UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 12 / No. 3 / Published : 2006-07

Related with : Scholar   Yahoo!   Bing

Original Article :

Diagnostic test of plasmodium malaria by immunochromatographic method compared to microscopic examination

Author :

  1. Ima Arum L*1
  2. Purwanto AP*2
  3. Arfi S*3
  4. Tetrawindu H,*4
  5. M Octora*5
  6. Mulyanto*6
  7. Surayah K*7
  8. Amanukarti*8
  1. Bagian Patologi Klinik FK UNDIP/RS Dr Kariadi Jl. Dr Sutomo 16-18 Semarang
  2. Bagian Patologi Klinik FK UNDIP/RS Dr Kariadi Jl. Dr Sutomo 16-18 Semarang
  3. Bagian Patologi Klinik FK UNDIP/RS Dr Kariadi Jl. Dr Sutomo 16-18 Semarang
  4. Bagian Patologi Klinik FK UNDIP/RS Dr Kariadi Jl. Dr Sutomo 16-18 Semarang
  5. Bagian Patologi Klinik FK UNDIP/RS Dr Kariadi Jl. Dr Sutomo 16-18 Semarang
  6. Bagian Patologi Klinik FK UNDIP/RS Dr Kariadi Jl. Dr Sutomo 16-18 Semarang
  7. Bagian Patologi Klinik FK UNDIP/RS Dr Kariadi Jl. Dr Sutomo 16-18 Semarang
  8. Bagian Patologi Klinik FK UNDIP/RS Dr Kariadi Jl. Dr Sutomo 16-18 Semarang

Abstract :

Microscopic examination is still a gold standard for malaria diagnostic tests. Immunochromatographic (IC) technique can be used as an alternative examination. The aim is to compare the identification value of immunochromatographic method diagnostic test to microscopic examination of malaria in laboratory examination. Cross sectional diagnostic study approach was applied to those with symptoms of: (1) fever (temperature > 38°C) or intermittent fever lasting for two days or more (2) cephalgia/myalgia, 604 samples were taken consecutively in January to July 2005 from primary health centres of Sukaraja and Keruak, as well as Clinic Nugraha at Lombok Timur district , province of Nusa Tenggara Barat . From these samples, there were 37 samples with P.vivax, 45 samples with P. falciparum, 5 samples with mixed infection and 517 samples without Plasmodium sp. From IC examination, results were reserved for 36 samples with P. vivax, 69 samples with P. falciparum, 5 samples with mixed infection and 503 samples without Plasmodium sp. Diagnostic test data analysis showed that the immunochromatographic test revealed 100% sensitivity, 96.7% specificity, 83.2% positive predictive value and 100% negative predictive value. It can be concluded that malaria IC test is reliable to be used as a routine malaria test.

Keyword :

plasmodium malaria, immunochromatographic, diagnostic test, microscopic examination,


References :

Moody,(2002) Rapid Diagnostic Tests of Malaria Parasites, Clin
Microbiol Rev 15. - : -

Kakkilaya,(2003) Rapid Diagnostic of Malaria, Lab. Medicine.
Aug , 2003 ; 8(34): 602-8. Avaliable from: www.malariasite.
com/malaria/rdts.htm. - : -

Warhust,(1996) Laboratory Diagnosis of Malaria, J. Clin. Pathol. - : -

Williams,(1996) Laboratory Diagnosis of Malaria, J. Clin. Pathol. - : -

Gilles,(1993) Diagnostic Methods in Malaria, In H.M. Gilles and
D.A. Warrell (ed), Essential malariology, 3rd ed. London : United Kingdom, P Edward Arnold

Anonymous,(2004) Central for Disease Control And Prevention (CDC),
Diagnosis Procedures for Blood Specimens, Atlanta, USA. - : -

Iqbal,(1999) Comparison of the Optimal test PCR for diagnosis of malaria in immigrants.J Clin Microbiol. - : -

Sher,(1999) Comparison of the Optimal test PCR for diagnosis of malaria in immigrants.J Clin Microbiol. - : -

Hira,(1999) Comparison of the Optimal test PCR fo



Archive Article

Cover Media Content

Volume : 12 / No. : 3 / Pub. : 2006-07
  1. Sepsis Diagnosis By Procalcitonin
  2. The Haematology Reference Value Of Healthy Adult People Based On Sysmex Xt-1800i
  3. Bacteria Pattern And Antibiotic Susceptibility Test Of Pregnant Women With Asymptomatic Bacteriuria)
  4. Bacterial Pattern And It’s Sensitivity In Patients Suffering From Urinary Tract Infection
  5. Diagnostic Test Of Plasmodium Malaria By Immunochromatographic Method Compared To Microscopic Examination
  6. Troponin T Value/ctnt Of Patients With Acute Coronary Syndrome)
  7. Plasma Cell Leukemia
  8. The Implementation Of Concept Mapping For Quality Management In A Clinical Laboratory
  9. The Analysis Of Urethral Stone Profile At The Clinical Pathology Laboratory

Information CPML