UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 21 / No. 1 / Published : 2014-01

Order : 9, and page :40 - 44

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Original Article :

Immature platelet fraction in dengue fever and dengue hemorrhagic fever

Author :

  1. Izzuki Muhashonah*1
  2. Juli Soemarsono*2
  3. Puspa Wardhani*3
  4. Aryati*4
  1. Departemen/Instalasi Patologi Klinik Fakultas Kedokteran Universitas Airlangga/RSUD Dr. Soetomo Surabaya
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran
  4. Dosen Fakultas Kedokteran

Abstract :

Thrombocytopenia is a hematological abnormality found in the majority of Dengue Virus Infection cases with manifestations such as Dengue Fever (DF) and Dengue Hemorrhagic Fever (DHF). Bone marrow response to the decrease in platelets is by increasing thrombopoiesis which can be identified by Immature Platelet Fraction (IPF) examination as an indirect indicator of bone marrow response to thrombocytopenia. The examination of IPF in venous blood was performed on 29 subjects who met the 1997 WHO criteria, carried out from January until August 2012. The EDTA blood samples were examined twice, on the day of their admittance and two days later, based on a flowcytometry principle using Sysmex XE-2100. The IPF was derived from the immature platelet ratio against the total number of platelets (IPF %). The test results were statistically analyzed by using SPSS 20. It was found, that IPF in DHF compared between the first and the third day of their admittance was statistically significantly different with p = 0.033 compared to DF with p = 0.444. The Pearson’s correlation showed an inverse correlation between IPF and platelets with r = -0.675 and p = 0.01. The statistical analysis revealed a significant difference in IPF between moderate- and mild-thrombocytopenia on the first and third day of their admittance with p = 0.014 and 0.001, respectively. Based on this study it can be concluded that IPF can be used to indicate the bone marrow response in both DF and DHF related to thrombocytopenia. Respons sumsum tulang terhadap penurunan trombosit adalah dengan meningkatkan trombopoisis dan dapat diketahui dengan pemeriksaan Immature Platelet Fraction (IPF). Penelitian ini untuk mengetahui IPF sebagai penunjuk respons sumsum tulang terhadap trombositopenia dengan menilainya di infeksi virus dengue (IVD). Pemeriksaan IPF darah vena dilakukan di subjek yang memenuhi patokan World Health Organization (WHO) 1997 dari bulan Januari sampai Agustus 2012. Darah EDTA diperiksa pada hari pertama dan ketiga Masuk Rumah Sakit (MRS) dengan Sysmex XE-2100 menggunakan asas flowcytometry. Immature Platelet Fraction didapat dari angka banding trombosit belum dewasa (immature) terhadap jumlah keseluruhannya (IPF %). Hasil memeriksa dianalisis secara statistik menggunakan SPSS 20. Immature Platelet Fraction pada hari pertama Demam Berdarah Dengue (DBD) dalam hal ini diketahui, dibandingkan dengan yang ketiga MRS berbeda bermakna secara statistik dengan p = 0,033 dan jika diperbandingkan dengan Demam Dengue (DD) (p= 0,444). Kenasaban Pearson menunjukkan keadaan yang terbalik antara IPF dan trombosit dengan r=-0,675 dan p=0,01. Analisis statistik menunjukkan IPF di trombositopenia tingkat sedang dan berbeda bermakna dibandingkan yang ringan pada hari pertama dan ketiga MRS dengan p berturut-turut 0,014 dan 0,001. Berdasarkan kajian ini dapat disimpulkan bahwa IPF dapat dipakai sebagai penunjuk respons sumsum tulang di DD dan DBD terhadap keberadaan trombositopenia.

Keyword :

Immature platelet fraction, dengue fever, dengue hemorrhagic fever, thrombocytopenia,


References :

Hadinegoro SR., Soegijanto S., Wuryadi S., Soeroso TH.,(2000) Tata Laksana Demam Dengue/Demam Berdarah Dengue pada Anak Hal. 81-91 : Fakultas Kedokteran Universitas Indonesia

Aryati,(2006) Aspek Laboratorium DBD. In: Soegijanto S, editor. Demam Berdarah Dengue. Hal. 117-32 : Airlangga University Press





Archive Article

Cover Media Content

Volume : 21 / No. : 1 / Pub. : 2014-01
  1. The Level Of Interleukin-6 Plasma In Diabetes Mellitus Patients With And Without Diabetic Retinopathy
  2. Correlation Plasma Fibrinogen With Intima-media Thickness Of Carotid Artery In Diabetes Mellitus
  3. Matrix Metalloproteinase-2 In Breast Cancer Metastastis
  4. Potassium In Multidrug Resistance Tuberculosis With Kanamycin
  5. Safe Blood And Voluntary Non-remunerated Blood Donors
  6. Mean Platelet Volume In Diabetes Mellitus
  7. Ratio Of Urinary Uric Acid Levels And Serum Uric Acid In Type 2 Diabetes Mellitus
  8. Reticulocyte Hemoglobin Level Of Absolute Iron Deficiency Anemia And Nonabsolute Iron Deficiency Anemia In End State Renal Disease Undergoing Maintenance Hemodialysis
  9. Immature Platelet Fraction In Dengue Fever And Dengue Hemorrhagic Fever
  10. Cryptococcal Antigen Of Acquired Immune Deficiency Syndrome With Lateral Flow Assay And Cryptococcus Antigen Latex Agglutination System
  11. T-cd4+ And Lipid Profile In Hiv)
  12. Liver Function Parameters Based On Degree Of Liver Fibrosis In Chronic Liver Disease
  13. Brain Derived Neurotrophic Factor (bdnf) As A Prognostic Factor In Severe Head Injury
  14. Hpv Genotype And Hpv Infection Pattern Related To The Histopathological Type Of Cervical Cancer)
  15. Glut 4 In Adipose Tissue
  16. The Diagnostic Value Of Anti Dengue Iga And Anti Dengue Igm/igg In Dengue Virus Infection)
  17. Vitamin D Deficiency And Diseases
  18. Lineage Switch From Acute Lymphoblastic Leukemia To Acute Myelomonocytic Leukemia At A 26 Years Old Woman
  19. The Role For Clinical Pathologist In Hospital Accreditation

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