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Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 15 / No. 3 / Published : 2009-07

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Original Article :

Comparison of specthrophotometer method with hemocue method for haemoglobin measurement in leucocytosis sample

Author :

  1. Basti Andriyoko*1
  2. Leni Lismayanti*2
  3. Delita Prihatni*3
  1. Subbagian Hematologi Patologi Klinik Fakultas Kedokteran Universitas Padjadjaran Rumah Sakit Dr. Hasan Sadikin Bandung
  2. Subbagian Hematologi Patologi Klinik Fakultas Kedokteran Universitas Padjadjaran Rumah Sakit Dr. Hasan Sadikin Bandung
  3. Subbagian Hematologi Patologi Klinik Fakultas Kedokteran Universitas Padjadjaran Rumah Sakit Dr. Hasan Sadikin Bandung

Abstract :

Background and objective: The measurement of haemoglobin was carried out by using hematology analyzer with specthrophotometer method which measured light absorbance at 540 nm. However, this measurement affected by increase turbidity as a result of leucocytosis. The turbidity can be eliminated by using HemoCue method that detect absorbance at 570 nm and 880 nm. The aim of this study was to compare the measurement of haemoglobin obtained between specthrophotometer method and HemoCue method. Materials and method: Blood EDTA sample that have been measured with MEK-6318K Nihon Kohden hematology analyzer for haemoglobin levels with spectrophotometer methods with leucocyte > 100.000/mm3 were included in this study. Blood sample are measured again for the haemoglobin level with HemoCue B-Haemoglobin Analyzer. This study was conducted at Clinical Pathology Laboratory, Dr. Hasan Sadikin Hospital Bandung from August–October 2008. Result: Seventeen leucocytosis sample were enroled in this study. The mean haemoglobin level from specthrophotometer method is higher than HemoCue method, however there was no statistically significant difference between haemoglobin result from specthrophotometer method and HemoCue method (p = 0.742 > a = 0.05). Conclusion: There was no significant difference beetween specthrophotometer method and HemoCue method for haemoglobin measurement of leucocytosis sample.

Keyword :

Haemoglobin, specthrophotometer, HemoCue method, leucocytosis,


References :

  1. Weddimg ME, Toenjes SA, (1998). Medical Laboratoy Procedures. Philadhelpia : FA. Davis Company


   


Archive Article

Cover Media Content

Volume : 15 / No. : 3 / Pub. : 2009-07
  1. Cd4 cell counts with igm herpes simplex-type 2 in hiv patients
  2. The transferrin saturation profile among anaemic blood donors
  3. Iron deficiency anemia of babies
  4. Patients’ serum protein electrophoresis with normal serum total protein level
  5. Inflammatory marker hs crp with hipertension
  6. Comparison of kalium serum level with or without insulin therapy at type 2 diabetic mellitus patiens
  7. Microalbuminuria of type 2 dm patients
  8. Analysis of the immunochromatography and enzyme-linked immunosorbent assay tests to diagnose helicobacter pylori in dyspepsia
  9. Soluble transferrin receptor level in human immunodeficiency virus/acquired immunodeficiency syndrome patients with anemia
  10. Comparison of specthrophotometer method with hemocue method for haemoglobin measurement in leucocytosis sample
  11. Toll-like receptor (tlr) and natural immunity
  12. A retinoid acid receptor in acute promyelocytic leukemia auer rods
  13. Errors during clinical laboratoric procedures