UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 16 / No. 2 / Published : 2010-03

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Original Article :

Molecul detection of rpob gene mutation in mycobacterium tuberculosis with polymerase chain reaction and singgle strand conformation polymorphism

Author :

  1. P B Notopuro*1
  2. J. Nugraha*2
  3. H Notopuro*3
  1. Alamat Korespondensi : Bagian Patologi Klinik Fakultas Kedokteran Universitas Airlangga, Lantai 4 Gedung Pusat Diagnostik Terpadu, JL. Mayjen. Prof. Dr. Moestopo 6-8 Surabaya
  2. Departemen Patologi Klinik FK Universitas Airlangga – RSUD Dr. Soetomo Jl. Prof. Mayjen Moestopo 6-8 Surabaya
  3. Departemen Biokimia FK Unair, Jl. Prof. Dr. Moestopo 47 Surabaya

Abstract :

Tuberculosis is a chronic infectious disease which is found in the developing as well as the developed country. This disease is one of the community health problems which become the priority programs in the national as well as international health. In the last two decades, they can be found in the emergency tuberculosis problems that is related with the Multi Drug Resistance (MDR) Strain. The detection of rifampicin resistance in M. tuberculosis infection can help clinical laboratory to find the MDR strain. Related to this problem the proportional culture method is still the gold standard for rifampicin resistance detection for M. tuberculosis infection. But this method needs 4−6 weeks to obtain the result, while its sensitivity is not very high. The development of the molecular detection for M. tuberculosis rifampicin resistance in a direct clinical specimen such as sputum, cerebrospinalfLuid, etc. will give an improvement in the diagnosis, because it has an accurate, fast, sensitive and a specific result. Isolates from twenty six of M. tuberculosis derived from the sputum of tuberculosis patients that have failed the tuberculosis treatment, were examined with the proportional culture method. In this study PCR-SSCP were used for the molecular detection of rifampicin resistancy using direct sputum samples. The proportional culture method was used as a gold standard for the rifampicin resistance detection. A set of primers was directed to conserve the region of rpoB gene of M. tubercuLosis. This RNA polymerase gene was encondes?, which is bound on rifampicin. A 157-bp fragment was amplified by PCR and analyzed by SSCP technique. The sensitivity of PCR-SSCP is 80% (high), its specificity is 95.2% (very high), the positive predictive value is 80% and the negative predictive value is 95.2%. Statistically there were no significant difference between the result of PCR-SSCP and the proportional culture method. Based on the study result, the molecular detection technique for rifampicin resistance on M. tuberculosis infection can be used as the screening device /means for Multi Drug Resistance Tuberculosis (MDR-TB), while the clinician waits the culure result.

Keyword :

MDR-TB, PCR-SSCP, culture,


References :

Cooksey RC, Morlock GP, Glickman S, Crawford JT ,(1997) Evaluation of line probe assay kit for characterisation of rpo B mutations in ri New York : J. Clin Microbiol





Archive Article

Cover Media Content

Volume : 16 / No. : 2 / Pub. : 2010-03
  1. Serum Albumin Level In Ischemic And Hemorrhagic Stroke Patients
  2. Microbial Resistance And Sensitivity Pattern To Antimicrobial Drug
  3. Various Bacterial Cultures Related To Their Susceptibility Against Several Types Of Antibiotics
  4. Analysis Of Eosininophil On Acute Interstitial Nephritis
  5. Von Willebrand Kinetic Factor In Adult Dengue Haemorrhagic Fever Patients
  6. Immature To Total Neutrophil (i/t) Ratio As Septic Neonatorum Diagnostic
  7. Hdl Cholesterol Concentration Measured Using Cholestest N Hdl And Hdl-c Plus 3rd Generation Reagents
  8. Molecul Detection Of Rpob Gene Mutation In Mycobacterium Tuberculosis With Polymerase Chain Reaction And Singgle Strand Conformation Polymorphism
  9. The Diagnosis Molecular And Aplication In Treatment Of B & C Hepatitis
  10. Swine Influenza A/h1n1 Confirmed By Pcr
  11. Sd Dengue Duo (ns1, Igg, Igm) Rapid Test For The Diagnosis Of Dengue Virus Infection
  12. The Management Of An Emergency Laboratory

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