UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 19 / No. 3 / Published : 2013-01

Order : 10, and page :190 - 196

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Original Article :

(the panleucogating method for lymphocyte cd4 counting in hiv patients)

Author :

  1. Umi S. Intansari*1
  2. Budi Mulyono*2
  3. Usi Sukorini*3
  1. Bagian Patologi Klinik FK-UGM/ RS DR Sardjito
  2. Bagian Patologi Klinik FK-UGM/ RS DR Sardjito
  3. Bagian Patologi Klinik FK-UGM/ RS DR Sardjito

Abstract :

The standard method for absolute lymphocyte CD4 count is by the use of flow cytometric analysis, but due to the high costly matter, a simpler method and less costly fee of PanLeucogating dual platform is needed. The aim of this study is to know how to replace the single platform by PanLeucogating dual platform by determining the agreement between both methods and to test the proportion of patient who have CD4 <200 cell/ul measured by PanLeucogating and single platform method.  A cross sectional study was carried out in the Departement of Clinical Pathology Faculty of Medicine Gadjah Mada University Yogyakarta. The inclusion criteria of the study are HIV/AIDS patients who their CD4 were measured. Absolute count of CD4 was using FACS Calibur measured with both PanLeucogating and single platform method. The bias, correlation, regression and limit of agreement between both methods were analyzed using Bland Altman analysis to decide whether the two methods are interchangeable. The result of the study revealed an excellent correlation between the two methods (r =  0.996 ; y = - 0.906 + 0.0.987). Bland Altman analysis revealed bias  =  5 cells/uL;  standard deviation = 18.7 and Limit of agreement (LOA)  = -31.6 – 40.75. The Mean Percentage Difference (MPD) = 2%; dan %LOA = -7.96 – 12.5%. PanLeucogating method has a good agreement with the single platform method and can be used interchangeable. There was no difference of the proportion of patient who has CD4 <200 cell/ul measured by Panleucogating method and single platform.     Metode baku pengukuran jumlah limfosit CD4 adalah metode single platform menggunakan flow cytometer. Namun, karena tingginya biaya, diperlukan metode pilihan lain dengan PanLeucogating yang cara kerjanya sederhana dan berbiaya lebih murah. Penelitian ini bertujuan untuk mengetahui kesesuaian metode PanLeucogating dengan single platform dan menguji perbedaan proporsi pasien dengan jumlah limfosit CD4<200 sel/uL yang diperiksa dengan kedua metode. Penelitian ini menggunakan rancangan potong lintang yang dilakukan di Bagian Patologi Klinik Fakultas Kedokteran Universitas Gadjah Mada dan RSUP Dr. Sardjito. Patokan kesertaan subjek adalah pasien terinfeksi HIV yang memeriksakan jumlah limfosit CD4. Jumlah limfosit CD4 diukur dengan metode PanLeucogating dan single platform menggunakan FACS Calibur. Analisis Bland Altman dilakukan untuk mengetahui apakah kedua cara tersebut dapat saling menggantikan. Metode PanLeucogating bernasab sangat baik dengan single platform  (r =  0,996 ; y = -0,906 + 0,0,987). Analisis Bland Altman menghasilkan bias  =  5;  simpang baku = 18,7; MPD = 2 ; Limit of agreement (LOA)  = -31,6 – 40,75; dan %LOA = -7,96 – 12,5%. Metode PanLeucogating berkesesuaian baik dengan single platform dalam pengukuran jumlah absolut limfosit CD4. Metode PanLeucogating juga tidak mempengaruhi perbandingan pasien yang diobati berdasarkan jumlah limfosit absolut  < 200 sel/µL.

Keyword :

Lymphocyte CD4 count, PanLeucogating, HIV infection,


References :

Whitby L, Granger V, Storie I, Goodfellow K, Sawle A, Relly JT, Barnett D,(2002) Quality Control of CD4+ T-Lymphocyte Enumeration: Results From the Last 9 Years of The United Kingdom National External Quality Assessment Scheme for Immune Monitoring (1993-2001) USA : Cytometry (Clinical cytometry)

World Health Organization,(2004) Scaling up Antiretroviaral Therapy in Resource-limited Settings; Treatment Guidelines for a Public Health Approach Geneva : World Health Organization

Pattanapanyasat K, Shain H, Noulsri E, Lerdwana S, Thepthai C, Prasertsilpa V,(2005) A Multicenter Evaluation of PanLeucogating Method and the Use of Generic Monoclonal Antibody Reagents for CD4 Enumeration in HIV-infected Patients in Thailand Thailand : Cytometry Part B

Karcher H, Bohning D, Downing R, Mashate S, Harms G,(2006) Comparison of Two Alternative Methods for CD4+ T-cell Determination (coulter manual CD4 count and Cy flow) against Standar Dual Platform Flow Cytometry in Uganda Ugan



Archive Article

Cover Media Content

Volume : 19 / No. : 3 / Pub. : 2013-01
  1. {(active Caspase-3 In Acute Myeloid Leukaemia (aml) And Acute Lymphoblastic Leukaemia (all)}
  2. (principle Modification Of β-glucan Detection From Candida Albicans In Serum)
  3. Apoptosis Index Between Females And Males In Regular Hemodialysis
  4. [iron Deficiency In Pregnant Women By Haemoglobin Reticulocyte (ret-he)]
  5. (higher Level Of Ctx In Osteoporotic Women Compared To Normal And Osteopenic Women)
  6. (cystatin C, Hba1c And Albumin Creatinine Ratio)
  7. (lactate Dehydrogenase (ldh) During Storage)
  8. (cd4+ T Lymphocyte As A Prognosis Predictor In Sepsis Patients)
  9. (angiotensin Ii On Adipocytes Culture Exposed With High Glucose)
  10. (the Panleucogating Method For Lymphocyte Cd4 Counting In Hiv Patients)
  11. (c3c Serum Complement And Blood T-cd4+ Lymphocyte)
  12. (cell Based Hemostatis – In Vivo)
  13. Neonatal Acute Myeloid Leukaemia

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