UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 12 / No. 2 / Published : 2006-03

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Original Article :

Analysis of acid fast bacilli (afb) findings and concentrated slides in suspected tuberculosis

Author :

  1. Elisabeth Frida*1
  2. S. Ibrahim*2
  3. Hardjoeno*3
  1. Bagian Patologi Klinik Fakultas Kedokteran Universitas Hasanuddin – RS Dr. Wahidin Sudirohusodo Makassar. Alamat kantor: 1. Unit Pelayanan Laboratorium RS Wahidin Sudirohusodo Jl Perintis Kemerdekaan Km 10. Makassar Telp 0411-582678 2. Bagian Patologi Klinik Fakultas Kedokteran Unhas Jl Perintis Kemerdekaan Km 10. Makassar Telp 0411-586010 Alamat Rumah: Jl. Dr. Wahidin Sudirohusodo (Jl. Gassing Dg Tiro) No. 78 B. Sungguminasa Kab. Gowa. Telp. 0411-841199, 081342554711
  2. Bagian Patologi Klinik Fakultas Kedokteran Universitas Hasanuddin – RS Dr. Wahidin Sudirohusodo Makassar. Alamat kantor: 1. Unit Pelayanan Laboratorium RS Wahidin Sudirohusodo Jl Perintis Kemerdekaan Km 10. Makassar Telp 0411-582678 2. Bagian Patologi Klinik Fakultas Kedokteran Unhas Jl Perintis Kemerdekaan Km 10. Makassar Telp 0411-586010 Alamat Rumah: Jl. Dr. Wahidin Sudirohusodo (Jl. Gassing Dg Tiro) No. 78 B. Sungguminasa Kab. Gowa. Telp. 0411-841199, 081342554711
  3. Bagian Patologi Klinik Fakultas Kedokteran Universitas Hasanuddin – RS Dr. Wahidin Sudirohusodo Makassar. Alamat kantor: 1. Unit Pelayanan Laboratorium RS Wahidin Sudirohusodo Jl Perintis Kemerdekaan Km 10. Makassar Telp 0411-582678 2. Bagian Patologi Klinik Fakultas Kedokteran Unhas Jl Perintis Kemerdekaan Km 10. Makassar Telp 0411-586010 Alamat Rumah: Jl. Dr. Wahidin Sudirohusodo (Jl. Gassing Dg Tiro) No. 78 B. Sungguminasa Kab. Gowa. Telp. 0411-841199, 081342554711

Abstract :

Tuberculosis is still an important health problem in Indonesia, being the third place in the world. The diagnosis could be done by direct microscopic method of slides stained with Ziehl Neelsen. The finding of AFB could be improved by decontaminating the specimens to provide a concentrated sample. To analyze the findings of AFB through microscopy and concentrated smear methods, comparing of each to bacteriological culture. Diagnosis was done on 148 patients during May to September 2005, using microscopic method (direct microscopy and concentrated smear) and culture. Analysis of results was done by using SPSS for Window v. 11.5. Of the 148 specimens, 15 (10.1%) and 55 (37.2%) positive AFB were found in direct microscopy and concentrated smears respectively. The sensitivities in direct microscopy and concentrated smears were 22% and 64.8%, while the specificities were 96.8% and 78.7%, positive predictive values (PPV) were 80% and 63.6%, negative predictive values (NPV) 68.4% and 79.5%, accuracy values were 69.5% and 73.6%. The specificity and PPV were higher in direct microscopic method, while positive value, sensitivity, NPV and accuracy value were higher in decontamination, concentrated sample method. Concentrated sample method will increase the accuracy of the finding of Ziehl Neelsenstained AFB so that this method could be assumed better for use.

Keyword :

AFB, direct microscopy, concentrated smear microscopy, tuberculosis,


References :

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Mudihardi E., Kuntaman, Warsito E., Mertaniasih N., Harsono
S., Alimsardjono L., Bagian Mikrobiologi FK Uni



Archive Article

Cover Media Content

Volume : 12 / No. : 2 / Pub. : 2006-03
  1. The Manual And Automatic Tests Results Of Erythrocyte Sedimentation Rate
  2. Drug Resistance Of Mycobacterium Tuberculosis
  3. Analysis Of Acid Fast Bacilli (afb) Findings And Concentrated Slides In Suspected Tuberculosis
  4. The Microorganism Pattern In The Vagina Of Pregnancy Women In Dr. Soetomo Hospital Surabaya
  5. Collection And The Limit Time Of Using Diapers Samples For Urin Related Culture
  6. Laboratoric Diagnosis Of Avian Influenzae (h5n1)
  7. The Habitualis Abortion In Antiphospholipid Syndrome
  8. Evaluation Of Immunochromatography Method For Determination Of Immunoglobulin M And G Anti-dengue In Dengue Pediatric Patients)
  9. Quality Control In Clinical Microbiology
  10. Old People And Diabetes Mellitus
  11. The Analysis Of Serum Osteocalcin Level On Osteopenic And Osteoporotic Subjects

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