UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 18 / No. 1 / Published : 2011-01

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Original Article :

Identification of cryptosporidiosis in paediatric hiv-infected patients with chronic diarrhoea at paediatric gastro

Author :

  1. Jusak Nugraha*1
  2. Febtarini Rahmawati*2
  3. D Husada*3
  1. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya
  2. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya
  3. Bagian Anak, Fakultas Kedokteran, Universitas Airlangga/RS. Dr. Soetomo Surabaya

Abstract :

  Based on the results of overseas researchers, Cryptosporidiosis occurs in immunosuppressive cases with chronic diarrhoea. In this study the researchers would like to know exactly whether that Cryptosporidiosis occurs also in paediatric HIV patients. The latest data show that the incidence of opportunistic infection is characterized by persistent diarrhoea and severe malnutrition as a complication of the paediatric HIV-infected patients is increasing. The objects of the research were fifteen paediatric HIV-infected patients which treated at the Paediatric Gastro Ward of Dr. Soetomo Hospital Surabaya due to persistent diarrhoea. Paediatric patients were less than five years old, suffered persistent diarrhoea more than two weeks with severe malnutrition. Stool specimens were transported using 10% formalin. The stool suspension was filtered, and distilled water was added followed by centrifugation (sedimentation method). The precipitate material was placed on a glass object and dried, and then fixed by methanol and stained with Acid Fast Staining and trichrome staining. The protozoa Cryptosporidium spp. was observed under a binocular microscope with 100X magnification (immersion oil) objective. The result was confirmed as positive if a red spherical or oval formation of oocyste of 4-6 micron appeared. Sixty percent of the 15 paediatric HIV-infected patients with chronic diarrhoea showed positive cryptosporidiosis. Cryptosporidiosis is one of the opportunistic infections resulting in chronic diarrhoea in paediatric HIV-infected patients. The results of the present research indicate that the enteric parasite Cryptosporidium spp. was the main cause of persistent diarrhoea in paediatric HIV-infected patients.   Berdasarkan hasil telitian sebelumnya, Kriptosporidiosis terjadi di pasien dengan keadaan kekebalan yang menurun disertai diare kronik. Para peneliti ingin mengetahui apakah hal tersebut juga terjadi di pasien anak. Didasari data terbaru menunjukkan ada peningkatan kejadian infeksi oportunistik dengan malagizi berat di pasien HIV anak. Sebagai subjek penelitian ialah penderita HIV anak dengan diare menetap yang dirawat di Ruang Gastro Anak RSUD Dr. Soetomo Surabaya. Usia penderita adalah di bawah lima tahun dan mengalami diare lebih dari dua minggu disertai malagizi berat. Pengumpulan bahan tinja menggunakan 3 mL formalin 10%, selanjutnya diolah di laboratorium dengan cara mengendapkan (metode sedimentasi). Bahan tinja tersebut disaring, lalu ditambah air suling dan pusingkan/disentrifus (3 ― 4 kali) hingga bahan menjernih. Endapan kemudian diletakkan di atas gelas obyek dan dikeringkan. Gelas slide yang telah kering direkatkan (difiksasi) dengan metanol dan dicat menggunakan cat tahan asam dan Trichrome. Di bawah mikroskop dengan pembesaran lensa obyektif 100X yang dibubuhi minyak celup (imersi) didapatkan protozoa Cryptosporidium spp. Bentuk parasit lonjong atau membulat (sferikal) kemerahan dengan ukuran 4 – 6 mikron. Enam puluh persen dari 15 penderita HIV anak pengidap diare kronik ditemukan positif Cryptosporidium spp. Kriptosporidiosis adalah salah satu infeksi oportunistik yang ditandai dengan gejala diare kronik. Cryptosporidium spp. merupakan penyebab diare kronik terbanyak di penderita HIV anak.

Keyword :

Cryptosporidiosis, chronic diarrhoea,


References :

Mor Siobhan M, Tzipori Saul,(2008) Cryptosporidiosis in Children in Sub-Saharan Africa Afrika : A Lingering Challenge

Amadi Beatrice, Kelly Paul,(2001) Intestinal and Systemic Infection, HIV, and Mortality in Zambian Children with Persisten Diarrhea and Malnutrition USA : Journal of Pediatric Gastroenterology and Nutrition

Brandonisio O, Marangi A,(1996) Prevalence of Cryptosporidium in children with enteritis in Southern Italy Eropa : European Journal of Epidemiology

Nchito Mbiko, Kelly Paul,(1998) Cryptosporidiosis in Urban Zambian Children: An Analysis of risk factors USA : . The American Society of Tropical Medicine and Hygiene

Cegielski J P, R Ynes,(1998) Cryptosporidium, Enterocytozoon, and Cyclospora Infection in Pediatric Patients with Diarrhea in Tanzania Tanzania : Cryptosporidium, Enterocytozoon, and Cyclospora Infection in Pediatric Patients with Diarrhea in Tanzania





Archive Article

Cover Media Content

Volume : 18 / No. : 1 / Pub. : 2011-01
  1. Aerob Microbes Pattern And Antimicrobial Sensitivity Of Diabetic Foot Ulcer
  2. Plasma Levels Of Interleukin 10 (il-10) In Malaria And Anaemia
  3. Identification Of Cryptosporidiosis In Paediatric Hiv-infected Patients With Chronic Diarrhoea At Paediatric Gastro
  4. Immunoglobulin A In Dengue Hemorrhagic Fever
  5. (comparison Of Determination For Thyroxine With Enzyme Linked Immunofluorescent Assay (elfa) And Enzyme Linked Immunosorbant Assay (elisa)}
  6. Plasma Interleukin-10 And Cd4+ Lymphocyte-t In Hiv Infected Patients
  7. Detecting Fluoroquinolone Resistance Of Salmonella Sp Using Nalidixic Acid Succeptibility Test
  8. Phyllanthus Niruri L The Effects Of Extract On Cellular Immunity Mice
  9. Fitoestrogen Dalam Beberapa Daun Dan Buah
  10. Diagnostic Test Nt Pro Natriuretic Peptide (ntprobnp) On Congestive Heart Failure
  11. Human Immunodeficiency Virus (hiv) Infection In Babies And Children
  12. Decompensated Cirrhosis Hepatic In Children
  13. Human Resources Management In The Clinical Laboratory

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