UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 18 / No. 1 / Published : 2011-01

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Original Article :

Immunoglobulin a in dengue hemorrhagic fever

Author :

  1. Iwan Joseph*1
  2. Uleng Bahrun*2
  3. Idham Jaya Ganda*3
  4. M Arif*4
  1. Bag PK FK-UNHAS Makassar
  2. Bag PK FK-UNHAS Makassar
  3. Bag PK FK-UNHAS Makassar
  4. Bag PK FK-UNHAS Makassar

Abstract :

  Recently several serology tests have been used to detect IgM and IgG antidengue, and NS1 antigen. On the last study was launched a new diagnose test using immunochromatography method to detect the specific antidengue IgA antibody. The aim of this study was to evaluate the IgA antidengue performance using immunochromatography method in Dengue Hemorrhagic Fever patients. The study was carried out during March up to July 2010 by a cross sectional method. The samples taken were 1―14 year‘s old patients from the Infectious Tropical Sub Division of the Department of Paediatric, Dr. Wahidin Sudirohusodo Hospital, Makassar. The samples were diagnosed Dengue Hemorrhagic Fever based on the criteria of WHO 1997 and they also subjected to IgM and IgG serology tests as well. The test for IgA is using immunochromatography method. The data was analyzed by Spearman Correlation Test. From the total 40 samples, which consist of 16 (40%) male and 24 women (60%), generally were taken on the fourth day until the sixth day of onset. There were more IgA anti Dengue detected rather than IgM on the secondary infection. The researchers found moderate correlation among the IgA test with IgM (+), IgG (+), or both positive.  Based on this study so far it can be concluded that the Dengue IgA test can be used to detect the primary or secondary dengue virus infection.     Saat ini banyak digunakan pemeriksaan serologis yaitu untuk menemukan antibodi IgM, IgG antidengue dan antigen Dengue NS1. Telitian terbaru menghasilkan metode diagnosis baru menggunakan metode imunokromatografi untuk menemukan antibodi khas IgA terhadap virus dengue. Penelitian ini dilakukan untuk menilai IgA antidengue terhadap IgM dan IgG antidengue dengan metode imunokromatografi bagi pasien DBD berdasarkan patokan WHO. Penelitian dilakukan selama masa waktu Maret―Juli 2010 secara potong silang (cross sectional). Sampel adalah semua penderita berumur antara 1―14 tahun yang dirawat di Sub Divisi Infeksi Tropis Bagian Ilmu Kesehatan Anak RS Dr. Wahidin Sudirohusodo Makassar, yang didiagnosis DBD berdasarkan patokan WHO 1997 dan diuji secara uji cepat serologis IgM dan IgG. Pemeriksaan IgA menggunakan metode imunokromatografi. Data dianalisis dengan Uji kenasaban Spearman. Dari 40 sampel, 16 orang (40%) adalah laki-laki dan 24 orang (60%) adalah perempuan, yang umumnya sampel diambil pada hari ke-4 sampai ke-6 demam. Anti Dengue IgA lebih banyak tertemukan daripada IgM di infeksi sekunder. Terdapat kenasaban positif dengan kekuatan kenasaban sedang antara hasil uji IgA terhadap IgM(+), IgG(+), atau keduanya positif. Didasari telitian ini dapat disimpulkan bahwa uji dengue IgA dapat digunakan untuk menemukan infeksi virus dengue, baik infeksi primer maupun sekunder.

Keyword :

IgA antidengue, IgM antidengue, IgG antidengue, DHF,


References :

Suhendro, Nainggolan L,(2007) Demam Berdarah Dengue Jakarta : Pusat Penerbitan FKUI

Djunaedi D,(2006) Demam Berdarah Malang : UPT Penerbitan UMM

Massi M.N, Sabran A.A,(2009) Teknik Identifikasi Serotipe Virus Dengue dengan Uji Reverse Transcription Polymersase Chain Reaction (RT-PCR) USA : http://med.unhas.ac.id/DataJurnal/tahun2006.pdf

Sarker S,(2009) What and How much we know about Dengue fever? India : http://www.scribd.com/doc/5000178/Dengue-Fever?autodown=pdf

Sugeng S,(2009) Patogenesa dan Perubahan Patofisiologi Infeksi Virus Dengue Surabaya : http://www.pediatrik.com/buletin/20060220-8ma2gi- buletin.pdf





Archive Article

Cover Media Content

Volume : 18 / No. : 1 / Pub. : 2011-01
  1. Aerob Microbes Pattern And Antimicrobial Sensitivity Of Diabetic Foot Ulcer
  2. Plasma Levels Of Interleukin 10 (il-10) In Malaria And Anaemia
  3. Identification Of Cryptosporidiosis In Paediatric Hiv-infected Patients With Chronic Diarrhoea At Paediatric Gastro
  4. Immunoglobulin A In Dengue Hemorrhagic Fever
  5. (comparison Of Determination For Thyroxine With Enzyme Linked Immunofluorescent Assay (elfa) And Enzyme Linked Immunosorbant Assay (elisa)}
  6. Plasma Interleukin-10 And Cd4+ Lymphocyte-t In Hiv Infected Patients
  7. Detecting Fluoroquinolone Resistance Of Salmonella Sp Using Nalidixic Acid Succeptibility Test
  8. Phyllanthus Niruri L The Effects Of Extract On Cellular Immunity Mice
  9. Fitoestrogen Dalam Beberapa Daun Dan Buah
  10. Diagnostic Test Nt Pro Natriuretic Peptide (ntprobnp) On Congestive Heart Failure
  11. Human Immunodeficiency Virus (hiv) Infection In Babies And Children
  12. Decompensated Cirrhosis Hepatic In Children
  13. Human Resources Management In The Clinical Laboratory

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