UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 20 / No. 1 / Published : 2013-01

Order : 2, and page :6 - 11

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Original Article :

(the synergic effect of chloroquine and n-acetyl cysteine in decreasing parasitemia and erythrocyte malondyaldehyde level in balb/c mice infected with plasmodium berghei)

Author :

  1. Agustin Iskandar*1
  2. Sudjari*2
  1. Lab Parasitologi FK UNIBRAW Malang
  2. Lab Parasitologi FK UNIBRAW Malang

Abstract :

Malaria is still a global health problem around the world particularly in Indonesia. Chloroquine is one of the anti-malarial drugs which is still used in Indonesia. The aim of this research is to know the synergic effect of Chloroquine and N-Acetyl Cysteine towards parasitemia and erythrocyte malondyaldehyde levels in mice infected with Plasmodium berghei. This was an experimental research using Plasmodium berghei and BALB/c strain mice, 6-8 weeks old, with body weight 20-30 grams. After infected with Plasmodium berghei, the mice were divided into eight (8) groups: control, chloroquine, NAC 0.25 mg/gBB; NAC 0.5 mg/gBB; NAC 1 mg/gBB and combined drugs of chloroquine and NAC 0.25 mg/gBB; NAC 0.5 mg/gBB; NAC 1 mg/gBB. On the 3rd, 5th, and 7th day after treatment, the mice were killed and their parasitemia and Malondialdehyde (MDA) levels were measured. By using MANOVA and Tukey HSD test, a significant difference in parasitemia level was found between the control and chloroquine group, as well as the control and combined drug groups. In the NAC groups, there was no decrease. The decrease of MDA level began on the 7th day.  It can be concluded that there was a synergic effect between the chloroquine and N-Acetyl Cysteine in decreasing parasitemia and malondyaldehyde levels in mice BALB/c infected with Plasmodium berghei.      Malaria masih merupakan masalah kesehatan utama di dunia. Peningkatan aktivitas radikal bebas berperan sangat penting dalam perjalanan penyakit malaria. Klorokuin masih merupakan pilihan jenis obat dalam pengobatan malaria. N-Acetyl Cysteine selain sebagai antioksidan juga merupakan imunomodulator serta berpeluang kuat sebagai obat anti malaria. Gabungan klorokuin dan N-Acetyl Cysteine diharapkan dapat memberikan berpengaruh sinergi dalam: penurunan derajat parasitemia, penurunan aktivitas radikal bebas selama infeksi Plasmodium berghei. Metode meneliti yang dilakukan adalah dengan penelitian eksperimental yang menggunakan Plasmodium berghei. Hewan uji adalah mencit galur BALB/c umur 6-8 minggu dengan berat badan 20-30 gram. Setelah diinfeksi Plasmodium berghei, mencit dibagi dalam 8 kelompok, yaitu kelompok pembanding, klorokuin, NAC 0,25 mg/gBB, NAC 0,5 mg/gBB dan NAC 1 mg/gBB. Kemudian  kelompok gabungan klorokuin +NAC 0,25mg/gBB dan yang +NAC 0,5 mg/gBB serta yang +NAC 1 mg/gBB. Pada hari ke-3, ke-5 dan ke-7 setelah diberi minum obat, mencit dibunuh dan diperiksa derajat parasitemia, kadar malondialdehyde (MDA) dan glutathion (GSH)-nya. Hasil meneliti diperiksa dengan menggunakan uji MANOVA dan Tukey HSD dan didapatkan penurunan derajat parasitemia yang bermakna. Dan yang paling bermakna menunjukkan penurunan ialah kelompok gabungan klorokuin serta NAC 0,5. Penurunan kadar MDA terdapt pada hari ke-7, sedangkan kadar GSH tercapai pada hari ke-5. Dalam telitian ini dapat disimpulkan bahwa terdapat bukti pengaruh sinergi klorokuin dan N-Acetyl Cysteine dalam penurunan derajat parasitemia dan aktivitas radikal bebas di mencit yang diinfeksi Plasmodium berghei.

Keyword :

Chloroquine, N-acetyl cysteine, parasitemia, malondyaldehyde,


References :

Abbas KA, Lichtman, Prober JS,(2006) Cellular and molecular immunology Philadhelpia : WB. Saunders Company





Archive Article

Cover Media Content

Volume : 20 / No. : 1 / Pub. : 2013-01
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  2. (the Synergic Effect Of Chloroquine And N-acetyl Cysteine In Decreasing Parasitemia And Erythrocyte Malondyaldehyde Level In Balb/c Mice Infected With Plasmodium Berghei)
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