Catalog :




Detail Article

BIOSAINS

ISSN 1412-1433

Vol. 13 / No. 3 / Published : 2011-09

Related with : Scholar   Yahoo!   Bing

Original Article :

The role of tgf-b1 as switching isotype regulator on siga secretion in saliva

Author :

  1. Pratiwi Soesilawati*1
  2. Harianto Notopuro*2
  3. Istiati Soehardjo*3
  4. Afaf Baktir*4
  1. Departemen Biologi Oral, Fakultas Kedokteran Gigi, Universitas Airlangga
  2. Fakultas Kedokteran Universitas Airlangga
  3. Departemen Biologi Oral, Fakultas Kedokteran Gigi, Universitas Airlangga
  4. Fakultas Sains dan Teknologi Universitas Airlangga

Abstract :

IgA Isotype switching requires the induction of TGF-β1 to regulate transcriptional activity. Disturbances in the differentiation of B cells can occur because of an increasing or decreasing in expression of TGF-β1. This difference causes the differentiation of B cells disrupted because TGF-β1 play a role in DNA re-arrangement for isotype switching in B cells, causing changes in membrane-bound antibody IgM became membrand-bound antibody IgA. This Study was aimed to identity and coralabe between sIgA with TGF beta. This research was conducted through the 30 samples from the case group with sIgA levels> 300 ng/ml and 30 samples from the control group with sIgA levels < 300 ng/ ml. Gingival tissues obtained during the extraction of decidous teeth. Gingival tissue was inserted into a solution of 10% buffered formalin. Preparation of histological preparations followed by immunohistochemical staining procedure using monoclonal antibody anti-TGF-β1. This result showed that statistical tests of TGF-β1 in the case group contained an average of 26% of the entire field of view of the lamina propria. Average in the control group by 57% of the entire field of view of the lamina propria. Data on the number of TGF-β1 in the case and control groups are normally distributed. The results of the analysis by t test found significant differences in the mean number of TGF-β1 in the case and control groups (p = 0.000). There is a strong correlation between TGF-β1 and sIgA. Decreased of TGF-β1 expression associated with decreased activation in the sIgA isotype switching, and vice versa.

Keyword :

TGF-b1, siga saliva, immunohistochemistry, switching isotype,


References :

  1. Abbas AK, Lichtman AH, (2007). Basic Immunology: Function and disorders of the immune system. . Philadelphia : Elsevier
  2. Abbas AK, Lichtman AH, (2010). Cellular and Molecular Immunology. 6th Ed. Philadelphia : Elsevier
  3. Brandtzaeg P, (2007). Do salivary antibodies reliably reflect both mucosal and systemic iImmunity? In: Annals of the New York Academy of Sciences. - : -
  4. Kindt TJ, Osborne BA, Goldsby RARA, (2006). Kuby Immunology. 6th Ed. . - : WH Freeman & Co. pp
  5. Lehner T, (1996). Imunologi Pada Penyakit Mulut. Terjemahan Farida dan Suryadana. Ed.3.. Jakarta : EGC press.


   


Archive Article

Cover Media Content

Volume : 13 / No. : 3 / Pub. : 2011-09
  1. The role of tgf-b1 as switching isotype regulator on siga secretion in saliva
  2. Influent of e. coli and leucocytes againts human sperm quality in vitro
  3. Recombinant gen of encoding b-xylosidase from geobacillus thermoleovorans it-08 in phis1525 plasmid
  4. The potency of blue light emitting diode (led) for photoinactivation of staphylococcus aureus bacteria with endogeneous porphyrin
  5. The effect of tomato (lycopersicum pyriforme) juice on the prevention of photoaging of the skin as a result from ultraviolet-b irradiation
  6. Regeneration of massive bone defect with bovine hydroxyapatite as scaffold of mesenchymal stem cells