UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 19 / No. 3 / Published : 2001-01

Order : 7, and page :174 - 177

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Original Article :

(lactate dehydrogenase (ldh) during storage)

Author :

  1. Teguh Triyono*1
  2. Umi Solekhah Intansari*2
  3. Caesar Haryo Bimoseno*3
  1. Clinical Pathology Department, Faculty of Medicine, Gadjah Mada University/ Sardjito Hospital Jl. Kesehatan, Sekip Utara Yogyakarta 55281, Indonesia
  2. Clinical Pathology Department, Faculty of Medicine, Gadjah Mada University/ Sardjito Hospital Jl. Kesehatan, Sekip Utara Yogyakarta 55281, Indonesia
  3. Clinical Pathology Department, Faculty of Medicine, Gadjah Mada University/ Sardjito Hospital Jl. Kesehatan, Sekip Utara Yogyakarta 55281, Indonesia

Abstract :

During storage, erythrocytes suffered from biomechanical alterations called the “storage lesion”, which may caused hemolysis. The hemolysis released LDH into the plasma. The LDH that was  released during hemolysis made it an adequate instrument to assess the quality of in vitro blood products. The aims of this study were to analyse the alteration of LDH level at day 1, 3, 7, 14, and 28 in the WB and PRC, to analyse the  correlation between LDH level with  storage duration, and also to analyse  enhancement differences of LDH level between WB and PRC.This research was an observational study with a cross-sectional design. As  the samples there were 11 bags of WB and 10 bags of PRC. Blood products were kept in bloodbank  with the temperature range of 2-6°C. The LDH level was measured with the Beckman Chemistry Analyzer. There were statistically significant alterations of LDH level started from day 7 of storage in both blood products (p<0.05). The significant strong correlation between LDH level with the storage duration were found r=0.772; r=0.835(p<0.05) in WB and PRC respectively. The enhancement differences were found to be higher and significant in the PRC than in the WB started from day 7 of storage (p<0.05). As conclusion, LDH in WB and PRC were signifantly increased during storage, and correlate with storage duration.     Selama penyimpanan, eritrosit akan mengalami perubahan biomekanika yang disebut jejas penyimpanan (storage lesion). Jejas penyimpanan dapat membuat eritrosit mengalami pecah sel darah merah (hemolisis). Hemolisis akan melepaskan LDH ke dalam plasma. Kadar  LDH yang tinggi melepaskan eritrosit dan membuat LDH menjadi alat penilai mutu hasil darah in vitro. Tujuan penelitian ini adalah untuk mengetahui lewat menganalisis peningkatan kadar LDH hasil darah WB dan PRC pada lama penyimpanan satu (1), tiga (3), tujuh (7), 14, dan 28 hari. Di samping itu juga untuk mengetahui lewat menganalisis kenasaban antara kadar LDH dan lama penyimpanan WB dan PRC, serta menganalisis perbedaan peningkatan kadar LDH antara  WB dan PRC. Penelitian ini menggunakan metode observasional dengan desain potong silang. Sampel yang digunakan adalah sebanyak 11 kantong hasil darah WB dan 10 buah dari PRC. Hasil darah disimpan di dalam bank darah yang bersuhu 2-6°C,  dan kemudian sampel diambil setelah tersimpan selama hari ke-1, 3, 7, 14, dan 28. Kadar LDH diukur menggunakan Beckman Chemistry Analyzer dalam satuan IU/L. Mulai hari ke-7 penyimpanan terdapat peningkatan bermakna kadar LDH di kedua hasil darah (p < 0,05). Di samping itu didapatkan kenasaban yang kuat dan bermakna antara kadar LDH dan lama penyimpanan WB dan PRC berturut-turut r=0,772 dan r=0,835 (p<0,05). Peningkatan kadar LDH PRC lebih tinggi dan bermakna dibandingkan dengan kadar LDH WB mulai hari ke-7 penyimpanan (p<0,05). Kadar LDH hasil darah WB dan PRC terdapat peningkatan bermakna setelah penyimpanan selama tujuh (7), 14, dan 28 hari. Kadar LDH didapatkan bernasab kuat dan bermakna dengan lama penyimpanan. Peningkatan kadar LDH PRC lebih tinggi dan bermakna dibandingkan dengan WB selama penyimpanan selama tujuh (7), 14, dan 28 hari.

Keyword :

Storage lesion, hemolysis, LDH,


References :

Campbell-Lee, Sally A, Ness PM,(2007) Specific Blood Components. Blood Banking and Transfusion Medicine Philadelphia : Elsevier

Greer JP, Lee GR, Foerster J, Lukens J, Paraskevas F, and Rodgers GM,(1999) Wintrobe’s Clinical Hematology Philadelphia : Lippincott Williams & Wilkins

Stiene-Martin EA, Lotspeich-Steininger CA, Koepke JA,(1999) Clinical Hematology Philadelphia : Lippincott

Chaudary R, and Katharia R,(2011) Oxidative Injury as Contributory Factor For Red Cells Storage Lesion during Twenty Eight Days of Storage USA : Blood Transfusion

MarjaniA, Moradi A, and Ghourcaie AB,(2007) Alterations in Plasma Lipid Peroxidation and Erythrocyte Superoxide Dismutase and Glutathione Peroxidase Enzyme Activities During Storage of Blood Asian : Asian Journal of Biochemistry





Archive Article

Cover Media Content

Volume : 19 / No. : 3 / Pub. : 2013-01
  1. {(active Caspase-3 In Acute Myeloid Leukaemia (aml) And Acute Lymphoblastic Leukaemia (all)}
  2. (principle Modification Of β-glucan Detection From Candida Albicans In Serum)
  3. Apoptosis Index Between Females And Males In Regular Hemodialysis
  4. [iron Deficiency In Pregnant Women By Haemoglobin Reticulocyte (ret-he)]
  5. (higher Level Of Ctx In Osteoporotic Women Compared To Normal And Osteopenic Women)
  6. (cystatin C, Hba1c And Albumin Creatinine Ratio)
  7. (lactate Dehydrogenase (ldh) During Storage)
  8. (cd4+ T Lymphocyte As A Prognosis Predictor In Sepsis Patients)
  9. (angiotensin Ii On Adipocytes Culture Exposed With High Glucose)
  10. (the Panleucogating Method For Lymphocyte Cd4 Counting In Hiv Patients)
  11. (c3c Serum Complement And Blood T-cd4+ Lymphocyte)
  12. (cell Based Hemostatis – In Vivo)
  13. Neonatal Acute Myeloid Leukaemia

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