UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 18 / No. 3 / Published : 2012-01

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Original Article :

Association hla-drb1* and hla-dqb1* with serum igm-rf with rheumatoid arthritis

Author :

  1. Joewono Soeroso*1
  2. Ferdinandus Maria Judajana*2
  3. Handono Kalim*3
  1. Divisi Reumatologi, Departemen-SMF Ilmu Penyakit Dalam, Fakultas Kedokteran Universitas Airlangga-RSUD Dr Soetomo, Surabaya
  2. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya
  3. Divisi Reumatologi, Departemen-SMF IlmuPenyakit Dalam, Fakultas Kedokteran UniversitasBrawijaya-RSUD Syaiful Anwar, Malang

Abstract :

Rheumatoid arthritis (RA) is a chronic progressive polygenic autoimmune disease with a high socio-economic burden. Genetic polymorphisms expressed as a shared epitope (SE) at hypervariable regions of HLA-DRB1*01/*04/*10/*14 and linkage disequilibriums of HLA-DRB1*-HLA-DQB1* with some microorganisms, increase the risk of  RA. Shared epitopes are also related with the increase of serum IgM-RF as well. A cross-sectional study on  48 RA patients diagnosed by ACR Criteria 1987 from 6 hospitals in Java and Bali was done to determinethe associations of HLA-DRB1* and HLA-DQB1* alleles with  serum IgM-RF levels. The presence of HLA-DRB1* and HLA-DQB1* alleles were assessed  through  PCR-SSO. Serum IgM-RF levels (IU/mL) were assessed through ELISA. Man-Whitney U tests were used to measure the associations. Value of p<0.05 is considered to be statistically significant. The results showed that mean ranks IgM-RF levels  of subjects who are bearing HLA-DRB1*04 compared to subjects who are not bearing HLA-DRB1*04 were 34.10 IU/ml (n=10) vs 21.97 IU/ml (n=38), p=0.014]. While, the mean ranks of IgM-RF levels  of subjects who are bearing  haplotype combination of HLA-DRB1*04-HLA-DQB1*03 vs subjects who are not bearing haplotype combination of HLA-DRB1*04- HLA-DQB1*03 were 32.89 IU/mL (n=9)] vs 22.56 IU/mL (n=39) (p=0.046)]. It is concluded that there are association of HLA-DRB1*04, and haplotype combination of HLA-DRB1*04-HLA-DQB1*03 with increased levels of IgM-RF among Indonesians with RA.     Artritis reumatoid (RA) merupakan suatu penyakit otoimun kronik progresif dengan dampak sosio-ekonomik yang buruk. Polimorfisme genetik berupa pasangan epitop [shared epitope (SE)] dari alela HLA-DRB1*01/*04*/10*/14 dan gabungan haplotipe HLA-DRB1*-HLA-DQB1*dengan mikroorganisme tertentu meningkatkan kebahayaan timbulnya AR. SE juga terkait dengan peningkatan kadar IgM-RF serum. Sebuah telitian dengan desain potong lintang untuk menentukan pertalian (asosiasi) alela HLA-DRB1* dan HLA-DQB1* dengan kadar IgM-RF serum telah dilaksanakan pada 48 pasien dengan AR yang didiagnosis melalui patokan ACR  1987. Pasien tersebut berasal dari 6 RS di Jawa dan Bali. Alela HLA-DRB1* dan HLA-DQB1* ditentukan melalui PCR-SSO. Kadar IgM-RF (IU/mL) diukur dengan metode ELISA. Uji Man-Whitney U digunakan untuk menganalisis pertalian kadar IgM-RF pada subjek yang membawa SE dibanding yang tidak membawa SE. Nilai p<0.05 dianggap bermakna secara statistik. Hasil analisis menunjukkan rerata peringkat kadar IgM-RF subjek pembawa HLA-DRB1*04 dibanding subjek bukan pembawa HLA-DRB1*04 adalah 34,10 IU/mL (n=10) dibanding 21,97 IU/mL (n=38), p=0,014]. Rerata peringkat kadar IgM-RF pada subjek yang membawa gabungan haplotipe HLA-DRB1*04-HLA-DQB1*03 dibanding subjek yang membawa gabungan haplotipe HLA-DRB1*04-HLA-DQB1*03 adalah 32,89 IU/mL (n=9)] dibanding 22,56 IU/mL (n=39) (p=0,046)]. Kesimpulan dari penelitian ini, terdapat pertalian HLA-DRB1*04 dan gabungan haplotipe HLA-DRB1*04-HLA-DQB1*03 dengan peningkatan kadar IgM-RF pada pasien RA di Indonesia.

Keyword :

Association, HLA-DRB1* , HLA-DQB1* , IgM-RF rheumatoid arthritis,


References :

Gabriel SE, Michaud K,(2009) Epidemiological studies in incidence, prevalence, mortality, and comorbidity of the rheumatic diseases USA : Arthritis Res & Ther

Silman AJ, Pearson JE,(2002) Epidemiology and genetics of rheumatoid arthritis USA : Arthritis Res Ther

Kalim H,(2000) Masalah penyakit reumatik di Indonesia serta upaya penanggulangannya Jakarta : Naskah Lengkap Temu Ilmiah Reumatologi

4. Milicic A, Lee D, Brown MA, Darke C, Wordsworth P,(2002) HLA-DR/DQ Haplotype in rheumatoid arthritis: novel allelic associations in UK Caucasians UK : J Rheumatol

Zanelli E, Breedveld FC, de Vries RR,(2000) HLA class II association with rheumatoid arthritis: facts and interpretations USA : Hum Immunol





Archive Article

Cover Media Content

Volume : 18 / No. : 3 / Pub. : 2012-01
  1. Prothrombin Time And Activated Partial Thromboplastin Time Test’s Result Using Humaclot Va And Sysmex Ca 500
  2. Association Hla-drb1* And Hla-dqb1* With Serum Igm-rf With Rheumatoid Arthritis
  3. Platelets Of Dengue Hemorrhagic Fever
  4. The Diagnostic Value Of Tb Antigen Using Rapid Test Device (tb Ag) For Pulmonary Tuberculosis
  5. Antimicrobial Susceptibility Test Of Pathogenic Aerobic Bacteria At The Internal Medicine Ward
  6. Correlation Of Liver Functions Test, And The Grade Of Dengue Hemorrhagic Fever In Children
  7. Pulmonary Cryptosporidiosis In Tbc Patients
  8. Mycobacterium Tuberculosis And Pcr
  9. Oral Immunization With 38-kda Adhesin Protein Of Mycobacterium Tuberculosis On Cd8+ T Cells In Lung
  10. Thrombocytes Count In Acute Coronary Syndrome Related To Low Molecular Weight Heparin (lmwh)}
  11. Acute Fatty Liver Of Pregnancy
  12. Cold Agglutinins In A Community Acquired Pneumonia Patient

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