:yag Pulsed Laser Based On Laser Speckle Imaging System
  4. Protein Expression Of Cox-2 In High And Low Response In Nasopharynx Carcinoma After Radiotherapy
  5. Gene Polymorphism Of Mthfr C677t And Ts 5’-utr 3r/3r, On Childhood Acute Lymphoblastic Leukemia Resistance To Methotrexate
  6. Effective Growth Medium, Light Intensity And Length Of Radiation For Culturing Isolated Microcystis From Sutami Reservoir In Laboratory
  7. Nutrition And Iron Status In Children With Tb Parents, Their Role On Infection And Disease

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