UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 19 / No. 2 / Published : 2013-01

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Original Article :

Katekin from green tea leaves (camellia sinensis) to malondialdehyde (mda) and super oxide dismutase (sod)

Author :

  1. Sukina B*1
  2. Gwenny I.P *2
  3. Suhartati*3
  4. Harianto N*4
  1. Jurusan Gizi Fakultas Politeknik Kesehatan Kendari
  2. Departemen Biokimia Kedokteran Fakultas Kedokteran, Universitas Airlangga Surabaya
  3. Departemen Biokimia Kedokteran Fakultas Kedokteran, Universitas Airlangga Surabaya
  4. Departemen Biokimia Kedokteran Fakultas Kedokteran, Universitas Airlangga Surabaya

Abstract :

       Recent studies have shown that Plumbum could disrupt tissue prooxidant/antioxidant balance. The green tea leaves (Camellia sinensis) is an antioxidant scavenger of free radicals and chelator of heavy metals. This study was designed to know and investigate the efficacy of Katekin from green tea leaves malondialdehid (MDA) and superoxide dismutase (SOD) activity in erythrocytes caused by oral Pb administration in Wistar rats. Thirty adult male rats were divided into six (6) groups ( each groups : 5 rats):  K+ group (oral 33.75 mg/bw Katekin), K group as a normal control, K- group (oral 15.82 mg/day lead acetate), P1 group (oral 22.5 mg/bw Katekin and 15.82 mg/day lead acetate, P2 group (oral 33.75 mg/bw Katekin and 15.82 mg/day lead acetate for), and P3 group (oral 45 mg/bw/Katekin and 15.82 mg/day lead acetate) for 4 weeks. The results showed that Pb exposure induced the raised of MDA levels and decrease SOD activity in erythrocytes of rats. The administration of Katekin from green tea leaves significantly reduced MDA levels and increased SOD activity in Pb exposed erythrocytes  of rats. The optimal dose of Katekin from green tea leaves as a scavenger of free radicals and chelator of heavy metals was 22.5 mg/bw/day (P1). These results indicated that the administration of Katekin from green tea leaves may have an important role in modulating oxidative stress in Pb exposed erythrocytes, but at a higher concentration of Katekin from green tea leaves showed prooxidant activity.      Telitian yang sudah ada menunjukkan bahwa Plumbum dapat mengganggu keseimbangan prooksidan /oksidan di jaringan. Katekin daun teh hijau (Camelia sinensis) berperan sebagai antioksidan dan mengikat logam berat. Pada penelitian ini digunakan 30  ekor subjek penelitian tikus putih jantan galur Wistar (Rattus norvegicus) yang dibagi menjadi enam (6) kelompok (setiap kelompok terdiri dari lima (5) ekor). Kelompok K(+)  (diberi air suling dan Katekin 33,75 mg/hari/oral); kelompok K sebagai pembanding normal (diberi air suling); kelompok K(-) (diberi Pb asetat 15,82 mg/hari/oral); kelompok perlakuan P1 (diberi Pb asetat 15,82 mg/hari/oral dan Katekin 22,5 mg/hari/oral), kelompok perlakuan P2 (diberi Pb asetat 15,82 mg/hari/oral dan Katekin 33,75 mg/hari/oral selama empat/4 minggu), dan kelompok perlakuan P3 (diberi Pb asetat 15,82 mg/hari/oral dan Katekin 45 mg/hari/oral) selama empat (4) minggu. Hasil telitian menunjukkan bahwa pemberian Pb asetat dapat meningkatkan kadar malondialdehid (MDA|) dan menurunkan aktivitas (superoxide dismutase) SOD di eritrosit tikus jantan galur Wistar. Pemberian Katekin daun teh hijau memiliki manfaat dalam mengikat ion logam peralihan serta mencegah peningkatan kadar MDA dan penurunan aktivitas enzim SOD di eritrosit tikus putih jantan galur Wistar yang dipajan Pb, tetapi di kepekatan yang lebih tinggi Katekin daun teh hijau memperlihatkan aktivitas sebagai prooksidan.

Keyword :

Katekin from green tea leaves, malondialdehyde, super oxide dismutase, acetate lead,


References :

Anggarini,(2007) Toxicological profile for lead us department of health and human services USA : Atlanta Georgia

Mishra KP, Chauhan UK, Naik S,(2006) Effect of lead exposure on serum immunoglobulins and reactive nitrogen and oxygen intermediate USA : Human & Experimental Toxicology

Patrick L,(2006) Lead toxicity part II: the role of free radical damage and the use of antioxidants in the pathology and treatment of lead toxicity USA : Alternative Medicine Review

Suryohudoyo P,(2000) Kapita selekta: ilmu kedokteran molekuler Surabaya : CV Sagung Seto

Chen L, Yang X, Jiao H, Zhao B,(2002) Tea catechins protect against lead-induced ros formation mitochondrial dysfunction and calcium dysregulation in pc12 Cells USA : Chem Res Toxicol





Archive Article

Cover Media Content

Volume : 19 / No. : 2 / Pub. : 2013-01
  1. Clinical Manifestion Sepsis And Nitric Oxide Level On Mice Induced By Lipopolysaccharide
  2. Gandarusa (justicia Gendarussa Burm.f.) Water And Expression Of Hyaluronidase Gene By Pcr Analysis
  3. Proteinuria In Stroke With And Without Diabetic
  4. The Stewart’s Approach In Blood Ph Underlying Metabolic Acidosis
  5. Microbes And Antimicrobial Sensitivity In Open Fracture
  6. Katekin From Green Tea Leaves (camellia Sinensis) To Malondialdehyde (mda) And Super Oxide Dismutase (sod)
  7. Procalcitonin And Interleukin-6 In Sepsis Systemic Inflammatory Response Syndrome (sirs)
  8. Gram Negative And Gram Positive Aerobic Bacteria Identification Using Conventional And Automatic Method
  9. Immature Platelet Fraction (ipf) And Thrombopoietin In Liver Cirrhosis
  10. Eosinopenia And Procalcitonin In Sepsis
  11. C-x-c Receptor 4 (cxcr4) In Metastasis Of Breast Cancer
  12. Hairy Cell Leukaemia

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