UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 19 / No. 2 / Published : 2013-01

Related with : Scholar   Yahoo!   Bing

Original Article :

Clinical manifestion sepsis and nitric oxide level on mice induced by lipopolysaccharide

Author :

  1. Sotianingsih*1
  2. Soeharyo*2
  3. Lisyani S*3
  4. Guntur H*4
  1. RSUD Raden Mattaher Jambi
  2. Program Pascasarjana Undip, Semarang
  3. Bagian Patologi Klinik Undip, Semarang
  4. Bagian Penyakit Dalam UNS,Surakarta

Abstract :

Sepsis remains a health problem because of the high related mortality and morbidity. One of the efforts is the improvement of knowledge about manifestation and pathophysiology of sepsis at the level of molecular biology. Nitric oxide (NO) levels increased in sepsis and this is related to the occurrence of cell apoptosis. The purpose of this study was to know and to see the clinical picture of sepsis and elevated levels of NO in mice Balb / C which were injected with lipopolysaccharide (LPS). In this research, the mice were divided into three (3) groups, group A were injected with saline, group B were injected with LPS 0.1 mg per mouse, while group C was injected with 0.2 mg per mouse. The mice were then measured by clinical parameters from day 0 and day 1, and 3,5 to 7. The levels of NO were assessed by blood sampling as much as 1 cc from the peri orbital plexus and examined by Griess method on days 1, 3, 5, 7. The results of the study showed that from the weight parameter there was a decrease in body weight on day 1 in group B and C and the weight continued to decline until day 3. From the temperature parameter it was shown that the temperature decreased both in group B and C on day 1 but on day 3 and so on varies in both groups. From the piloerection parameters from day 1 to day 7 showed that there was piloerection in both groups. Related to the discharge by peri orbital parameters and diarrhoea many variations, occurred on day 1 peri orbital discharge and diarrhoea was found, but not uniformly in all the mice in each group. On day 5 and 7 the peri orbital discharge and diarrhoea were not visible. NO levels increased mainly on day 3 and the percentage was higher in group C and on day 5 and day 7 the levels were negative. In conclusion in this study it was shown, that the clinical picture is striking, weight loss and increased levels of NO occurs on day 3 of the observation.     Sepsis masih menjadi masalah kesehatan karena tingginya angka kematian dan angka kesakitan. Salah satu upaya untuk pencegahannya adalah menambah pengetahuan sepsis klinis dan patofisiologis di tingkat Biologi Molekul. Kadar NO meningkat saat sepsis dan peningkatannya yang berkaitan dengan apoptosis sel. Tujuan penelitian ini adalah untuk mengetahui dan melihat gambaran sepsis klinis dan peningkatan kadar NO di mencit Balb/C yang disuntik lipopolysaccharide (LPS). Dalam metode penelitian ini mencit dibagi dalam tiga (3) kelompok. Kelompok A adalah kelompok pembanding, kelompok B disuntik LPS 0,1 mg per mencit sedangkan kelompok C 0,2 mg per mencit. Kemudian diukur dengan tolok ukur klinis mulai pada hari ke 0 kemudian hari ke 1, 3,5 dan ke 7, sedangkan kadar NO dinilai pada hari 1, 3, 5, dan 7. Hasil telitian, didasari pengamatan tolok ukur berat badan mencit terjadi penurunan pada hari ke 1. Demikian pula di kelompok B dan C berat badannya terus menurun dan mencapai puncaknya pada hari ke 3. Untuk tolok ukur suhu terjadi penurunan suhu pada kelompok B dan C pada hari ke 1 akan tetapi pada hari ke 3 dan seterusnya terjadi keragaman di kedua kelompok tersebut. Untuk tolok ukur piloerection mulai pada hari ke 1 sampai hari ke 7 terjadi piloerection di kedua kelompok tersebut. sedangkan untuk tolok ukur discharge peri orbital dan diare terdapat banyak keragaman. Kadar NO meningkat terutama pada hari ke 3 dan secara persentase di kelompok C lebih tinggi, tetapi mulai pada hari ke 7 kadar kembali turun. Didasari telitian ini dapat disimpulkan, bahwa tolok ukur yang dapat dipakai adalah penurunan berat badan dan peningkatan kadar NO yang puncaknya terjadi pada hari ke 3.

Keyword :

Mice, sepsis, lipopolysaccharide, clinical, nitric oxide,


References :

Ayala A, Wesche-Soldato DE, Perl M, Lomas-Neira JL, Swan R, Chung C,(2007) Blockade of apoptosis as a rational therapeutic strategy for the treatment of sepsis USA : Novartis Found Symp

Vincent J, Zhang H, Szabo C, Preiser J,(2000) Effect of nitric oxide in septic shock (Clinical commentary) USA : Am J Respir Crit Care Med

Chung HT, Pae H, Choi B, Billiar TR, Kim Y,(2001) Nitric oxide as a bioregulator of apoptosis (breakthoughs and views) USA : Biochem Biophys Res Commun

Wesche-Soldato CE, Lomas-Neira JL, Perl M, Jones L, Chung C, Ayala A,(2005) The role and regulation of apoptosis in sepsis USA : J of Endotoxin Res

Hotchiss RS, Tinsley KW, Karl IE,(2003) Role of apoptotic cell death in sepsis USA : Scan J Dis





Archive Article

Cover Media Content

Volume : 19 / No. : 2 / Pub. : 2013-01
  1. Clinical Manifestion Sepsis And Nitric Oxide Level On Mice Induced By Lipopolysaccharide
  2. Gandarusa (justicia Gendarussa Burm.f.) Water And Expression Of Hyaluronidase Gene By Pcr Analysis
  3. Proteinuria In Stroke With And Without Diabetic
  4. The Stewart’s Approach In Blood Ph Underlying Metabolic Acidosis
  5. Microbes And Antimicrobial Sensitivity In Open Fracture
  6. Katekin From Green Tea Leaves (camellia Sinensis) To Malondialdehyde (mda) And Super Oxide Dismutase (sod)
  7. Procalcitonin And Interleukin-6 In Sepsis Systemic Inflammatory Response Syndrome (sirs)
  8. Gram Negative And Gram Positive Aerobic Bacteria Identification Using Conventional And Automatic Method
  9. Immature Platelet Fraction (ipf) And Thrombopoietin In Liver Cirrhosis
  10. Eosinopenia And Procalcitonin In Sepsis
  11. C-x-c Receptor 4 (cxcr4) In Metastasis Of Breast Cancer
  12. Hairy Cell Leukaemia

Information CPML