UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 19 / No. 1 / Published : 2012-01

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Original Article :

Comparation measurement triglycerides, glycerol blanking and non glycerol blanking in liver cirrhosis

Author :

  1. Sri Widyaningsih*1
  2. Leonita Anniwati*2
  3. Juli Soemarsono*3
  1. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya
  2. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya
  3. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya

Abstract :

Liver cirrhosis is a chronic liver disease with fibrosis and impairment of function. In liver disease increasing endogenous free glycerol has been found which constitutes a source of interference in the measurement of (TG). The aim of this study is to compare the glycerol blanking (TG-GB) which does not interfere with endogenous free glycerol to non glycerol blanking (TG-NGB) method in measuring TG in liver cirrhosis patients. In this study, sera were obtained from 22 liver cirrhosis and 30 healthy persons. All sera were measured for TG using TMS 1024i (Tokyo Boeki Medical System) with glycerol blanking (TG-GB) to non glycerol blanking (TG-NGB) method. In addition to this study, measurement of free glycerol was also compared to difference calculated TG-NGB and TG-GB in liver cirrhosis patients. The mean of TG-GB and TG-NGB measurements from healthy persons was 115.30±70.35 mg/dL and 121.27±67.14 mg/dL (p= 0.108), respectively. The mean of TG-GB and TG-NGB measurements from liver cirrhosis patients was 101.09± 78,55  mg/dL and 120.91±79,44 mg/dL (p<0.0001). There was a significant difference between TG-GB and TG-NGB, and there was no significant difference between measurement of free glycerol to difference calculated TG-NGB and TG-GB in liver cirrhosis (p= 0.784). However, there was a significant correlation between TG-GB and TG-NGB in healthy persons and liver cirrhosis patients. (p<0.0001). The measurement of TG-GB is more accurate than TG-NGB method in liver cirrhosis patients.     Pengerasan hati/sirosis hepatis (SH) merupakan penyakit hati kronik yang ditandai dengan pembentukan jaringan ikat (fibrosis) dan gangguan fungsi hati. Peningkatan kadar gliserol bebas endogen di penyakit hati dapat mempengaruhi pemeriksaan trigliserida (TG). Penelitian ini bertujuan membandingkan pemeriksaan TG metode mengosongkan gliserol /glycerol blanking (TG-GB) dan mengosongkan non gliserol/non glycerol blanking (TG-NGB)  di penderita SH. Sampel penelitian adalah serum 22 penderita SH dan 30 orang sehat sebagai pembanding. Setiap sampel diperiksa kadar TG menggunakan alat TMS 1024i (Tokyo Boeki Medical System) dengan metode glycerol blanking (TG-GB) dibandingkan metode non glycerol blanking (TG-NGB). Penelitian ini juga membandingkan kadar gliserol bebas dengan pemeriksaan langsung dibandingkan dengan kadar gliserol bebas dari selisih antara hasil TG-NGB dan TG-GB di penderita SH. Rerata TG-GB dan TG-NGB di orang sehat masing-masing 115,30±70,35 mg/dL dan 121,27±67,14 mg/dL (p= 0,108). Rerata TG-GB dan TG-NGB di SH masing-masing 101,09±78,55 mg/dL dan 120,91±79,44 mg/dL. Didapatkan ada perbedaan bermakna hasil TG metode TG-GB dan TG-NGB pada SH (p<0,0001). Kadar gliserol bebas dari hasil pemeriksaan langsung dengan selisih antara TG-NGB dan TG-GB di SH tidak ada perbedaan bermakna (p= 0,784). Berdasarkan analisis regresi linier didapatkan kenasaban yang baik antara TG-GB dan TG-NGB (p<0,0001) baik di orang sehat maupun SH. Pemeriksaan TG dengan metode TG-GB lebih teliti dibandingkan dengan metode TG-NGB di penderita SH.

Keyword :

Triglyceride, glycerol, liver cirrhosis, free glycerol, glycerol blanking,


References :

Siti Nurdjanah,(2006) Buku Ajar Ilmu Penyakit Dalam Jakarta : FKUI Press

Remaley, et al,(2005) Lipids and Lipoproteins. Clinical Chemistry principles, procedures, correlations USA : Lippincott Williams and Wilkins

Stein EA, et al,(1995) NCEP Recommendation for Triglyceride Measurement: Executive Summary USA : Clinical Chemistry

Hilbert T & Lifshitz MS,(2006) Lipids and Dyslipoproteinemia. Henry’s Clinical diagnosis and management by laboratory methods Philadelphia : Saunders Elsivier

Robert et al,(2000) Lipolysis and lipid oxidation in cirrhosis and after liver transplantation USA : Am J Physiol Gastrointest Liver Physiol





Archive Article

Cover Media Content

Volume : 19 / No. : 1 / Pub. : 2012-01
  1. Pulmonary Cryptosporidiosis In Hiv And Aids
  2. Aerob Bacteria And Antimicrobial Susceptibility
  3. Plasma Volume And Factor Viii In Cryoprecipitated
  4. Comparation Measurement Triglycerides, Glycerol Blanking And Non Glycerol Blanking In Liver Cirrhosis
  5. The Residue Of Leukocyte In Thrombocyte Concentrate
  6. Antimicrobial Sensitivity Of Blood Culture In Neonatal Sepsis
  7. Neutrophils Lymphocyte Ratio In Acute Appendicitis
  8. Curcuma Zedoaria And Morinda Citrifolia As Hepatoprotector Against Carbontetrachloride
  9. Mean Platelet Volume In Stroke
  10. Dengue Serotypes Distribution In Surabaya In The Year 2012
  11. M.tuberculosis In Innate Immunity Associated With The Receptors
  12. Ovarian Dysgerminomas Cancer
  13. Information System In Laboratory Services

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