UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 21 / No. 1 / Published : 2014-01

Order : 3, and page :11 - 15

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Original Article :

Matrix metalloproteinase-2 in breast cancer metastastis

Author :

  1. Besse Rosmiati*1
  2. Uleng Bahrun*2
  3. Ruland DN Pakasi*3
  1. Ilmu Patologi Klinik FK UNHAS Makassar.
  2. Ilmu Patologi Klinik FK UNHAS Makassar
  3. Ilmu Patologi Klinik FK UNHAS Makassar

Abstract :

Matrix Metalloproteinase-2 is a family of extra cellular matrix degrading proteinases. Study results on the role of MMP-2 in breast cancer progression and metastasis are still controversial. The aim of this study was to know the MMP-2 level by analyzing in metastatic and non metastatic breast cancer patients by using a cross sectional design, it was carried out in the Wahidin Sudirohusodo, Ibnu Sina, Labuang Baji Hospitals and at the Research Unit of UNHAS Makassar from April 2012 until June 2012. The 56 breast cancer patients were divided into two groups: metastatic and non metastatic based on their thorax photo, ultrasonography and/or bone scan results. They were also divided into early and advanced stage, based on their TNM staging. The matrix metalloproteinase-2 level was determined by ELISA method. The result of this study showed that the mean levels of MMP-2 in metastatic and non metastatic breast cancer were 20.18 ng/mL and 17.14 ng/mL, respectively. The independent sample T test showed there was a significant difference (p=0.018) in MMP-2 levels between metastatic and non metastatic breast cancer. The mean level of MMP-2 in early and advanced stages was 17.10 ng/mL and 18.31 ng/mL, respectively, the independent sample T test showed no significant difference of MMP-2 level between both stages. One way ANOVA test showed no significant difference of MMP-2 level based on tumour size and regional lymph node infiltration. The MMP-2 contributed to the metastasis in breast cancer patients. The matrix metalloproteinase-2 level in metastatic was higher than in the non metastatic breast cancer, so this condition could be used as a biomarker to predict the metastasis of breast cancer. Matriks metaloproteinase-2 (MMP-2) adalah famili enzim proteinase yang dapat mendegradasi komponen matriks ekstraselular. Hasil meneliti peran MMP-2 di kemajuan dan metastasis karsinoma payudara masih diperselisihkan. Tujuan penelitian ini adalah untuk mengetahui kadar MMP-2 di pasien karsinoma payudara dengan menganalisis kelompok yang metastasis dan yang bukan. Penelitian ini menggunakan rancangan potong silang dilakukan di RSUP Dr. Wahidin Sudirohusodo, RS Ibnu Sina, RS Labuang Baji Makassar dan Unit Penelitian UNHAS mulai bulan April 2012 sampai bulan Juni 2012. Sebanyak 56 pasien karsinoma payudara dibagi menjadi dua kelompok yaitu yang bermetastasis dan nonmetastasis berdasarkan hasil memeriksa foto dada, ultrasonografi dan atau bone scan. Subjek juga dikelompokkan menjadi tingkat dini dan lanjut berdasarkan TNM. Kadar serum MMP-2 diukur dengan metode Enzyme-Linked Immunosorbent Assay (ELISA). Hasil meneliti ini menunjukkan rerata kadar MMP-2 di karsinoma payudara yang metastasis dan nonmetastasis masing-masing adalah 20,18 ng/ mL dan 17,14 ng/mL. Independent sample T test menunjukkan ada perbedaan bermakna kadar MMP-2 antara metastasis dan yang nonmetastasis (p=0,018). Rerata kadar MMP-2 pada tingkat dini dan lanjut adalah masingmasing 17,10 ng/mL dan 18,31 ng/mL, independent sample T test menunjukkan tidak ada perbedaan bermakna antara kedua tingkatan tersebut. One way anova test menunjukkan tidak ada perbedaan bermakna kadar MMP-2 berdasarkan ukuran tumor dan infiltrasi ke kelenjar wilayah getah bening MMP-2 berperan terhadap metastasis di pasien. Kadar MMP-2 lebih tinggi di metastasis dibandingkan dengan nonmetastasis, sehingga dapat digunakan sebagai biomarker untuk meramalkan metastasis karsinoma payudara.

Keyword :

Matrix metalloproteinase-2, breast cancer, metastasis,


References :

Tjindarbumi D,(2000) Deteksi Dini Kanker Payudara dan Penanggulangannya In: Deteksi Dini Kanker hal 32-43 : Balai Penerbit FKUI

Jemal A, Bray F, Melissa M, Ferlay J, Ward E, Forman D,(2011) Global Cancer Statistics Hal 69-90 : CA Cancer J Clin





Archive Article

Cover Media Content

Volume : 21 / No. : 1 / Pub. : 2014-01
  1. The Level Of Interleukin-6 Plasma In Diabetes Mellitus Patients With And Without Diabetic Retinopathy
  2. Correlation Plasma Fibrinogen With Intima-media Thickness Of Carotid Artery In Diabetes Mellitus
  3. Matrix Metalloproteinase-2 In Breast Cancer Metastastis
  4. Potassium In Multidrug Resistance Tuberculosis With Kanamycin
  5. Safe Blood And Voluntary Non-remunerated Blood Donors
  6. Mean Platelet Volume In Diabetes Mellitus
  7. Ratio Of Urinary Uric Acid Levels And Serum Uric Acid In Type 2 Diabetes Mellitus
  8. Reticulocyte Hemoglobin Level Of Absolute Iron Deficiency Anemia And Nonabsolute Iron Deficiency Anemia In End State Renal Disease Undergoing Maintenance Hemodialysis
  9. Immature Platelet Fraction In Dengue Fever And Dengue Hemorrhagic Fever
  10. Cryptococcal Antigen Of Acquired Immune Deficiency Syndrome With Lateral Flow Assay And Cryptococcus Antigen Latex Agglutination System
  11. T-cd4+ And Lipid Profile In Hiv)
  12. Liver Function Parameters Based On Degree Of Liver Fibrosis In Chronic Liver Disease
  13. Brain Derived Neurotrophic Factor (bdnf) As A Prognostic Factor In Severe Head Injury
  14. Hpv Genotype And Hpv Infection Pattern Related To The Histopathological Type Of Cervical Cancer)
  15. Glut 4 In Adipose Tissue
  16. The Diagnostic Value Of Anti Dengue Iga And Anti Dengue Igm/igg In Dengue Virus Infection)
  17. Vitamin D Deficiency And Diseases
  18. Lineage Switch From Acute Lymphoblastic Leukemia To Acute Myelomonocytic Leukemia At A 26 Years Old Woman
  19. The Role For Clinical Pathologist In Hospital Accreditation

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