UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 19 / No. 1 / Published : 2012-01

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Original Article :

Dengue serotypes distribution in surabaya in the year 2012

Author :

  1. Aryati*1
  2. Puspa Wardhani*2
  3. Benediktus Yohan*3
  4. Eduardus Bimo Aksono H*4
  5. R. Tedjo Sasmono*5
  1. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya, 2Institute of Tropical Disease, Airlangga University, Surabaya
  2. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya, 2Institute of Tropical Disease, Airlangga University, Surabaya
  3. Lembaga Biologi Molekuler Eijkman
  4. 2Institute of Tropical Disease, Airlangga University, Surabaya
  5. Lembaga Biologi Molekuler Eijkman

Abstract :

The characteristics of epidemic dengue often presented as periods of hyperendemicity or as the co-circulation of multiple dengue serotypes. Surabaya is an endemic city for Dengue virus (DENV) transmission. Previous study of DENV distribution in 2008-2009 revealed the predominance of DENV-2. DENV serotypes distribution is known to be dynamic and serotype predominance may change through time. This study aims to determine and follow the circulation of DENV serotype in Surabaya in 2012. We recruited 154 dengue-suspected patients attending Dr. Soetomo Hospital during February until August 2012. Dengue cases were confirmed by IgG and IgM serology tests and NS1 antigen detection. Serologically-positive samples were further analyzed using two-steps reverse transcriptase-polymerase chain reaction (RT-PCR) and viruses were isolated by propagation in C6/36 mosquito cell line. Seventy one cases (46.1%) were detected as DENV positive infection. Serotyping revealed that 61 samples have monotypic infection with one of all four of DENV serotypes and 10 samples have mix-infections. Overall serotyping result observed the predominance of DENV-1 (60.56%). Our result revealed the circulation of all four serotypes of DENV and the presence of serotype exchange in Surabaya in 2012. Annual change of predominant serotype and the presence of multiple infections may play an important role in the transmission of dengue infection. This information is valuable to dengue surveillance in the region. Therefore, the laboratory diagnosis of DENV serotype should be routinely performed to follow the dynamic of dengue disease.      Ciri epidemiologis penyebaran penyakit dengue sering ditandai dengan terjadinya hiperendemisitas atau kemunculan beragam serotipe dengue secara bersamaan. Surabaya merupakan kota endemis untuk infeksi virus dengue. Penelitian terdahulu pada tahun 2005-2009 menunjukan dominasi virus dengue DEN-2. Dominasi serotipe virus dengue di suatu tempat adalah dinamis dan pergantian dominasi terjadi sepanjang waktu. Penelitian ini bertujuan untuk mengetahui dan menentukan peredaran serotipe dengue di Surabaya pada tahun 2012. Sejumlah 154 sampel demam dengue (DD) dan demam berdarah dengue (DBD) dikumpulkan mulai bulan Februari sampai Agustus 2012 di RSUD Dr Soetomo Surabaya. Kasus-kasus dengue dideteksi secara serologi dengan pemeriksaan antibodi IgG dan IgM antidengue dan antigen NS1 ELISA dari Panbio. Sampel-sampel hasil positif dilanjutkan dianalisa dengan reverse-trancriptase polymerase chain reaction (RT-PCR) untuk menentukan serotipe dengue. Sampel tersebut juga dikultur untuk mengisolasi virus di sel nyamuk C6/36. Dari sampel yang diperiksa, didapatkan sebanyak 71 sampel (46,10%) positif. Dari 71 sampel, 61 di antaranya terinfeksi serotipe tunggal dengan berbagai serotipe yang berbeda namun didominasi oleh DEN-1. Sedangkan 10 sampel terdeteksi dengan infeksi ganda. Hasil serotyping menunjukkan terjadinya pergeseran serotipe virus dengue. Keseluruhan hasil di atas menunjukkan bahwa keempat serotipe virus dengue dijumpai di Surabaya. Perubahan dominasi serotipe serta keberadaan infeksi ganda setiap tahun memegang peran penting dari penyebaran infeksi. Keterangan ini sangat bermanfaat untuk tatanan dan diagnosis laboratorik serotipe dengue sehingga distribusi serotipe tersebut sebaiknya diteliti secara berkala.

Keyword :

Dengue, serotypes, Surabaya, 2012,


References :

WHO,(1997) Dengue haemorrhagic fever: diagnosis, treatment, prevention and control Geneva : WHO

Dinas Kesehatan Propinsi Jawa Timur,(2008) Situasi penyakit DBD Propinsi Jatim dan kebijakan program P2 DBD Surabaya : Dinkes Prop Jatim

3. Anoop M, Issac A, Mathew T, Philip S, Kareem NA, Unnikrishnan R, Sreekumar E,(2010) Genetic characterization of dengue virus serotypes causing concurrent infection in an outbreak in Ernakulam, Kerala, South India India : South India. Indian J Exp Biol

4. Salda LTD, Parquet MDC, Matias RR, Natividad FF, Kobayashi N, Morita K,(2005) Molecular epidemiology of dengue 2 viruses in the Philippines: genotype shift and local evolution USA : Am J Trop Med Hyg

5. Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV,(1992) Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction USA : J Clin Microbiol





Archive Article

Cover Media Content

Volume : 19 / No. : 1 / Pub. : 2012-01
  1. Pulmonary Cryptosporidiosis In Hiv And Aids
  2. Aerob Bacteria And Antimicrobial Susceptibility
  3. Plasma Volume And Factor Viii In Cryoprecipitated
  4. Comparation Measurement Triglycerides, Glycerol Blanking And Non Glycerol Blanking In Liver Cirrhosis
  5. The Residue Of Leukocyte In Thrombocyte Concentrate
  6. Antimicrobial Sensitivity Of Blood Culture In Neonatal Sepsis
  7. Neutrophils Lymphocyte Ratio In Acute Appendicitis
  8. Curcuma Zedoaria And Morinda Citrifolia As Hepatoprotector Against Carbontetrachloride
  9. Mean Platelet Volume In Stroke
  10. Dengue Serotypes Distribution In Surabaya In The Year 2012
  11. M.tuberculosis In Innate Immunity Associated With The Receptors
  12. Ovarian Dysgerminomas Cancer
  13. Information System In Laboratory Services

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