UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 17 / No. 2 / Published : 2010-11

Order : 1, and page :57 - 62

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Original Article :

(oral administration of mycobacterium tuberculosis 38-kilodalton adhesin protein increases macrophages and lymphocytes in intestinal balb/c mice)

Author :

  1. Rahma Triliana*1
  2. Ade A Kartosen2*2
  3. Dianika P Puspitasari2*3
  4. Sri Murwani3*4
  5. Sanarto Santoso3*5
  6. Maimun Z Arthamin1*6
  1. Departemen Patologi Klinik, Fakultas Kedokteran Universitas Brawijaya/RS Saiful Anwar Malang
  2. Mahasiswa Fakultas Kedokteran Universitas Brawijaya
  3. Mahasiswa Fakultas Kedokteran Universitas Brawijaya
  4. Departemen Mikrobiologi, Fakultas Kedokteran Universitas Brawijaya/RS Saiful Anwar Malang
  5. Departemen Mikrobiologi, Fakultas Kedokteran Universitas Brawijaya/RS Saiful Anwar Malang
  6. Departemen Patologi Klinik, Fakultas Kedokteran Universitas Brawijaya/RS Saiful Anwar Malang

Abstract :

Tuberculosis (TB), caused by Mycobacterium tuberculosis (M.tb), is one of the world health problems. Oral vaccination of M.tb has a potential to reduce the risk and complication of TB. The 38-kDa adhesin protein as one of oral TB vaccine candidates has not been proven. This study is aimed to determine M.tb 38-kDa adhesin protein effect on macrophage and lymphocyte numbers in mice intestine after an oral administration. BALB/c mice (n=20), age 6–8 weeks, and were divided into 4 groups: control (K), adjuvant (A), 38-kDa 100μg adhesin protein (P), and combination of 100μg 38-kDa adhesin protein with adjuvant (PA). An oral administration was given at the beginning with 2 boosters every 4 weeks. After 3 days of the second booster, the mice were killed and the intestine was taken and stained with haematoxylin eosin (HE) to measure its macrophages and lymphocytes number. The mean ±2SD were 18.4 (3.71) and 6.09 (0.34), 23.0 (7.78) and 8.86 (1.19), 42.2 (13.63) and 23.49 (3.91), 95.4 (30.11), and 53.57 (13.79) respectively for K, A, P and PA group. The statistical test showed a significant difference among each group revealing the role of M.tb 38-kDa adhesin protein as immunogenic inducing cellular immunity in intestine. In this study, so far it was found that the oral administration of M. tb 38-kDa adhesin protein has an ability to increase macrophage and lymphocyte numbers in the mice intestinal BALB/c.

Keyword :

Mycobacterium tuberculosis, 38-kDa adhesin protein, mice intestinal macrophages and lymphocytes,


References :

Abbas AK, Lichtman AK, Pober,(2006) Celluler and Moleculer Immunology Philadhelpia : WB. Saunders Company





Archive Article

Cover Media Content

Volume : 17 / No. : 2 / Pub. : 2010-11
  1. The Diagnostic Value Of Heart-type Fatty Acid Binding Protein (h-fabp) Rapid Test Related To Cardiac Troponin I In Non St Elevation Myocardial Infarction (nstemi)
  2. The Creatinine Level And Potassium Serum In Patients With And Without Type Ii Diabetic
  3. Procalcitonin For The Differentiation Of Bacterial And Non Bacterial Infection
  4. Diagnosis Of Dengue Virus Infection With Iga Anti Dengue Rapid Tests
  5. The Platelet Aggregation Test As A Predictor Of Clinical Outcome In Acute Infarction Stroke
  6. Association Between Atypical Depolarization On The Cell-dyn 3200 And The Presence Of Plasmodium Spp In Blood In The Dr. Soetomo Hospital Surabaya
  7. The Correlation Between Thrombocyte And Cd4 Count In Hiv/aids Patients
  8. The Chemotherapy Effect In The Activity Of Transaminase Enzymes In Breast Cancer Patients
  9. Pathogenesis And Laboratory Examination Of Primary Myelofibrosis
  10. Star ()-flagged Leukocytosis As Indicator Of Interfering Factor In Automatic Hematology Analyzer
  11. (oral Administration Of Mycobacterium Tuberculosis 38-kilodalton Adhesin Protein Increases Macrophages And Lymphocytes In Intestinal Balb/c Mice)
  12. Diazo Test As A Screening Test Of Typhoid Fever A Practical Approach

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