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BIOSAINS

ISSN 1412-1433

Vol. 13 / No. 3 / Published : 2011-09

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Original Article :

The potency of blue light emitting diode (led) for photoinactivation of staphylococcus aureus bacteria with endogeneous porphyrin

Author :

  1. S uryani Dyah Astuti*1
  2. Ni’matuzahroh*2
  3. Muhammad Zainuddin*3
  4. Suhariningsih*4
  1. Departemen Fisika Fakultas Sains dan Teknologi Universitas Airlangga
  2. Departemen Biologi Fakultas Sains dan Teknologi Universitas Airlangga
  3. Fakultas Farmasi Universitas Airlangga
  4. Departemen Fisika Fakultas Sains dan Teknologi Universitas Airlangga

Abstract :

Photodynamic Inactivation (PDI) represents a method for bacteria inactivation by using combination of endogenous porphyrin and light photon. The purpose of this study were to determine the instrument performance of LED light source that had been assembled and to recognize the potency of LED irradiating energy dose for photoinactivation of Staphylococcus aureus bacteria ATCC 6538P with endogenous porphyrin. Performance measurement of LED instrument showed that blue LED had emission peak of 430 nm and red 629 nm with 65 nm and 30 nm bandwidth. The analysis of LED irradiating energy dose by anova indicated that interaction of power and irradiating time of blue LED 430 nm decreased the percentage of Staphylococcus aureus colonies. With optimal irradiating of 75 mW/cm2 power density and 30 minute time of irradiating (energy density 135 J/cm2), it yielded the percentage decrease of Staphylococcus aureus colonies as many as 75%. Hence, the blue LED instrument has a high potential for photoinactivation to Staphylococcus aureus bacteria.

Keyword :

photodynamic inactivation, , LED,


References :

  1. Bruce-Micah R, Huttenberger D, Freitag L, Cullun J, Foth H-J, (2009). Pharacokinetic of ALA and h-ALA Induced Porphyrins in the Models Mycobacterium phlei and Mycobacterium. - : Journal of Photochemistry and Photobiology
  2. Coyle, John D, (1991). Introduction to Organic Photochemistry. . London. : John Willey Sons
  3. Grossweiner LI, (2005). The Science of Phototherapy: An Introduction. USA : Springer
  4. Hamblin MR, Hasan T, (2004). Photodynamic therapy: a new antimicrobial approach to infectious disease? . - : J of Photochem & Photobiology
  5. Karu T, (2003). Low Power Laser Therapy CRC Press LLC: . New York : CRC Press LLC


   


Archive Article

Cover Media Content

Volume : 13 / No. : 3 / Pub. : 2011-09
  1. The role of tgf-b1 as switching isotype regulator on siga secretion in saliva
  2. Influent of e. coli and leucocytes againts human sperm quality in vitro
  3. Recombinant gen of encoding b-xylosidase from geobacillus thermoleovorans it-08 in phis1525 plasmid
  4. The potency of blue light emitting diode (led) for photoinactivation of staphylococcus aureus bacteria with endogeneous porphyrin
  5. The effect of tomato (lycopersicum pyriforme) juice on the prevention of photoaging of the skin as a result from ultraviolet-b irradiation
  6. Regeneration of massive bone defect with bovine hydroxyapatite as scaffold of mesenchymal stem cells