UNIVERSITAS AIRLANGGA



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BIOSAINS

ISSN 1412-1433

Vol. 13 / No. 2 / Published : 2011-05

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Original Article :

Studi on the espression of tgf-b1 mmp-9, collagen type-i, collagen tipe-iv, glomerulosclerosis, interstisial fibrosis, and albuminuria in hephrotoxic docorubin and nephroprotective pentoxifyllin events on male swiss-strain mice

Author :

  1. Bambang Purwanto*1
  2. A.Guntur Hermawan*2
  3. R. Moh.Yogyantoro*3
  4. Juliati Hood Alsagaff*4
  1. Sub bagian ginjal hipertensi FK UNS/RSUD Dr. Moewardi Surakarta
  2. Departemen Penyakit Jantung Fakultas Kedokteran Universitas Airlangga
  3. ** Departemen Penyakit Jantung Fakultas Kedokteran Universitas Airlangga
  4. Departemen Patologi Anatomi Fakultas Kedokteran Universitas Airlangga

Abstract :

Nowadays the use of doxorubicin (DXR) in the treatment of cancer has increased. This eventually has also increased cancer morbidity since the nephrotoxic effects of DXR still become a problem. Pentoxifyllin (PTX) as an electron-donor material can be nephroprotective; thus, the combination of DXR and PTX would reduce nephrotoxic effects of DXR. The aim of this study was to examine the differences in the expression of TGF-β1, collagen type-I, collagen type-IV, MMP-9, glomerulosclerosis, fibrosis interstitial, and albuminuria in normal, nephrotoxic and nephroprotective events. Forty-eight male Swiss-strain mice were randomly divided into three groups consisting of 16 mice each. The groups were Control group (C) injected with NaCl 0.9%; Treatment group (P) injected with DXR; and Therapy group (T) injected with the combination of DXR and PTX by intraperitoneally respectivelly. The Injections was done once a week for three consecutive weeks. On the 4th and 8th weeks post-treatment, eight mice from each group were sacrificed. Examination of TGF-β1, collagen type-I, collagen type-IV, and MMP-9 expression was conducted by using immunohistochemistry examination with monoclonal antibody. Meanwile, Glomerulosclerosis and fibrosis interstitial examination with a histopathologist was performed by using Verheoff van Giesen staining. Then, the examination of albuminuria was conducted by applying elisa technique with monoclonal antibody of albumin. The data were analyzed with one-way ANOVA statistics. The mice in the treatment group injected with 100% Doxorubicin showed an increase in the expression of TGF-β1, collagen type-I, collagen type-IV, glomerulosclerosis, fibrosis interstitial and albuminuria, yet a decrease in the MMP-9 expression. Meanwhile, the opposite is seen in the group of PTX combined with DXR. Therefore, this study concludes that Doxorubicin and PTX combination can be used to reduce the nephrotoxic effects of DXR.

Keyword :

doxorubicin;, pentoxyfilline; , glomerulosclerosis;, fibrosis interstitial,


References :

Baratawidjaja KG, Iris R,(2009) Imunologi Dasar. Edisi VIII Jakarta : FK Universitas Indonesia

Bawazier LA,(2006) Albuminuri. Buku Ajar Penyakit Dalam: Ed IV Jakarta : Dept. Penyakit Dalam. Fakultas Kedokteran Universitas Indonesia

Belloc F, Dumani P,(1995) Effect of Pentoxifilline on Apoptosis of Cultural Cells. - : J Cardiovase Pharmacal

Blobe GC, Schiemann WP, and Lodish HF,(2000) Role of Transforming Growth Factor ß in Human Disease. - : N Engl J Med

Erlan JF, Desmant,(2001) Transfers of Ferritin-Bound Iron to Andriamicyn. - : FEBS letters





Archive Article

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Volume : 13 / No. : 2 / Pub. : 2011-05
  1. Antimalarial Activity And Mechanism Of Action Of Flavonoid Compounds Isolated From Artocarpus Champeden Spreng Stembark
  2. Studi On The Espression Of Tgf-b1 Mmp-9, Collagen Type-i, Collagen Tipe-iv, Glomerulosclerosis, Interstisial Fibrosis, And Albuminuria In Hephrotoxic Docorubin And Nephroprotective Pentoxifyllin Events On Male Swiss-strain Mice
  3. Characterization Of Physical Properties From Human Enamel Induced By Nd:yag Pulsed Laser Based On Laser Speckle Imaging System
  4. Protein Expression Of Cox-2 In High And Low Response In Nasopharynx Carcinoma After Radiotherapy
  5. Gene Polymorphism Of Mthfr C677t And Ts 5’-utr 3r/3r, On Childhood Acute Lymphoblastic Leukemia Resistance To Methotrexate
  6. Effective Growth Medium, Light Intensity And Length Of Radiation For Culturing Isolated Microcystis From Sutami Reservoir In Laboratory
  7. Nutrition And Iron Status In Children With Tb Parents, Their Role On Infection And Disease

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