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Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 18 / No. 3 / Published : 2012-01

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Original Article :

Antimicrobial susceptibility test of pathogenic aerobic bacteria at the internal medicine ward

Author :

  1. Fedelia Raya*1
  2. Nurhayana Sennang*2
  3. Suci Aprianti*3
  1. Bag PK FK-UNHAS Makassar
  2. Bag PK FK-UNHAS Makassar
  3. Bag PK FK-UNHAS Makassar

Abstract :

Pathogenic bacteria are the major causes of airborne infection at the hospital ward. Nosocomial infection can occur at the opened as well as at the closed room. Nosocomial infection influences the morbidity and mortality in the hospital and need an extra attention, because of the increased number of hospital patients, micro organism mutation and increased of bacteria resistance to antibiotics. The aim of this study was to quantify the number of aerobic bacteria, and to know the pathogenic bacteria identification and its determination on the susceptibility of the antimicrobial problems at the internal medicine ward. This research was carried by a cross sectional study, which performed by collecting air samples in eight internal medicine ward of Dr. Wahidin Sudirohusodo Hospital using Microbiology Air Sampler 100 (MAS 100). The bacterial identification and the antimicrobial susceptibility test (AST) were conducted at the Balai Besar Laboratorium Kesehatan (July to August 2009). In this study were found the numbers of bacteria colonies about 580-6040 CFU/m3. The pathogenic bacteria that identified were Acinetobacter calcoaceticus, Staphylococcus saprohpyticus, Enterobacter hafniae and Stomatococcus mucilaginosus that were sensitive to Amikasin, Gentamicyn, Azitromycin and Norfloxacyn but resistant to Ampicillin.The number of bacterial colonies exceeded the established number standard by Decree of the Indonesian Health Minister. The pathogenic bacteria showed the most sensitive result of AST were Acinetobacter calcoaceticus, Enterobacter hafniae, Stomatococcus mucilaginosus and Staphylococcus saprohpyticus.     Bakteri penyebab penyakit (patogen) merupakan penyebab infeksi terbanyak udara di ruang perawatan rumah sakit. Infeksi  asal rumah sakit (nosokomial) melalui udara ini dapat terjadi dalam keadaan yang terbuka maupun tertutup. Infeksi nosokomial sangat mempengaruhi angka kesakitan dan kematian di rumah sakit dan sangat perlu diperhatikan karena jumlah penderita yang dirawat meningkat, mutasi mikroorganisme demikian pula resistensi bakteri terhadap antibiotik. Tujuan penelitian ini adalah untuk mengetahui jumlah hitungan bakteri aerob, mengenali bakteri penyebab penyakit dan menentukan kepekaan antimikroba khusus di Ruang Perawatan Penyakit Dalam RS Wahidin Sudirohusodo Makassar. Penelitian bersifat potong silang dengan cara mengambil sampel udara di  delapan Ruang Perawatan Penyakit Dalam RS Wahidin Sudirohusodo Makassar menggunakan Microbiology Air Sampler (MAS 100). Perhitungan bakteri aerob, pengenalian bakteri penyebab penyakit dan uji kepekaan dilakukan di Balai Besar Laboratorium Kesehatan (Juli – Agustus 2009). Jumlah koloni bakteri adalah 580 – 6045 CFU/m3. Bakteri penyebab penyakit yang ditemukan adalah Acinetobacter calcoaceticus, Staphylococcus saphropyticus, Enterobacter hafniae dan Stomatococcus mucilaginosus yang semuanya peka terhadap obat Amikasin, Gentamicin, Azitromycin dan Norfloxacin, sedangkan resisten hanya terhadap Ampicilin. Jumlah kelompok bakteri melebihi bakuan yang ditetapkan oleh Peraturan Menteri Kesehatan RI. Urutan bakteri penyebab penyakit yang paling banyak memberikan hasil yang peka terhadap antibiotik keampuhan luas (broadspectrum) yang diuji adalah Acinetobacter calcoaceticus, Enterobacter hafniae, Stomatococcus mucilaginosus dan Staphylococcus saphropiticus.

Keyword :

Aerobic bacteria, pathogen bacteria, antimicrobial susceptibility test, internal medicine ward,


References :

  1. 1. Budiarti LY, Noormuthmainah, Rahmiati, (2007). Jenis bakteri dan jamur kontaminan udara di Ruang Perawatan Penyakit Dalam RSUD Banjar Baru. Jakarta : Jurnal Kedokteran Yarsi
  2. Greenwood D, (2007). Hospital Infection. In Medical Microbiology. Philadelphia : Elsevier Churchill Livingstone
  3. Utama WH, (2009). Infeksi nosokomial. Jakarta : www.klikharry
  4. www.nosormhskt, (2009). Infeksi nosokomial masalah serius bagi pengelolah rumah sakit. Jakarta : www.nosormhskt
  5. 5. Tim Pencegahan dan Pengendalian Infeksi Nosokomial RS Wahidin Sudirohusodo Makassar (PPI-RSWS), (2008). Evaluasi Hasil Surveilans Infeksi Nosokomial. Makassar : 5. Tim Pencegahan dan Pengendalian Infeksi Nosokomial RS Wahidin Sudirohusodo Makassar (PPI-RSWS)


   


Archive Article

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Volume : 18 / No. : 3 / Pub. : 2012-01
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