UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 15 / No. 1 / Published : 2008-11

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Original Article :

Molecular detection of mycobacterium tuberculosis in sputum with polymerase chain reaction

Author :

  1. P.B. Notopuro*1
  2. J. Nugraha*2
  3. H. Notopuro*3
  1. Departemen Patologi FK-UNAIR - RSU. Dr. Soetomo, Jl. Prof. Dr. Moestopo 6 - 8 Surabaya
  2. Departemen Patologi FK-UNAIR - RSU. Dr. Soetomo, Jl. Prof. Dr. Moestopo 6–8 Surabaya
  3. Departemen Biokimia FK-UNAIR, Jl. Prof. Dr. Moestopo 47 Surabaya

Abstract :

Tuberculosis is a chronic infectious disease which is found in developing and developed country. It is one of community health problems which become priority in national and international health programs. Microbiologic examination is used to establish the diagnosis of tuberculosis beside clinical examination and radiologic examination. Conventional microscopic and culture examination have many limitation ie: such as for example low sensitivity, specificity and need a lot of time. New Molecular technique gives more value in sensitivity, specificity and the time for examination. The aim of this study was to know the diagnostic value of Polymerase Chain Reaction for detection of Mycobacterium tuberculosis in sputum. The sputum was collected from twenty eight patients suspected tuberculosis based on the clinical and radiological examination. The study was performed from September 2006 until July 2007. We did the conventional culture technique as a diagnostic gold standard and molecular technique to detect the Mycobacterium tuberculosis in the sputum. For molecular technique, we used Polymerase Chain Reaction (PCR) with a set of IS6110 region primer which is specific for the Mycobacterium tuberculosis Complex. The sensitivity of PCR with IS6110 region primer is 100% (very high), specificity is 82.4% (high), positive predictive value is 89.7% and negative predictive value is 100%. There was statistically no significant difference between the result of PCR and conventional culture method. Based on the result, the Polymerase Chain Reaction examination with primer IS6110 region primer can be used as the screening tool for tuberculosis infection, while the clinician waits for culture result.

Keyword :

PCR, culture, IS6110 region primer,


References :

Baron EE et all,(1994) Diagnostic Microbiology Philadhelpia : Mosby year book Inc





Archive Article

Cover Media Content

Volume : 15 / No. : 1 / Pub. : 2008-11
  1. (anemia And Iron Deficiency Among Female Adolescents From Junior High School (sltp) Negeri I Curug, Tangerang)
  2. Sgot, Sgpt Activities In Medium And Severe Burn Injuries Patients
  3. Molecular Detection Of Mycobacterium Tuberculosis In Sputum With Polymerase Chain Reaction
  4. The Effect Of Cryopreservation To Epidermal Growth Factor (egf) Level In Amnion Membrane
  5. The Problem Of Vancomycin-resistant Enterococci
  6. Diagnosis Of Filariasis Based On Thick Smear

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