UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 19 / No. 3 / Published : 2013-01

Order : 3, and page :150 - 155

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Original Article :

Apoptosis index between females and males in regular hemodialysis

Author :

  1. Djoko Santoso*1
  1. Department of Internal Medicine, Airlangga University Faculty of Medicine Dr Soetomo General Hospital, Surabaya, Indonesia

Abstract :

Many reports have documented apoptosis index in hemodialysis patients, but to date, no single study has directly compared the apoptosis index of males to females. Data on mortality rate among hemodialytic patients in the hemodialysis center at the Department of Internal Medicine Dr Soetomo General Hospital, Surabaya, Indonesia show a high number predominated by female patients. Therefore, to answer the question of whether there is a gender difference in apoptosis index, the researcher studied leukocyte responses in male and female hemodialysis patients. The apoptosis index of  the sample was measured by indirect immunoassay method. Cell lyses, followed by immunochemical determination of histone-complexed DNA fragments in a microtiter plate wells. The apoptosis quantization was obtained by determining the amount of colored product spectrophotometrically. One hundred and four non-diabetic subjects who received hemodialysis (HD), and 24 normal controls (NC), were evaluated. The apoptosis index in ESRD patients group and control group showed no significant difference (0.6172 vs 0.4008, p=0.114), neither did it vary in both sexes and age groups. When the sex factor was analyzed (after exclusion from the diabetic ESRD patients), females apoptosis index was significantly higher than that of the males (0.7325 vs 0.55175, p <0.05). In conclusion, apoptosis index in females among non-diabetic patients undergoing hemodialysis is higher than that occur in males and controls.     Banyak laporan telah memuat indeks apoptosis di pasien hemodialisis. Namun sampai saat ini, tidak ada satupun penelitian yang langsung membandingkan indeks apoptosis perempuan terhadap laki-laki. Data angka kematian di antara pasien hemodialisis di pusatnya di Departemen Penyakit Dalam RSU Dr Soetomo, Surabaya, Indonesia cukup tinggi dan lebih banyak pengidap perempuan. Oleh karena itu, untuk menentukan perbedaan terkait jenis kelamin dalam indeks apoptosis, diteliti respons leukosit di pasien hemodialisis laki-laki dan perempuan. Untuk mengetahui indeks apoptosis, sampel diukur dengan metode imunoasai indirek. Lisis sel, diikuti dengan penentuan imunokimawi dari fragmen DNA terhimpun histon dalam piring mikrotiter. Kuantisasi apoptosis dilakukan dengan menentukan jumlah hasilan yang berwarna secara spektrofotometri. Sebanyak 104 subjek non-diabetes yang menjalani hemodialisis (HD) dinilai, dan 24 orang yang sehat (NC) sebagai pembanding. Indeks apoptosis di kelompok pasien ESRD dan kelompok pembanding menunjukkan tidak ada perbedaan yang bermakna (0,6172 vs 0,4008, p = 0.114). Di samping itu juga tidak beragam di kedua jenis kelamin dan kelompok umur. Ketika faktor jenis kelamin dianalisis (setelah tidak disertakan dari pasien ESRD diabetes), indeks apoptosis perempuan lebih tinggi secara bermakna dibandingkan dengan laki-laki (0,7325 vs 0,55175, p <0,05). Berdasarkan telitian ini dapat disimpulkan bahwa di kelompok perempuan non-diabetes yang menjalani hemodialisis, indeks apoptosis lebih tinggi dibandingkan dengan kelompok laki-laki dan pembandingnya.

Keyword :

Apoptosis index, hemodialysis, gender,


References :

Santoso D, Yogiantoro M, Pranawa ,(2007) Mortality risk factos: 3 years mortality after first hospitalization in hemodialysis patients treated in Soetomo Hospital, Surabaya. Indonesia Surabaya : Accessed from: www.jsn.go.jp/AFCKDI2007/abstract/ 5_18.html.

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Archive Article

Cover Media Content

Volume : 19 / No. : 3 / Pub. : 2013-01
  1. {(active Caspase-3 In Acute Myeloid Leukaemia (aml) And Acute Lymphoblastic Leukaemia (all)}
  2. (principle Modification Of β-glucan Detection From Candida Albicans In Serum)
  3. Apoptosis Index Between Females And Males In Regular Hemodialysis
  4. [iron Deficiency In Pregnant Women By Haemoglobin Reticulocyte (ret-he)]
  5. (higher Level Of Ctx In Osteoporotic Women Compared To Normal And Osteopenic Women)
  6. (cystatin C, Hba1c And Albumin Creatinine Ratio)
  7. (lactate Dehydrogenase (ldh) During Storage)
  8. (cd4+ T Lymphocyte As A Prognosis Predictor In Sepsis Patients)
  9. (angiotensin Ii On Adipocytes Culture Exposed With High Glucose)
  10. (the Panleucogating Method For Lymphocyte Cd4 Counting In Hiv Patients)
  11. (c3c Serum Complement And Blood T-cd4+ Lymphocyte)
  12. (cell Based Hemostatis – In Vivo)
  13. Neonatal Acute Myeloid Leukaemia

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