UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 17 / No. 2 / Published : 2010-11

Order : 2, and page :63 - 66

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Original Article :

Diazo test as a screening test of typhoid fever a practical approach

Author :

  1. J. Nugraha*1
  2. Meiti Muljanti*2
  1. Departemen Patologi Klinik Fakultas Kedokteran Universitas Airlangga
  2. Departemen Patologi Klinik Fakultas Kedokteran Universitas Airlangga

Abstract :

Typhoid fever represents an endemic acute infection with a high mortality, in this case a laboratory test is needed to establish the early diagnosis. For this study the researchers prefer urine diazo test which is a relatively easy, simple and inexpensive test. The aim of this study is to know whether the urine diazo test can be used for screening typhoid fever and whether there is a concordance with the Widal and TUBEX® TF serological tests. Forty patients aged 2–18 years, attending to the Jeremy Medical Service Clinic, Surabaya, suffering of fever more than 3 days were studied from June up to August 2010. Urine samples were tested by diazo test, while blood samples as comparison were tested by Widal and TUBEX® TF tests, and blood culture was carried out as the gold standard. The sensitivity and specificity of those tests were then recorded. From the 40 samples, 12 patients showed positive Salmonella typhi blood cultures, 26 diazo positive, 22 Widal positive and 14 TUBEX® TF positive. Of the 12 positive blood cultures, 10 (83%) diazo positive, 7 (58%) Widal positive and 9 (75%) TUBEX® TF positive were found. The sensitivity and specificity of diazo test was 83% and 43%, Widal test 58% and 46%, TUBEX® TF test 75% and 82%. It is shown that the Diazo test had a higher sensitivity value, while the TUBEX® TF test showed a higher level of specificity. In conclusion, so far it can be concluded that the Diazo test is quite reliable in aiding the diagnosis of typhoid fever and can be considered as a screening test for typhoid fever.

Keyword :

Typhoid fever, Diazo test, screening test,


References :

Handojo I,(2004) Immunoassai Untuk Demam Tifoid Surabaya : Airlangga University Press





Archive Article

Cover Media Content

Volume : 17 / No. : 2 / Pub. : 2010-11
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  2. The Creatinine Level And Potassium Serum In Patients With And Without Type Ii Diabetic
  3. Procalcitonin For The Differentiation Of Bacterial And Non Bacterial Infection
  4. Diagnosis Of Dengue Virus Infection With Iga Anti Dengue Rapid Tests
  5. The Platelet Aggregation Test As A Predictor Of Clinical Outcome In Acute Infarction Stroke
  6. Association Between Atypical Depolarization On The Cell-dyn 3200 And The Presence Of Plasmodium Spp In Blood In The Dr. Soetomo Hospital Surabaya
  7. The Correlation Between Thrombocyte And Cd4 Count In Hiv/aids Patients
  8. The Chemotherapy Effect In The Activity Of Transaminase Enzymes In Breast Cancer Patients
  9. Pathogenesis And Laboratory Examination Of Primary Myelofibrosis
  10. Star ()-flagged Leukocytosis As Indicator Of Interfering Factor In Automatic Hematology Analyzer
  11. (oral Administration Of Mycobacterium Tuberculosis 38-kilodalton Adhesin Protein Increases Macrophages And Lymphocytes In Intestinal Balb/c Mice)
  12. Diazo Test As A Screening Test Of Typhoid Fever A Practical Approach

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