UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 18 / No. 1 / Published : 2011-01

Related with : Scholar   Yahoo!   Bing

Original Article :

Plasma interleukin-10 and cd4+ lymphocyte-t in hiv infected patients

Author :

  1. Kadek Mulyantari*1
  2. Endang Retnowati*2
  3. Nasronudin*3
  1. Dep. Patologi Klinik, FK. Universitas Udayana/RS. Sanglah
  2. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya
  3. Departemen Ilmu Penyakit Dalam FK Unair/ RSUD Dr. Soetomo Surabaya

Abstract :

  HIV & AIDS have become a global problem throughout the world including Indonesia. The incidence is rapidly increasing. Various treatment and efforts have been carried out but until now have not yet been succeeded, the mortality remains high. Examination of CD4+ lymphocyte-T which is carried out to determine the immune status and monitoring the treatment has some limitation. Besides the degradation of CD4+ lymphocyte-T, the increase of the disease progression is also followed by an increase of interleukin-10 as well. The determination of interleukin-10 is expected to be use as an alternative examination if CD4+ lymphocyte-T can not be performed. To analyze the correlation between the levels of plasma interleukin-10 and amount of CD4+ lymphocyte-T in the asymptomatic HIV infected patients. A cross sectional, observational analytical study of 41 patients with HIV infection stage I, has been conducted from March ― April 2009, at the Intermediate Infectious Disease Care Unit Dr. Soetomo General Hospital, Surabaya. The diagnosis of HIV patients was based on positive result of HIV test using three (3) different methods. Eight mL venous blood were taken from  each patient, 6 mL is put into a heparin tube for the examination of  IL-10 plasma with ELISA method and 2 mL is put into a K3EDTA tube for examining the CD4+ lymphocyte-T using flowcytometry.  The results showed of plasma IL-10 level and the amount of CD4+ lymphocyte-T, which were analyzed by Pearson correlation test to determine the correlation between the two (2) variabels. The IL-10 levels in HIV patients were 3.80- 44.50 pg/mL (mean 18.09 pg/mL, SD 8.84 pg/mL). The amount of absolute CD4+ lymphocyte-T was 5 - 846 cells/µL (mean 322.07 cells/µL and SD 221.89 cells/µL), while the amount of percentage CD4+ lymphocyte-T was 0.41 – 29.48 % (mean 13.99 %, SD 7.62%). Statistical analyzes show a significant negative correlation either between plasma IL-10 level with absolute CD4+lymphocyte-T and plasma IL-10 level with percentage of CD4+ lymphocyte-T, each level of r was  – 0. 652 and – 0. 683. A significant negative correlation was also formed between plasma IL-10 level and the amount of CD4+ lymphocyte-T in the HIV infected patients. In the HIV infected patients, the increase of plasma IL-10 level was followed by a decrease of CD4+ lymphocyte-T.     HIV & AIDS merupakan masalah mendunia dengan angka kejadian yang terus meningkat. Berbagai jenis pengobatan dan upaya yang sudah dilakukan dianggap belum berhasil sepenuhnya, sehingga angka kematian tetap tinggi. Pemeriksaan jumlah limfosit-T CD4+ untuk menentukan status imun dan pemantauan pengobatan memiliki beberapa keterbatasan, selain terjadi penurunan jumlah limfosit-T CD4+, pada peningkatan keparahan penyakit juga diikuti peningkatan kadar interleukin-10. Pemeriksaan interleukin-10 diharapkan dapat sebagai pengganti pemeriksaan jika jumlah limfosit-T CD4+ tidak dapat ditentukan. Kajian ini dilakukan untuk mengetahui kenasaban antara kadar interleukin-10 plasma dan jumlah limfosit-T CD4+ di penderita terinfeksi HIV. Penelitian bersifat amatan potong silang (cross sectional observasional). Sampel terdiri dari 41 penderita terinfeksi HIV tahap I, yang dirawat di Unit Perawatan Intermediit Penyakit Infeksi RSUD Dr. Soetomo Surabaya mulai Maret sampai dengan April 2009. Diagnosis HIV berdasarkan hasil uji HIV positif dengan menggunakan tiga cara yang berbeda. Pengambilan darah vena dilakukan sebanyak delapan mL, yang enam mL dimasukkan ke dalam tabung heparin untuk pemeriksaan kadar interleukin-10 plasma dengan metode ELISA. Kemudian yang 2 mL darah dimasukkan ke dalam tabung K3EDTA untuk pemeriksaan jumlah limfosit-T CD4+ menggunakan metode sitometri aliran (flowcytometry). Hasil periksaan kadar interleukin-10 plasma dan jumlah limfosit-T CD4+ dianalisis dengan uji kenasaban Pearson untuk menentukan kenasaban antara kedua variabel tersebut. Kadar IL-10 plasma penderita terinfeksi HIV berkisar antara 3,80 pg/mL hingga 44,50 pg/mL (rerata 18,09 pg/mL, SD 8,84 pg/mL). Hasil periksaan jumlah limfosit-T CD4+ mutlak penderita terinfeksi HIV berkisar antara 5 sel/µL hingga 846 sel/µL (rerata 322,07 sel/µL dan SD 221,89 sel/µL).  Berdasarkan nilai persentase, berkisar antara 0,41 hingga 29,48 % (rerata 13,99 %, SD 7,62%). Analisis statistik menunjukkan kenasaban negatif yang berarti baik antara kadar IL-10 plasma dengan jumlah limfosit-T CD4+ mutlak maupun antara kadar IL-10 dengan jumlah limfosit-T CD4+ persentase dengan nilai r masing-masing – 0, 652 dan – 0, 683. Kenasaban negatif yang terdapat berarti antara kadar IL-10 plasma dan jumlah limfosit-T CD4+ di penderita terinfeksi   HIV. Di penderita terinfeksi HIV, peningkatan kadar IL-10 plasma akan diikuti penurunan jumlah limfosit-T CD4+. Pemeriksaan IL-10 dapat digunakan sebagai salah satu pemeriksaan alternatif jika pemeriksaan jumlah limfosit T-CD4+ tidak dapat dilakukan. Selain itu pemeriksaan Il-10 plasma juga dapat digunakan sebagai salah satu pertimbangan dalam penatalaksanaan penderita terinfeksi HIV serta membantu dalam pemantauan pemberian pengobatan anti retrovirus pada penderita HIV & AIDS.

Keyword :

Interleukin-10, CD4+ lymphocyte-T, human immunodeficiency virus,


References :

Depkes RI,(2007) Pedoman Nasional Terapi Antiretroviral Jakarta : Depkes RI

Depkes RI,(2006) Pedoman Pelayanan Konseling dan Testing HIV/AIDS Secara Sukarela (Voluntary Counselling and Testing) Jakarta : Depkes RI

Nasronudin,(2007) Pengaruh HIV terhadap Perubahan Kadar ROS dan Hsp. HIV & AIDS Pendekatan Biologi Molekuler, Klinis, dan Sosial Surabaya : Airlangga University Press

Gallant JE, Hoffmann C,(2008) CD4 Cell Count. HIV Guide USA : CD4 Cell Count. HIV Guide

Beverley P, Helbert M,(2001) Immunology of AIDS London : BMJ Publishing Group





Archive Article

Cover Media Content

Volume : 18 / No. : 1 / Pub. : 2011-01
  1. Aerob Microbes Pattern And Antimicrobial Sensitivity Of Diabetic Foot Ulcer
  2. Plasma Levels Of Interleukin 10 (il-10) In Malaria And Anaemia
  3. Identification Of Cryptosporidiosis In Paediatric Hiv-infected Patients With Chronic Diarrhoea At Paediatric Gastro
  4. Immunoglobulin A In Dengue Hemorrhagic Fever
  5. (comparison Of Determination For Thyroxine With Enzyme Linked Immunofluorescent Assay (elfa) And Enzyme Linked Immunosorbant Assay (elisa)}
  6. Plasma Interleukin-10 And Cd4+ Lymphocyte-t In Hiv Infected Patients
  7. Detecting Fluoroquinolone Resistance Of Salmonella Sp Using Nalidixic Acid Succeptibility Test
  8. Phyllanthus Niruri L The Effects Of Extract On Cellular Immunity Mice
  9. Fitoestrogen Dalam Beberapa Daun Dan Buah
  10. Diagnostic Test Nt Pro Natriuretic Peptide (ntprobnp) On Congestive Heart Failure
  11. Human Immunodeficiency Virus (hiv) Infection In Babies And Children
  12. Decompensated Cirrhosis Hepatic In Children
  13. Human Resources Management In The Clinical Laboratory

Information CPML