Indonesian Journal of Clinical Pathology and Medical Laboratory
ISSN 0854-4263
Vol. 18 / No. 1 / Published : 2011-01
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Original Article :
Fitoestrogen dalam beberapa daun dan buah
Author :
- L. Maha putra*1
- Hening Laswati putra*2
- Lab Kebidanan, Dept. Vet. Reproduction,Airlangga University/Head Section of Genetic Resources Research East Java Province
- Head, Dept. Physical Medicine and Rehabilitation, Fac. Medicine /Dr. Soetomo General Hospital, Airlangga University
Abstract :
Phytoestrogen is molecularly almost similar with and acts the same as estrogen and is found a lot in several fruits and leaves grown either in tropical or subtropical countries. However, the quantity of molecular contents are not yet known exactly. In menopause and andropause, people need substitution of estrogens as a replacement therapy of sex hormone, due to the significant hormone decline and impacted disturbance of several organ functions and thus progressively causing severe organ dysfunctions. The objective of this study was to know the estrogen content by analyzing extractions of pegaga, green clover leaves, tomato and papaya fruit of which certain communites have overviewed contents of the leaves and fruit The samples which were collected for this purpose used 10 times replication in four different groups: the pegaga, green clover leaves, tomato and papaya fruit. All these groups were divided into two (2) subgroups based on the process or subspecies. All samples were made as an infusion 1:4) w/v), and then extracted after centrifugation 1000xg for 15mnts, with 1:5 petroleum ether) v/v). After it was evaporated, each extraction then was kept dry-frozen at -20oC until the analysis was performed. Solid phase Radioimmunoassay technique was used to identify the estrogen contents, up to a total of 80 samples The binding percentage of each sample was then interpolated on a logit-log paper to find out the real concentrations.14 The lowest estrogen level was found in fresh pegaga leaves extract (Mean+SD) was 47.9+5.5 pg/g, but in dry leaves extract the level was increased twice, about 96.1+11.2pg/g. Meanwhile the estrogen level in fresh green clover leaves extract was 538.0+30.5 pg/g, more than ten times higher compared to fresh pegaga level, but twice lower than the estrogen level compared to dry green clover leaves extract, which was 1068.0+97.2 pg/g. In the fruit group, the fibrin part of tomatoes had more or less the same estrogen content compared with Thai papaya subspecies, 1037.0+37.7 pg/g and 1175.0+67.7pg/g, respectively. On the other hand, it was noted that the inner part/ fibrin part of tomato had a higher estrogen level of four (4) times compared to the outer part which was 315.0+18.4 pg/g. While it was noted that local Java papaya besides being not so sweet, the estrogen level was also not as high or the same as that found in fresh green clover which is 530.1+50.7pg/g and 538.0+30.5pg/g. Based on this study so far, it can be concluded that semanggi/green clover, tomato and papaya could be suggested as a replacement therapy for certain people who are considered to have reduced estrogen content, except that pegaga leaves are not recommended. The last mentioned plant besides that it is difficult to obtain, its estrogen content is also very low. Fitoestrogen secara molekul hampir sama dan beraktifitas sama seperti estrogen dan ditemukan di jenis tanaman dan buah-buahan baik tropis maupun subtropis. Namun, jumlah kandungan molekulnya belum diketahui secara pasti. Perempuan yang mengalami menopause dan andropause diperlukan sulih hormon seks, karena umumnya kandungan hormon tersebut di mereka menurun secara bermakna. Hal tersebut dapat berdampak gangguan aktifitas fungsi organ serta dapat berkembang menjadi gangguan fungsi yang lebih berat. Tujuan penelitian ini adalah untuk mengetahui kandungan estrogen yang berasal dari sari daun pegaga, semanggi, buah tomat dan pepaya secara analisis, sehingga dampak terhadap masyarakat yang berpendapat pemahaman akan manfaat daun dan buah tersebut dapat dikukuhkan. Pengumpulan sampel dilakukan dengan cara cuplikan bertujuan (purposive sampling), masing-masing 20 kali ulangan di empat (4) kelompok, yaitu: kelompok daun: pegaga, dan semanggi, serta buah: tomat dan pepaya. Setiap kelompok dibagi menjadi sub-kelompok berdasarkan proses atau subspesies. Semua sampel dibuat infusa (1:4/w/v), setelah itu disarikan dengan petroleum eter (1:5 /v/v) yang sebelumnya dipusingkan 1000xg selama 15 menit. Selesai disarikan, sampel disimpan kering beku -20oC hingga penentuan kadar hormon dilakukan. Kadar estrogen ditentukan dengan metode Solid Phase Radioimmunoassay dengan jumlah keseluruhan 80 sampel, dan dengan memakai kertas logit-log untuk menggemariskan grafik baku, lalu dengan persentasi interpolasi ikatan setiap sampel didapatkan kadar hormon. Kadar hormon estrogen terendah ditemukan di sari daun pegaga segar dengan (rerata ± SD ) 47,9 ± 5,5 pg/g dan kadarnya meningkat dua kali lipat di sari daun pegaga kering yaitu 96,1±11,2 pg/g. Sementara kadar estrogen di sari semanggi segar adalah 538,0±30,5 pg/g dibandingkan dengan sari pegaga segar. Kandungan ini sepuluh kali lipat lebih tinggi, tetapi kurang lebih dua kali lebih rendah kadar estrogennya bila dibandingkan dengan sari daun semanggi kering yaitu 1068,0±97,2 pg/g. Di kelompok buah-buahan bagian lendir tomat tampak kurang lebih sama kandungan estrogennya dengan jenis pepaya Thailand yaitu masing-masing 1037,0±37,7 pg/g dan 1175,0±67,7 pg/g. Sebagai catatan bahwa bagian dalam atau bagian yang berlendir buah tomat memiliki kadar estrogen kurang lebih empat kali lipat lebih tinggi dibandingkan dengan bagian luar yaitu 315,0±18,4 pg/g. Di lain pihak pepaya Jawa lokal di samping tidak manis dan kandungan estrogennya tidak begitu tinggi atau sama dengan yang didapatkan di daun semanggi segar masing-masing yaitu 530,1±50,7 pg/g dan 538,0±30,5 pg/g. Daun semanggi, buah tomat, dan pepaya dapat disarankan sebagai sulihan pengobatan hormon untuk orang tertentu yang memerlukan, tetapi daun pegaga tidak dianjurkan karena sulit didapatkan, selain itu kandungan hormon estrogennya juga rendah.
Keyword :
Phytoestrogen, pegaga, green clover, tomato and papaya ,
References :
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Archive Article
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 Volume : 18 / No. : 1 / Pub. : 2011-01 |
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