UNIVERSITAS AIRLANGGA



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ISSN 1412-1433

Vol. 13 / No. 2 / Published : 2011-05

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Original Article :

Protein expression of cox-2 in high and low response in nasopharynx carcinoma after radiotherapy

Author :

  1. Widiastuti*1
  2. Trijono Karmawan Soekarna Prija*2
  3. Juliati Hood Alsagaff*3
  4. Widodo Ario Kentjono*4
  1. Fakultas Kedokteran Universitas Sebelas Maret
  2. Fakultas Kedokteran Universitas Airlangga
  3. Fakultas Kedokteran Universitas Airlangga
  4. Fakultas Kedokteran Universitas Airlangga

Abstract :

The morbidity and mortality of nasopharynx carcinoma (NPC) are still high up until now in our country. Radiotherapy (RT) is the primary choice of therapy, but the results of RT for NPC are not satisfactory. Several studies reported a close relationship between the progression of the cancer cells growth and abnormalities at the molecular level. The aim of this study was to find out the differences of protein expression of COX-2 in high and low response in NPC after radiotherapy. The design was an observational Cross Sectional Analytic study. Specimens were obtained from biopsy of 18 NPC patients on pre- and post- RT with γ-ray, 200 cgy dose radiation per fraction, 5 times/week with no interval to reach a total dose of 6600 cgy during 6–7 weeks, targeted on primary tumor in the nasopharynx and cervical lymph nodes, stated with haematoxylin-Eosin staining and immunohistochemistry examination. Protein expression was semi-quantitatively value, in whish positive expression was noted if a brownish-red colour was found on the tumor cell and expressed as percent per field of view. Tumor response was analyzed based on nasopharynx tumor volume (NTV) changes in cc, as calculated from the result of head CT scan using a D-Com program. Data was analyzed with independent t-test and discriminant analysis. There was no significant difference of mean protein expression of COX-2 in NPC high response compared to low response pre-RT. Before RT there was no difference in the response level of variable, as confirmed with statistical analysis of COX-2(p = 0.883). Mean protein expression of COX-2 between high and low response groups after RT was different significantly (p = 0.007). The discriminant analysis showed that protein expression of COX-2 after RT was distinct variables and those variable was capable of discriminating NPC patients into high or low response group with cut off -1,3 and its accuracy value of 88.9%. The conclusion, there was no difference between protein expression of COX-2 in NPC patient with high and low response before RT. There was a difference in protein expression of COX-2 in NPC patient with high and low response after RT. Protein expression of COX-2 in high response of NPC patient was lower than those in the low response after RT. Thus, the high response mechanism in NPC patient after RT, indicated a decreased protein expression of COX-2. Protein expression of COX-2 was able to differentiated high and low response group in NPC patient after RT with cut off -1.3 and accuracy value of 88.9%. Protein expression of COX-2 low response after RT, could differentiate partly and no response groups in NPC patient after RT with cut off value of - 0.268 and different significant of accuracy value of 60%. Protein expression of COX-2 low response after RT could differentiate no response and progressive group in NPC patient after RT with cut off value of 0.184 and different significant of accuracy value of 60%.

Keyword :

radiotherapy, , nasopharyngeal carcinoma, , COX-2, , tumor response,


References :

Abbas AK, Lichtman AH, Pillai S,(2007) ,Immunity to Tumors. In Abbas AK, Lichtman AH, Pillai S (eds): Cellular and Molecular Immunology, 6nd edition. Philadelpia : W.B. Saunders Co

Baert AL,(2008) Apoptosis. In: Encyclopedia of Diagnostic Imaging Volume 2. New York : Springer-Verlag Berlin Heidelberg

Bryan N, Mark C,(2001) Nasopharyngeal cancer. In Bailey BJ (ed): Head and neck surgery otolaryngology, volume one Philadelphia : Lippincott Co

Chew CT ,(2003) Risk factors, symptoms and diagnosis of Nasopharryngeal Carcinoma Singapura : World Scientific Publishing Co

Hill RP, Tannock IF,(1998) Introduction Cancer Biology. In Tannock IF, Hill RP (eds): The Basic Science of Oncology third edition. New York : McGraw-Hill Health Professions Division,





Archive Article

Cover Media Content

Volume : 13 / No. : 2 / Pub. : 2011-05
  1. Antimalarial Activity And Mechanism Of Action Of Flavonoid Compounds Isolated From Artocarpus Champeden Spreng Stembark
  2. Studi On The Espression Of Tgf-b1 Mmp-9, Collagen Type-i, Collagen Tipe-iv, Glomerulosclerosis, Interstisial Fibrosis, And Albuminuria In Hephrotoxic Docorubin And Nephroprotective Pentoxifyllin Events On Male Swiss-strain Mice
  3. Characterization Of Physical Properties From Human Enamel Induced By Nd:yag Pulsed Laser Based On Laser Speckle Imaging System
  4. Protein Expression Of Cox-2 In High And Low Response In Nasopharynx Carcinoma After Radiotherapy
  5. Gene Polymorphism Of Mthfr C677t And Ts 5’-utr 3r/3r, On Childhood Acute Lymphoblastic Leukemia Resistance To Methotrexate
  6. Effective Growth Medium, Light Intensity And Length Of Radiation For Culturing Isolated Microcystis From Sutami Reservoir In Laboratory
  7. Nutrition And Iron Status In Children With Tb Parents, Their Role On Infection And Disease

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