UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 15 / No. 3 / Published : 2009-07

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Original Article :

Analysis of the immunochromatography and enzyme-linked immunosorbent assay tests to diagnose helicobacter pylori in dyspepsia

Author :

  1. I Hutagalung*1
  2. Uleng Bahrun*2
  3. Mansyur Arif*3
  4. Rifai Amirudin*4
  5. HAM Akil*5
  1. Departemen Patologi Klinik FK UNHAS - RS Dr. Wahidin Sudirohusodo Makassar Jl. Perintis Kemerdekan Km 10 Talamanrea Makassar
  2. Departemen Patologi Kinik FK - UNHAS - RS. Dr. Wahidin Sudirohusodo Jl. Perintis Kemerdekaan Km 10 Talamarea Makassar
  3. Departemen Patologi Kinik FK - UNHAS - RS. Dr. Wahidin Sudirohusodo Jl. Perintis Kemerdekaan Km 10 Talamarea Makassar
  4. Subdivisi Gastroenterologi, Departemen Penyakit Dalam Fakultas Kedokteran, Universitas Hasanuddin Wahidin Sudirohusodo Jl. Perintis Kemerdekaan Tamalanrea Makassar Telp/Fax +62 411 581226
  5. Subdivisi Gastroenterologi, Departemen Penyakit Dalam Fakultas Kedokteran, Universitas Hasanuddin Wahidin Sudirohusodo Jl. Perintis Kemerdekaan Tamalanrea Makassar Telp/Fax +62 411 581226

Abstract :

Many methods are available to diagnose Helicobacter pylori in patients with dyspepsia, including Enzyme-Linked Immunosorbant Assay (ELISA) and recently used is immnochromatography test. Both of the test are non invasive method, but immunochromatography test can be used in laboratory without semi automatic analyzer. The aim of this study was to compare the immunochromatography and ELISA tests in detecting the possibility of Helicobacter pylori infection. A cross sectional study was done among 49 samples with dyspepsia at Wahidin Sudirohusodo Hospital of Makassar and Prodia Laboratory from March to August 2008. Samples were analyzed with SPSS 14 for Windows Program using Chi Square and Spearman correlation tests. Among 49 samples we found ELISA and immunochromatography tests were positive in 5 samples, ELISA and immunochromatography tests were negative in 33 samples, ELISA tests were borderline and immunochromatography tests were positive in 5 samples, and ELISA tests were borderline and immunochromatography tests were negative in 6 samples. There was a good correlation between ELISA and immunochromatography test with R = 1.000 and p = 0.000. Both results of the immunochromatography and ELISA tests showed high conformity. Both method can be applied to diagnose Helicobacter pylori in patients with dyspepsia. Immunochromatography test can be an alternative method in laboratories who do not apply semi automatic analyzer. The advantages of immunochromatography test can be used for diagnose early infection of Helicobacter pylori.

Keyword :

ELISA, immunochromatography test, Helicobacter pylori, dyspepsia,


References :

Arif M, Amirudin R,(2003) Prevalensi infeksi helicobacter pylori pada populasi normal di Ujung Pandang Makassar : Prodia





Archive Article

Cover Media Content

Volume : 15 / No. : 3 / Pub. : 2009-07
  1. Cd4 Cell Counts With Igm Herpes Simplex-type 2 In Hiv Patients
  2. The Transferrin Saturation Profile Among Anaemic Blood Donors
  3. Iron Deficiency Anemia Of Babies
  4. Patients’ Serum Protein Electrophoresis With Normal Serum Total Protein Level
  5. Inflammatory Marker Hs Crp With Hipertension
  6. Comparison Of Kalium Serum Level With Or Without Insulin Therapy At Type 2 Diabetic Mellitus Patiens
  7. Microalbuminuria Of Type 2 Dm Patients
  8. Analysis Of The Immunochromatography And Enzyme-linked Immunosorbent Assay Tests To Diagnose Helicobacter Pylori In Dyspepsia
  9. Soluble Transferrin Receptor Level In Human Immunodeficiency Virus/acquired Immunodeficiency Syndrome Patients With Anemia
  10. Comparison Of Specthrophotometer Method With Hemocue Method For Haemoglobin Measurement In Leucocytosis Sample
  11. Toll-like Receptor (tlr) And Natural Immunity
  12. A Retinoid Acid Receptor In Acute Promyelocytic Leukemia Auer Rods
  13. Errors During Clinical Laboratoric Procedures

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