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Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 17 / No. 2 / Published : 2010-11

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Original Article :

Diagnosis of dengue virus infection with iga anti dengue rapid tests

Author :

  1. Sri Kartika Sari*1
  2. Aryati*2
  1. Bagian Patologi Klinik, Fakultas Kedokteran, Universitas Airlangga, RSUD. Dr.Soetomo, Surabaya
  2. Bagian Patologi Klinik, Fakultas Kedokteran, Universitas Airlangga, RSUD. Dr.Soetomo, Surabaya

Abstract :

ABSTRACTDengue IgM, IgG Capture ELISAs and NS1 Ag ELISA become the most widely used serological methods for dengue diagnosis until now. Previous studies reported a possible use of IgA antibodies for dengue virus as a new serologic marker to make dengue infection active. In the present study, the performance of IgA anti-dengue rapid test as a new marker of dengue infection was assessed. In this study, the sera were obtained from 30 dengue virus infection patients and 30 non dengue virus infection patients. Thirty dengue paired sera were collected twice, at the time of hospital admission (acute) and at discharge (convalescent). All sera samples were characterized using dengue reference ELISAs (NS1 Ag, Dengue IgM and IgG capture ELISAs). The results of IgA anti-dengue rapid test were compared with the corresponding dengue reference tests. The sensitivity and specificity of IgA anti-dengue rapid test respectively were 78.3% (95% CI: 65.5–87.5%), and 73.3% (95% CI: 55,6–85,8%). Meanwhile, from acute sera, sensitivity of IgA anti-dengue rapid test was 83.3% (95% CI: 64.5–93.7), higher than IgM (73.3%, 95% CI: 53.8–87.0), IgG (66.7%, 95% CI: 47.1–82.1) and NS1 Ag ELISAs (60%, 95% CI: 40.7–76.8). Positive IgA anti-dengue rapid test results in acute sera was higher in the secondary (91%) than primary infection (57%). IgA anti-dengue rapid test can be considered as a new marker for dengue infection, because it gives a high sensitivity,especially in the acute phase and in the secondary infections as well.

Keyword :

IgA anti-dengue rapid test, dengue virus infection,


References :

  1. De Rivera IL, (2008). Humoral Immune Respone of Dengue Hemmoragic Fever . Honduras : Am Society of Tropical Medicine and Hygiene


   


Archive Article

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Volume : 17 / No. : 2 / Pub. : 2010-11
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  2. The creatinine level and potassium serum in patients with and without type ii diabetic
  3. Procalcitonin for the differentiation of bacterial and non bacterial infection
  4. Diagnosis of dengue virus infection with iga anti dengue rapid tests
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  6. Association between atypical depolarization on the cell-dyn 3200 and the presence of plasmodium spp in blood in the dr. soetomo hospital surabaya
  7. The correlation between thrombocyte and cd4 count in hiv/aids patients
  8. The chemotherapy effect in the activity of transaminase enzymes in breast cancer patients
  9. Pathogenesis and laboratory examination of primary myelofibrosis
  10. Star ()-flagged leukocytosis as indicator of interfering factor in automatic hematology analyzer
  11. (oral administration of mycobacterium tuberculosis 38-kilodalton adhesin protein increases macrophages and lymphocytes in intestinal balb/c mice)
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