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BIOSAINS

ISSN 1412-1433

Vol. 13 / No. 3 / Published : 2011-09

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Original Article :

Regeneration of massive bone defect with bovine hydroxyapatite as scaffold of mesenchymal stem cells

Author :

  1. F erdiansyah*,*1
  2. Djoko Rushadi*, *2
  3. Fedik Abdul Rantam**,*3
  4. Aulani'am****4
  1. Departemen Orthopedi dan Traumatologi RSU Dr. Soetomo Fakultas Kedokteran Universitas Airlangga
  2. Departemen Orthopedi dan Traumatologi RSU Dr. Soetomo Fakultas Kedokteran Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga
  4. Fakultas MIPA Universitas Brawijaya

Abstract :

Massive bone defect caused by various diseases and disorders, until now is still a major problem in the field of Orthopedics and Traumatology, because if not handled properly can cause permanent disability in patients. Massive bone defect reconstruction need big graft size, and the ideal graft must have characteristics of osteoconductive, osteoinductive, and osteogenesis.This study aimed to prove that there will be bone regeneration process take place in massive bone defect which being treated with bovine hydroxyapatite as scaffold of mesenchymal stem cells.This was a post test only control group design study. Experimental unit was male New Zealand white rabbit, which in this research will be underwent cutting of ulna osteoperiosteal as critical sized bone defect, then reconstruction was performed using a bovine hydroxyapatite (control group) and composite of bovine hydroxyapatite with mesenchymal stem cells. After 8 weeks, the reconstructed ulna has been were taken and being analyzed. Analysis results from control and treatment group were: no difference on radiological examination (p = 0.133), histopathological examination showed difference in total matrix formed (p = 0.00), difference in union distal and proximal (p = 0.000 and p = 0.015), matrix in graft also showed to be different (p = 0.000), difference in bone matrix (p = 0.010). Immunohistochemistry examination showed difference in collagen type I and osteocalcin (p = 0.000 and p = 0.000). Correlation was found between collagen type I bone matrix (p = 0.039 dan p = 0.049). Multivariate analysis showed correlation between osteoblast product and massive bone defect regeneration (p = 0.797). The conclusion of this research was regeneration occur in the reconstruction of massive bone defects using a composite graft of bovine hydroxyapatite with mesenchymal stem cells.

Keyword :

Regeneration, , massive bone defect,


References :

  1. A bdurrahman, (2002). Indonesian Tissue Banking: Progress on Tissue Production and Development of Quality Assurance,. seoul : the 9th International Conference on Tissue Banking-Asia Pacific Association of Surgical Tissue Bank.
  2. A rinzeh TL, Peter SJ, Archambault MP, et.al, , (2003). Allogeneic Mesenchymal Stem Cells Regenerate Bone in Critical-Sized Canine Segmental Defect. . - : J Bone Joint Surg.
  3. Bensaid W, Oudina K, Viateau V, et al,, (2005). De Novo Reconstruction of Functional Bone by Tissue Engineering in the Metatarsal Sheep Model. . - : Tissue.Eng
  4. Bakker A, Schrooten J, Van Ceynenbreugel T et al, (2008). Quantitatif Screening of Engineered Implants in a Long Bone Defect Model in Rabbits. - : Tissue.Eng
  5. Bruder SP, Scaduto TS, (2005). Cell Based Strategies for Bone Regeneration from Development Biology to Clinical Therapy. In (Lieberman JR, Friedlander GE, eds). New Jersey : Bone Regeneration and Repair Biology and Clinical Applications Humana Press


   


Archive Article

Cover Media Content

Volume : 13 / No. : 3 / Pub. : 2011-09
  1. The role of tgf-b1 as switching isotype regulator on siga secretion in saliva
  2. Influent of e. coli and leucocytes againts human sperm quality in vitro
  3. Recombinant gen of encoding b-xylosidase from geobacillus thermoleovorans it-08 in phis1525 plasmid
  4. The potency of blue light emitting diode (led) for photoinactivation of staphylococcus aureus bacteria with endogeneous porphyrin
  5. The effect of tomato (lycopersicum pyriforme) juice on the prevention of photoaging of the skin as a result from ultraviolet-b irradiation
  6. Regeneration of massive bone defect with bovine hydroxyapatite as scaffold of mesenchymal stem cells