UNIVERSITAS AIRLANGGA



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BIOSAINS

ISSN 1412-1433

Vol. 14 / No. 3 / Published : 2012-09

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Original Article :

Osteoclast increasing number in periapical inflammation due to lipopolisaccharide porphyromonas gingivalis induction

Author :

  1. Ade Ratri Yustina*1
  2. Ketut Suardita*2
  3. Dian Agustin W*3
  1. Mahasiswa Kedokteran Gigi Fakultas Kedokteran Gigi Universitas Airlangga
  2. Departemen Konservatif Fakultas Kedokteran Gigi Universitas Airlangga
  3. Departemen Konservatif Fakultas Kedokteran Gigi Universitas Airlangga

Abstract :

The pulp chamber is mostly infected by anaerob gram negative bacteria and one of them is Porphyromonas gingivalis. The cell wall ofthis bacteria containing Lipopolysaccharide (LPS). LPS can penetrate to the periradicular tissue, act as endotoxin in host and causeperiradicular inflammation that leading to bone destruction. Bone destruction occur is due to the inflammation process that mediatedby immune system. This inflammation is mediated by immune system that can be found the cytokin pro-inflammatory infiltrated in thecell has an important role in bone destruction is known as osteoclast. Osteoclasts are multinucleated, terminally differentiated cellswhich play an essential role in bone resorption. Binding RANK with RANKL are increasing during the osteoclastogenesis process. Themain purpose of this study was to determine the increasing number of osteoclast in periapical tissue that induced by LPS. This studyused laboratory experimental with the post test only control group design. A total of 21 male rats were divided into 3 groups. GroupA: control group, group B: the teeth were induced by lipopolysaccharide, group C: the teeth only induced by PBS steril. The animalswere sacrificed on 3 weeks after and prepared for histological examination of periradicular tissue response.The analysis revealed thatthe osteoclast increased significantly in group B when lipopolysaccharide was applied in the pulp chamber. The increasing number ofosteoclast is a complex process because of the contribution of TLR4, IL-1 IL6 and TNF-α. There was an increasing number of osteoclastin periapical inflammation that induced by lipopolysaccharide.

Keyword :

Lipopolysaccharide, , periapical, , bone destruction, , osteoclast,


References :

Abbot PV,(2004) Classification, diagnosis, and clinical manifestation of apical periodontitis. - : Endodontic topics. Copyright Blackwell Munksgaard.

Dumitrescu AL,(2006) Histological Comparison of Periodontal Inflammatory Changes in Two Models of Experimetal Periodontitis In The Rat. Bristol : School of Medical Science University Of Bristol

Graves DT, Oates T, Garlet GP,(2011) Review of osteoimmunology and the host response in endodontic and periodontal lesions. - : Journal of microbiologi Oral.





Archive Article

Cover Media Content

Volume : 14 / No. : 3 / Pub. : 2012-09
  1. The Effects Of Moderate Intensity Walking Exercise On Stem Cell Mobilization, Diferentiation And Maturation In Pbmc And Bone Remodeling Of Postmenopausal Women
  2. Identification Of Fertilization Receptor Protein (zp3) On Goat Zona Pellucida As Candidate Of Immunocontraceptive Substance Using In Vitro Fertilization Technique
  3. Osteoclast Increasing Number In Periapical Inflammation Due To Lipopolisaccharide Porphyromonas Gingivalis Induction
  4. Analysis Of Blood Product Trombocyte Concentrate In Red Cross Blood Donor Unit Surabaya
  5. Biofilm Formation By Streptococcus Uberis Associated With Chronic Intramammary Infections
  6. Antioxidant Potential Of The Extract Of Green Tea (camellia Sinensis) And Red Tea (hibiscus Sabdariffa) In Atherogenesis Process In Rats With Atherogenic Diet
  7. Seperation Of Bearing X And Y Chromosome Limosin Bull Spermatozoa By Using Percoll And Chicken Eggs Albumen

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