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Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 18 / No. 1 / Published : 2011-01

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Original Article :

Phyllanthus niruri l the effects of extract on cellular immunity mice

Author :

  1. Ima Arum L*1
  2. Purwanto AP*2
  3. Henna Rya*3
  1. Departemen Patologi Klinik, Fakultas Kedokteran Universitas Mataram Nusa Tenggara Barat
  2. Bagian Patologi Klinik Fakultas Kedokteran Universitas Diponegoro Semarang/RSUP Dr. Kariadi Semarang
  3. Bagian Patologi Klinik Fakultas Kedokteran Universitas Diponegoro Semarang/RSUP Dr. Kariadi Semarang

Abstract :

Typhoid fever is a problem in the developing country. Phyllanthus niruri L (meniran) is a traditional plant that can be used as an immunomodulator on cellular or humeral immunity. By giving Phyllanthus niruri L extract to patient who suffers typhoid fever can increase cellular immunity responses in intracellular bacteria infection (S.typhimurium).This research was an experimental study using the post test-only control group design to proof of the effects of Phyllanthus niruri L on cellular immunity balb/c infected by salmonella typhimurium.  The samples were taken from thirty male Balb/c mice which were divided into five groups including P1 (infected by S.typhimurium and PnL extract 125 μg), P2 (infected by S.typhimurium and PnL extract 250 μg), P3 (infected by S.typhimurium and PnL extract 500 μg), K1 (infected by S.typhimurium) and K2 (healthy mice). In this study was applied parametric test of one way ANOVA and post hoc test Bonfferoni. It was known that phyllanthus niruri L increased the number of activated lymphocytes and phagocyte ability of macrophage (p=0,003 and p=0,001, respectively). By Bonfferoni post hoc test resulted that no significantly difference between the treated groups (increased of dose). There were no significant difference between the number of leukocyte, the difference count of lymphocyte and the nitrite oxide produced among the treatment groups and controls. Phyllanthus niruri L dose 250 μg increased the activated lymphocyte and index phagocytes. To find out the effects of Phyllanthus niruri L compare to antibiotic and Phyllanthus niruri L combination, continuing/advancing studies are needed.     Demam tifoid merupakan penyakit yang masih menjadi masalah di negara berkembang. Phyllanthus niruri L (meniran) merupakan tanaman tradisional yang mempunyai manfaat sebagai imunomodulator pada penyakit yang membutuhkan pertahanan sistem imun seluler maupun humoral. Pemberian ekstrak meniran dapat meningkatkan respons imun seluler pada infeksi bakteri intraseluler (S.typhimurium). Tujuan penelitian ini untuk membuktikan adanya pengaruh pemberian Phyllanthus niruri L dosis 125, 250 dan 500 μg selama 7 hari terhadap respons imunitas seluler pada mencit Balb/C yang diinfeksi dengan Salmonella typhimurium. Penelitian eksperimental laboratorik menggunakan rancangan the post test-only control group.  Mencit Balb/C jantan 30 ekor dibagi menjadi 5 kelompok yaitu P1 (diinfeksi S.typhimurium dan ekstrak PnL 125 μg), P2 (diinfeksi S.typhimurium dan ekstrak PnL 250 μg), P3 (diinfeksi S.typhimurium dan ekstrak PnL 500 μg), K1 (diinfeksi S.typhimurium) dan K2 (sehat). Uji beda menggunakan uji parametrik one way ANOVA dilanjutkan post hoc test LSD.   Phyllanthus niruri L meningkatkan jumlah limfosit tergiatkan dan kemampuan fagositosis makrofag secara bermakna (p=0,003 dan p=0,001), dengan uji post hoc Bonfferoni tidak ada perbedaan bermakna antar kelompok perlakuan (perbedaan dosis). Tidak ada perbedaan yang bermakna pada jumlah leukosit, hitung jenis limfosit dan produksi NO pada kelompok perlakuan dan kelompok kontrol. Pemberian Phyllanthus niruri L dapat meningkatkan jumlah limfosit yang tergiatkan dan indeks fagositosis namun tidak sejalan dengan peningkatan dosis. Penelitian lanjut tentang pengaruh Phyllanthus niruri L dibandingkan antibiotika kombinasi dengan Phyllanthus niruri L.

Keyword :

Phyllanthus niruri L, leukocyte, percentage of lymphocyte, , activated lymphocyte, nitrit oxide and phagocyte abillity of macrophage,


References :

  1. WHO, (2005). Drug resistant salmonella. Geneva : WHO
  2. 2. Alwi I, Setiati S, Setiyohadi B, Simadibrata M, Sudoyo AW, (2006). Buku Ajar Penyakit Dalam. Jakarta : Pusat Penerbitan Departemen Ilmu Penyakit Dalam FK UI
  3. Katzung BG, (1997). Farmakologi dasar dan klinik. Jakarta : EGC
  4. Munasir Z, (2002). Manfaat pemberian ekstrak phyllanthus niruri sebagai imunostimulator pada penyakit infeksi anak. Jakarta : http://www.tnial.mil.id/cakrawala. php3
  5. Kresno SB, (2001). Imunologi: Diagnosis dan prosedur laboratorium. Jakarta : Balai Penerbit FK.UI


   


Archive Article

Cover Media Content

Volume : 18 / No. : 1 / Pub. : 2011-01
  1. Aerob microbes pattern and antimicrobial sensitivity of diabetic foot ulcer
  2. Plasma levels of interleukin 10 (il-10) in malaria and anaemia
  3. Identification of cryptosporidiosis in paediatric hiv-infected patients with chronic diarrhoea at paediatric gastro
  4. Immunoglobulin a in dengue hemorrhagic fever
  5. (comparison of determination for thyroxine with enzyme linked immunofluorescent assay (elfa) and enzyme linked immunosorbant assay (elisa)}
  6. Plasma interleukin-10 and cd4+ lymphocyte-t in hiv infected patients
  7. Detecting fluoroquinolone resistance of salmonella sp using nalidixic acid succeptibility test
  8. Phyllanthus niruri l the effects of extract on cellular immunity mice
  9. Fitoestrogen dalam beberapa daun dan buah
  10. Diagnostic test nt pro natriuretic peptide (ntprobnp) on congestive heart failure
  11. Human immunodeficiency virus (hiv) infection in babies and children
  12. Decompensated cirrhosis hepatic in children
  13. Human resources management in the clinical laboratory