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Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 21 / No. 1 / Published : 2014-01

TOC : 10, and page :45 - 49

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Original Article :

Cryptococcal antigen of acquired immune deficiency syndrome with lateral flow assay and cryptococcus antigen latex agglutination system

Author :

  1. artiti*1
  2. Indrati AR*2
  3. Ganiem AR*3
  1. PPDS Patologi Klinik, Fakultas Kedokteran, Universitas Padjajaran, Bandung.
  2. Departemen/UPF Patologi Klinik Fakultas Kedokteran Universitas Padjajaran
  3. Departemen/UPF Ilmu Penyakit Syaraf Fakultas Kedokteran Universitas Padjajaran RSUP Dr Hasan Sadikin, Bandung

Abstract :

Cryptococcosis is the fourth most common opportunistic infection among Acquired Immune Deficiency Syndrome (AIDS) patients. About 100% mortality has been reported within two weeks in patient with cryptococcal meningitis but without specific treatment. The aim of the study was to compare Cryptococcal antigen (CrAg) detection between Cryptococcus Antigen Latex Agglutination System (CALAS) and Lateral Flow Assay (LFA) among AIDS patients. This research was designed as comparative analytic with cross sectional study on 56 serum derived from AIDS patient who visited Teratai Clinic before ARV therapy initiation and who had never been diagnosed as Cryptococcal meningitis. Each sample was tested for CrAg with CALAS and LFA according to the manufacturer instructions. This study was conducted in the Clinical Pathology Laboratory at Dr. Hasan Sadikin Hospital/Centre Research Unit (CRU) Medical Faculty, Padjajaran University between December 2012March 2013. The statistical analysis was done using Chi square test. The result showed that there was no significant difference between CALAS and LFA method with p=0.596 (p>0.05), the positive probability of CrAg detection using LFA was 0.75 times compared to the CALAS method. For the CrAg detection in the AIDS patients there was no significant difference between CALAS and LFA and the positive probability of CrAg for LFA was detected about 0.75 times compared to the CALAS method. Kriptokokosis merupakan infeksi oportunistik terbanyak keempat di pasien Acquired Immune Deficiency Syndrome (AIDS). Kematian pasien meningitis Kriptokokus dilaporkan 100% terjadi dalam dua minggu tanpa pengobatan. Tujuan kajian ini adalah untuk mengetahui bandingan periksaan Cryptococcal antigen (CrAg) antara Cryptococcus Antigen Latex Agglutination System (CALAS) dan Lateral Flow Assay (LFA) di pasien AIDS. Penelitian ini merupakan analisis perbandingan dengan rancangan potong silang terhadap 56 serum pasien AIDS yang berobat di klinik Teratai RSUP Dr. Hasan Sadikin yang belum mendapat pengobatan anti retroviral (ARV) dan belum didiagnosis meningitis Kriptokokus. Pemeriksaan untuk mendeteksi CrAg dilakukan dengan aglutinasi CALAS (Meredien, EIA) dan LFA (IMMY,USA) di setiap sampel. Penelitian ini dilakukan di Laboratorium Patologi Klinik Dr. Hasan Sadikin/Laboratorium Penelitian Pengabdian Masyarakat Fakultas Kedokteran Universitas Padjajaran (LPPM FK UNPAD) pada masa waktu antara bulan Desember 2012Maret 2013. Analisis statistik menggunakan uji X2 (chi –square). Pada pemeriksaan antara metode CALAS dan LFA tidak terdapat perbedaan bermakna [p=0,596 (p>0,05)] dan peluang mendapat hasil positif untuk mendeteksi CrAg dengan yang metode LFA 0,75 kali dibandingkan dengan cara yang pertama. Dalam kajian pemeriksaan CrAg antara menggunakan metode CALAS dan LFA tidak terdapat perbedaan yang bermakna. Di samping itu pemeriksaan LFA memiliki peluang untuk mendapatkan hasil positif dalam mendeteksi CrAg 0,75 kali dibandingkan dengan metode CALAS untuk serum pasien AIDS.

Keyword :


References :

  1. Schop J., (2007). Protective immunitiy Against Cryptococcus Neoformans infection.. Vol.10 No.1 Hal: 35-43. : McGill J of Med
  2. Jarvis JN, (2007). Harrison TS. HIV-associated cryptococcal meningitis. Vol. 21 No.1 Hal: 2119-29 : AIDS Journal.


Archive Article

Cover Media Content

Volume : 21 / No. : 1 / Pub. : 2014-01
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  2. Correlation plasma fibrinogen with intima-media thickness of carotid artery in diabetes mellitus
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  4. Potassium in multidrug resistance tuberculosis with kanamycin
  5. Safe blood and voluntary non-remunerated blood donors
  6. Mean platelet volume in diabetes mellitus
  7. Ratio of urinary uric acid levels and serum uric acid in type 2 diabetes mellitus
  8. Reticulocyte hemoglobin level of absolute iron deficiency anemia and nonabsolute iron deficiency anemia in end state renal disease undergoing maintenance hemodialysis
  9. Immature platelet fraction in dengue fever and dengue hemorrhagic fever
  10. Cryptococcal antigen of acquired immune deficiency syndrome with lateral flow assay and cryptococcus antigen latex agglutination system
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