UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 18 / No. 1 / Published : 2011-01

Related with : Scholar   Yahoo!   Bing

Original Article :

Plasma levels of interleukin 10 (il-10) in malaria and anaemia

Author :

  1. I Nyoman Wande*1
  2. Endang Retnowati*2
  3. Juli Soemarsono*3
  1. Dep. Patologi Klinik, FK. Universitas Udayana/RS. Sanglah
  2. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya
  3. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya

Abstract :

  Anaemia is an important complication of malaria, and its pathogenesis is not well understood. High level of the Th2 cytokine (such as IL-10), which counteract the Th1 cytokine, might prevent the development of severe malarial anaemia. The purpose of this study was to know the comparation between the plasma level of IL-10 in malaria falciparum patients with anaemia and without anaemia. The plasma level of IL-10 was examined in 16 malaria falciparum patients with anaemia and 16 malaria caused by P. falciparum patients without anaemia samples taken from patients at the primary health centres in West Lombok and Centre Lombok during March until July 2008. The samples were measured using ELISA. The concentration of haemoglobin (Hb) was measured using hematological analyzer. The anaemia concentration of Hb is < 11 g/dL. The results were analyzed using two (2) sample t test with SPSS ver.13.The plasma level of IL-10 in malaria patients caused by P. falciparum with anaemia was 8.81(3.04) [mean(SD)] pg/mL where as the plasma level of IL-10 in malaria falciparum patients without anaemia was 47.99(25.26) pg/mL. The mean of IL-10 level in malaria falciparum patients with anaemia was significantly lower than that of malaria patients caused by P. falciparum without anaemia (p=0.000).     Anemia merupakan salah satu komplikasi yang penting di malaria, dan patogenesisnya di penyakit tersebut belum sepenuhnya difahami. Kadar sitokin Th2 (seperti IL-10) yang tinggi, dan menetralkan sitokin Th1, mungkin dapat mencegah perkembangan anemia di pasien malaria yang lebih berat. Tujuan penelitian ini adalah untuk mengetahui kadar IL-10 plasma dengan membandingkan yang terdapat di antara penderita malaria dengan anemia dan tanpa anemia. Kadar IL-10 plasma di 16 pasien malaria penyebab P. falciparum yang beranemia dan 16 pasien malaria penyebab P. falciparum tanpa anemia yang berasal dari Puskesmas Lombok Barat dan Lombok Tengah selama bulan Maret sampai Juli 2008, diukur dengan menggunakan cara ELISA. Kadar hemoglobin (Hb) diukur dengan menggunakan alat hematology analyzer. Anemia adalah kepekatan Hb <11 g /dL. Hasil telitian dianalisis menggunakan uji statistik t dua sampel bebas dengan SPSS ver.13. Kadar rerata IL-10 plasma di pasien malaria penyebab P. falciparum beranemia adalah 8,81 ± 3,04 pg/mL sedangkan kadar rerata IL-10 plasma di pasien malaria penyebab P. falciparum tanpa anemia adalah 47,99 ± 25,26 pg / mL. Rerata kadar IL-10 plasma di pasien malaria beranemia secara berarti lebih rendah daripada rerata kadar IL-10 plasma pada penderita malaria tanpa anemia (p = 0,000).

Keyword :

Malaria falciparum, anaemia, IL-10,


References :

Depkes,(2006) Pedoman Penatalaksanaan Kasus Malaria di Indonesia Jakarta : Direktorat Jenderal Pengendalian Penyakit dan Penyehatan Lingkungan

Riley EM,(1999) Is The T-cell Priming Required For Initial Pathology in Malaria Infections? USA : Immunology Today

McGuire W, Knight JC, Hill AV, Allsopp CE, Greenwood BM, Kwiatkowski D,(1999) Severe malarial anemia and cerebral malaria are associated with different tumor necrosis factor promoter alleles USA : J.Infect.Dis

Menendez C, Fleming AF, alonso PL,(2000) Malaria-related Anaemia. Parasitology Today USA : Elsevier Science Ltd

Fernandes AAM, Carvalho LJM, Zanini GM, Ventura AMRS, Souza JM, Cotias PM, Silva-Filho IL, and Danie,(2008) Similar cytokine responses and degrees of anemia in patients with Plasmodium falciparum and Plasmodium vivax infections in the Brazilian Amazon region. Clinical and Vaccine Immunology USA : National Center for Biotechnology Information, U.S. National Library of Medicine





Archive Article

Cover Media Content

Volume : 18 / No. : 1 / Pub. : 2011-01
  1. Aerob Microbes Pattern And Antimicrobial Sensitivity Of Diabetic Foot Ulcer
  2. Plasma Levels Of Interleukin 10 (il-10) In Malaria And Anaemia
  3. Identification Of Cryptosporidiosis In Paediatric Hiv-infected Patients With Chronic Diarrhoea At Paediatric Gastro
  4. Immunoglobulin A In Dengue Hemorrhagic Fever
  5. (comparison Of Determination For Thyroxine With Enzyme Linked Immunofluorescent Assay (elfa) And Enzyme Linked Immunosorbant Assay (elisa)}
  6. Plasma Interleukin-10 And Cd4+ Lymphocyte-t In Hiv Infected Patients
  7. Detecting Fluoroquinolone Resistance Of Salmonella Sp Using Nalidixic Acid Succeptibility Test
  8. Phyllanthus Niruri L The Effects Of Extract On Cellular Immunity Mice
  9. Fitoestrogen Dalam Beberapa Daun Dan Buah
  10. Diagnostic Test Nt Pro Natriuretic Peptide (ntprobnp) On Congestive Heart Failure
  11. Human Immunodeficiency Virus (hiv) Infection In Babies And Children
  12. Decompensated Cirrhosis Hepatic In Children
  13. Human Resources Management In The Clinical Laboratory

Information CPML