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Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 19 / No. 2 / Published : 2013-01

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Original Article :

Gandarusa (justicia gendarussa burm.f.) water and expression of hyaluronidase gene by pcr analysis

Author :

  1. Sri Lestari Utami*1
  2. Didik P. Restanto*2
  3. Bambang Prajogo EW*3
  1. Bagian Biomedik Fakultas Kedokteran-Universitas Wijaya Kusuma Surabaya
  2. Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Jember
  3. Bagian Ilmu Bahan Alam Fakultas Farmasi Universitas Airlangga

Abstract :

Gandarusa (J. gendarussa Burm. f.) is an etnomedicine which is used as a male contraceptive alternative, known to inhibit the enzyme function of spermatozoa hyaluronidase during fertilization. One of the ideal contraceptive conditions is the safetyness (among others non mutagenic), requiring a long research during the verification process. The early research is the gene expression of hyaluronidase mice (M.musculus L.) testis given water fraction of the gandarusa with PCR analysis. The total RNA was isolated from the normal mice testis (as the negative control or no treatment group) and the mice testis from the treatment groups. The treatment groups consisted of group I and II treated with water fraction of the gandarusa 15 mg/20 gr BW and 7.5 mg/20 gr BW, subsequently, the positive control group was also given  hesperidins 1 mg/20 gr BW once a day per oral during the 1.5 times of spermatogenesis cycle (for 55 days). The results of the study showed that (1) the cDNA fragment confirmed as the gene of hyaluronidase mice testis (with 710 bp length of nucleotide) passed through RT-PCR at the total RNA negative control group, sequenced, isolated and alignment in the NCBI gene bank. (2) the same cDNA fragment gene of hyaluronidase mice testis (from the negative control group) did not transcript in the treatment I and positive control groups (where there is no band), but this gene will be transcripted in the treatment II group (where the band is emerged).     Gandarusa (J. gendarussa Burm. f.) sebagai etnomedisin merupakan pilihan kontrasepsi dari laki-laki, yaitu bahan yang diketahui dapat menghambat fungsi enzim hyaluronidase di kepala akrosom spermatozoa selama pembuahan. Di antara syarat kontrasepsi yang baik adalah bahan tersebut aman penggunaannya (di antaranya adalah non mutagenik), dan untuk itu selama pembuktiannya memerlukan penelitian yang lama. Penelitian awalnya adalah mengenai ekspresi gen hyaluronidase testis mencit (Mus musculus L.) yang diberi fraksi air gandarusa menggunakan analisis PCR. RNA lengkap diisolasi dari testis mencit sehat (sebagai kelompok pembanding negatif atau tanpa perlakuan) dan kelompok perlakuan. Kelompok perlakuan : kelompok I dan II diberi fraksi air gandarusa 15 mg/20 gr BB dan 7,5 mg/ 20 gr BB serta kelompok pembanding positif diberi hesperidin  mg/ 20 gr BB  sebanyak satu kali sehari lewat rongga mulut selama 1,5 kali siklus pembentukan spermatogenesis (55 hari). Hasil kajian ini adalah: Fragmen cDNA yang dapat dipastikan sebagai gen hyaluronidase testis mencit (dengan panjang nukleotida 710 bp), yang didapatkan melalui RT-PCR di RNA lengkap kelompok pembanding negatif, diurutkan, diisolasi dan dijajarkan menggunakan bahan dari Bank gen NCBI; Fragmen cDNA dari gen hyaluronidase kelompok pembanding negatif tersebut tidak ditranskripsikan di kelompok perlakuan I dan pembanding positif (tidak ada pita), tetapi ditranskripsikan di kelompok perlakuan II (terlihat pita). 

Keyword :

Male contraception, gandarusa, hyaluronidase gene, PCR analysis,


References :

  1. 1. Hatcher RA, Trussel J, Nelson AL, Cates WJ, Stewart FH, Kowal , (2009). Contraceptice Technology. USA : Contraceptive Technology Communications Inc
  2. Hatcher RA, Stewart F, Trussell J et. al, (1990). Contraceptive Technology 1990-1992. New York : Irvington Publishers
  3. Beck M, (2012). “Honey, Its Your Turn …”, . HealthJournal@wsj.com (accesed March 30, 2012) : The Wall Street Journal
  4. Comhaire FH, (1994). Male contraception: hormonal, mechanical and other. USA : Hum. Reprod
  5. 5. Engelmann VH, Schramek P, Tomamichel G, (1990). Vasectomy reversal in central Europe : result of questionnare of urologists in Austria, Germany and Switzerland. USA : Journal of Urology


   


Archive Article

Cover Media Content

Volume : 19 / No. : 2 / Pub. : 2013-01
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  2. Gandarusa (justicia gendarussa burm.f.) water and expression of hyaluronidase gene by pcr analysis
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