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Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 18 / No. 3 / Published : 2012-01

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Original Article :

Oral immunization with 38-kda adhesin protein of mycobacterium tuberculosis on cd8+ t cells in lung

Author :

  1. Maimun Z Arthamin*1
  2. Agus A Gani*2
  3. Nurani Issiyah*3
  4. Sanarto Santoso*4
  1. Laboratorium Patologi Klinik Fakultas Kedokteran Universitas Brawijaya/RSU dr. Saiful Anwar Malang Jl. JA Suprapto no. 2 Malang
  2. Laboratorium Patologi Klinik Fakultas Kedokteran Universitas Brawijaya/RSU dr. Saiful Anwar Malang Jl. JA Suprapto no. 2 Malang
  3. Program Studi Pendidikan Dokter Fakultas Kedokteran Universitas Brawijaya
  4. Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Brawijaya-RSU dr. Saiful Anwar Malang

Abstract :

  The efficacy of Bacillus Calmette-Guerin (BCG), vaccine against tuberculosis (TB), varies widely, from 0 to 90%; and BCG mainly activates CD4+ T cells, but it fails to activate CD8+ T cells. From the previous study, 38-kDa protein is an adhesin protein. CD8+ T cells play the role in controlling Mycobacterium tuberculosis (M tb) infection and contribute to the memory immunity. The objective of this study was to determine effect of oral immunization by 38-kDa adhesin protein of M tb to increase the level of CD8+ T cells in the lung of BALB/c mice. This study used an experimental with post test control group design. The mice were divided into six groups (each group consist of 4 samples), where Group 1: were immunization orally with 100 μg 38-kDa adhesin protein of M tb and 12 μg ISCOMs. Followed by group 2: 100 μg 38-kDa adhesin protein of M tb, group 3: 50 μg 38-kDa adhesin protein of M tb and 12 μg ISCOMs, and group 4: 50 μg 38-kDa adhesin protein of M tb. Group 5: 12 μg ISCOMs. Group 6: Control. In this study was found increased level of CD8+ T cells in the lung of BALB/c mice after orally immunization with 38-kDa adhesin protein of M tb. The highest level of CD8+ T cells was on group 1, p = 0.000. Also there were found significant differences among the immunized groups, except group 2 and 3, as well as group 5 and 6 also. It can be concluded in this study that oral immunization with 38-kDa adhesin protein of M. tuberculosis could increase the level of CD8+ T cells in the lung of BALB/c mice.     Tingkat ketepatgunaan vaksin Bacillus Calmette-Guerin (BCG) untuk pencegahan terhadap tuberkulosis (TB) berkisar antara 0-90% persen, selain itu BCG hanya dapat mengaktifkan sel T CD4+ tetapi gagal untuk sel T CD8+. Didasari telitian sebelumnya, protein 38-kDa M. tuberculosis (M tb) terbukti merupakan protein adhesin. Sel T CD8+ berperan dalam mengendalikan imunitas dan memori respons imun terhadap infeksi M tb. Tujuan penelitian ini adalah untuk menentukan pemberian protein adhesin 38-kDa M tb lewat rongga mulut yang diduga dapat meningkatkan jumlah sel T CD8+ di dalam paru mencit BALB/c. Penelitian ini merupakan kajian cobaan murni dengan rancangan kelompok pembanding pascauji. Mencit dibagi menjadi 6 kelompok (tiap kelompok terdiri dari 4 ekor). Kelompok 1: diimunisasi dengan protein adhesin 38kDa M tb 100 μg dan ISCOMs 12 μg, selanjutnya kelompok 2: dengan protein adhesin 38kDa 100 μg, kelompok 3: dengan protein adhesin 38 kDa 50 μg dan ISCOMs 12 μg, kelompok 4: dengan protein adhesin 38 kDa 50 μg, kelompok 5: dengan ISCOMs 12 μg,dan kelompok 6: tidak diimunisasi. Hasil telitian menunjukkan bahwa terdapat peningkatan jumlah sel T CD8+ di paru mencit BALB/c setelah pemberian protein adhesin 38-kDa M tb lewat rongga mulut. Peningkatan tertinggi terlihat di kelompok 1, p = 0,000. Dalam kajian ini terdapat perbedaan yang bermakna antar kelompok perlakuan, kecuali antara kelompok 2 dan 3, serta 5 dan 6. Maka dapat disimpulkan bahwa pemberian protein adhesin 38-kDa M tb lewat rongga mulut dapat meningkatkan jumlah sel T CD8+ di paru mencit BALB/c. Temuan ini masih perlu dikaji lebih lanjut untuk potensinya sebagai bahan kandidat vaksin untuk perlindungan terhadap M tb.

Keyword :

38-kDa adhesin protein, mycobacterium tuberculosis, CD8+ T cells, lung, oral immunization,


References :

  1. Senol G, Erer OF, Yalcin YA, Coskun M,Gu ndu AT, Bic men C, Ertas M, Ozkan SAO, (2007). Humoral immune Response Against 38-kDa and 16-kDa Mycobacterial Antigens in Tuberculosis. European : European Respiratory Journal
  2. Utama A, (2007). Tuberkulosis. Jakarta : www.infeksi.com/articles.php?lng=in&pg=57
  3. Martin, C, (2005). The Dream of a Vaccine Against Tuberculosis; New Vaccine Improving or Replacing BCG?. European : European Respiratory Journal
  4. da Fonseca DPAJ, Joosten D, van der Jee R, jue DL, Singh M, Vordermeier HM, Snippe H, Verheul AFM, (1998). Identification of New Cytotoxic T-Cell Epitopes on the 38-Kilodalton Lipoglycoprotein of Mycobacterium tuberculosis by Using Lipopeptides. USA : Infection and Immunity
  5. Sarhan, MAA, (2007). Progress in Tuberculosis Vaccines Development. USA : Research Journal of Medicine and Medical Sciences


   


Archive Article

Cover Media Content

Volume : 18 / No. : 3 / Pub. : 2012-01
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  2. Association hla-drb1* and hla-dqb1* with serum igm-rf with rheumatoid arthritis
  3. Platelets of dengue hemorrhagic fever
  4. The diagnostic value of tb antigen using rapid test device (tb ag) for pulmonary tuberculosis
  5. Antimicrobial susceptibility test of pathogenic aerobic bacteria at the internal medicine ward
  6. Correlation of liver functions test, and the grade of dengue hemorrhagic fever in children
  7. Pulmonary cryptosporidiosis in tbc patients
  8. Mycobacterium tuberculosis and pcr
  9. Oral immunization with 38-kda adhesin protein of mycobacterium tuberculosis on cd8+ t cells in lung
  10. Thrombocytes count in acute coronary syndrome related to low molecular weight heparin (lmwh)}
  11. Acute fatty liver of pregnancy
  12. Cold agglutinins in a community acquired pneumonia patient