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Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 21 / No. 2 / Published : 2015-03

TOC : 2, and page :115 - 119

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Original Article :

Detection in tetrazolium microplate assay culture methods from pulmonary tuberculosis suspected sputum

Author :

  1. Sylvia Rachmayati*1
  2. Tiene Rostini*2
  3. Ida Parwati*3
  4. Rita Rachmayanti*4
  1. Departemen Patologi Klinik Fakultas Kedokteran Universitas Padjadjaran/Rumah Sakit Dr. Hasan Sadikin Bandung
  2. Departemen Patologi Klinik Fakultas Kedokteran Universitas Padjadjaran/Rumah Sakit Dr. Hasan Sadikin Bandung
  3. Departemen Patologi Klinik Fakultas Kedokteran Universitas Padjadjaran/Rumah Sakit Dr. Hasan Sadikin Bandung
  4. Departemen Patologi Klinik Fakultas Kedokteran Universitas Padjadjaran/Rumah Sakit Dr. Hasan Sadikin Bandung

Abstract :

The definitive diagnosis of pulmonary tuberculosis is the discovery of Mycobacterium tuberculosis from sputum culture, but the conventional culture methods using Ogawa media require between 3−10 weeks detection time. Therefore it is needed a prompt diagnostic tools to shorten the detection time. Tetrazolium microplate assay (TEMA) that used tetrazolium bromide as a growth indicator also use mitochondrial dehydrogenate enzymes in the mitochondria of living M. tuberculosis may reduce yellow tetrazolium bromide into purple formazan crystals. The aim of this study was to know the validity and speed of time detection of M. tuberculosis growth by analyzing it. This study was carried out from November 2012 up to February 2013, which obtained 105 subjects conducted in the Department of Clinical Pathology at Dr. Hasan Sadikin Hospital with a cross sectional study design. The subjects consisting of sputum sample from patients who suspected pulmonary TB which is examined for culture of M. tuberculosis with TEMA method using Ogawa media. Statistical analysis was used a 2×2 table to test the validity and Mann Whitney test for the differences in growth detection time. The validity test of TEMA method got the sensitivity of 90.4% and specificity of 96.2%. The detection time of M. tuberculosis growth in TEMA methods was found fastest in the third day while from the Ogawa media cultur was found on the 13th day with the M. tuberculosis growth media using TEMA methods detected in 12 days. While for those cultured on Ogawa’s media the mean duration is 22 days (p<0.001). Based on this study, can be concluded the examination of M. tuberculosis culture from sputum patient suffer of pulmonary TB with with TEMA method has given high validity and faster in the time detection for the diagnosis of pulmonary TB. Diagnosis pasti tuberkulosis (TB) paru adalah ditemukannya Mycobacterium tuberculosis di biakan dahak, tetapi metode biakan konvensional media Ogawa memerlukan waktu antara 3−10 minggu, sehingga diperlukan alat diagnostik yang lebih cepat. Tetrazolium Microplate Assay (TEMA) menggunakan tetrazolium bromida sebagai petunjuk pertumbuhan dengan memanfaatkan enzim mitochondrial dehydrogenase di mitokondria M. tuberculosis hidup yang dapat mengubah tetrazolium bromida berwarna kuning menjadi formazan kristal ungu. Tujuan penelitian untuk mengetahui kesahihan dan kecepatan waktu deteksi pertumbuhan M. tuberculosis dengan metode biakan TEMA. Penelitian dilakukan antara bulan November 2012−Februari 2013 di 105 subjek penelitian di Departemen Patologi Klinik Rumah Sakit Dr. Hasan Sadikin dengan rancangan penelitian potong lintang. Subjek penelitian adalah pasien terduga TB paru dengan sampel penelitian dahak yang dibiakkan dengan metode TEMA menggunakan media Ogawa. Analisis statistik menggunakan tabel 2×2 untuk uji kesahihan dan Mann Whitney untuk perbedaan waktu deteksi pertumbuhan. Uji kesahihan pemeriksaan biakan M. tuberculosis metode TEMA mendapatkan nilai kepekaan 90,4% dan kekhasan 96,2%. Deteksi pertumbuhan M. tuberculosis di biakan metode TEMA paling cepat pada hari ke-3, sedangkan di media Ogawa hari ke-13. Lama pertumbuhan M. tuberculosis di biakan metode yang sama 12 hari, sedangkan di media Ogawa 22 hari (p<0,001). Berdasarkan pemeriksaan biakan M. tuberculosis dahak pasien terduga TB paru dengan metode TEMA memiliki kesahihan yang tinggi dan waktu deteksi lebih cepat untuk kepastian diagnosis TB paru.

Keyword :

Cultures TEMA method, faster detection, sputum, suspect pulmonary tuberculosis,


References :

  1. WHO, (2012). Global Tuberculosis Report 2012. Ed. ke-17,. 1–14. : WHO Press
  2. Raviglione MC, O’Brien RJ, (2010). RJ. Tuberculosis. Dalam: Loscalzo J, editor. Harrison’s Pulmonary and Critical Care Medicine. Ed. ke-1. 115–28 : McGraw-Hill Company


   


Archive Article

Cover Media Content

Volume : 21 / No. : 2 / Pub. : 2015-03
  1. Pneumatic tube on routine blood test and lactate dehydrogenase
  2. Detection in tetrazolium microplate assay culture methods from pulmonary tuberculosis suspected sputum
  3. High molecular weight adiponectin and vasculer thickness in diabetes type 2 related to fixed dose combination of glimepiride and metformin
  4. Neutrofil/lymphocyte ratio in carcinoma mammae
  5. Platelet aggregation and mean volume with metabolic syndrome in obesity
  6. Diagnosis of pulmonary tuberculosis based on frequency of sputum examination
  7. Genetic variation of hepatitis b virus polymerase gene from chronic hepatitis b infected patient with telbivudine therapy
  8. The 38 kda adhesin protein of mycobacterium tuberculosis and macrophage of the lung
  9. Bacterial pattern and patient’s age on procalcitonin in community and hospital acquired pneumonia
  10. Aspergillus glaucus group and penicillium sp in neurosurgery operating theater
  11. Diagnostic value of iga antivca epstein-barr antibody in nasophryngeal carcinoma
  12. Blood glucose test between hexokinase with glucose oxidase and glucose dehydrogenase methods in diabetes mellitus
  13. β-thalassemia trait using capillary electrophoresis
  14. Lipoprotein(a) in acute coronary syndrome
  15. D-dimer plasma levels in ishemic stroke
  16. Adrenomedullin’s in breast cancer with metastatic state
  17. Storage temperature for 24 hours of uric acid in urine
  18. Ebola virus disease
  19. Congenital malaria