UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 20 / No. 1 / Published : 2013-01

Order : 6, and page :25 - 28

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Original Article :

Protein disulfide isomerase family a member 4 (pdia4) in metastatic breast cancer

Author :

  1. Stefanus Lembar*1
  2. Sheela R Bororing*2
  3. Lilis*3
  1. Dosen Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran

Abstract :

Protein disulfide isomerase (PDI) is a member of  thioredoxin superfamily that is secreted in the lumen of the endoplasmic reticulum (ER) in mammalian cells. PDI catalyzes disulfide bond formation, reduction, or isomerization of newly synthesized proteins in the lumen of the RE. PDIA4 is a member of the PDI family, which is also expressed in the mammary gland. Low expression of PDIA4 probably as a result of the stress of RE in hypoxic conditions. These conditions will enhance the ability of tumor cells in mammary tissue to divide aggressively and metastasize. A total of 61 breast cancer patients aged 23−90 years (60 women and 1 man) in Jakarta, Indonesia have participated in this study after signing the informed consent letter. The diagnosis of metastasis is confirmed by the examination of bone scanning. Measurement of PDIA4 concentration is performed by the quantitative enzyme-linked immunosorbent assay (ELISA) using a commercial kits. Based on the examination of the bone scanning, there were 23 (62.5%) subjects with metastatic and 38 (37.5%) subjects non-metastatic. The mean of age of the subjects with metastatic were significantly higher than non-metastatic subjects (p=0.028), which is 51.4 (45.9-56.8) and 44.2 (40.4-48.0). The median of PDIA4 concentrations in serum of subjects with metastatic, i.e. 7.7 (1.0-37.2) ng/ml was significantly lower than non-metastatic subjects (p=0.044), i.e. 9.4 (2.2 – 69. 6) ng/mL. The concentration of PDIA4 in serum of breast cancer patients with metastatic were significantly lower than non-metastatic patients. It shows that the concentration of PDIA4 have decreased in incidence of metastatic breast cancer.     Protein disulfida isomerase (PDI) adalah anggota superfamili thioredoxin yang sangat banyak disekresikan lumen retikulum endoplasma (RE) dalam sel mamalia. PDI mengkatalisis pembentukan ikatan disulfida, mengurangi, atau isomerisasi protein yang baru dibuat di dalam lumen RE. PDIA4 merupakan anggota famili PDI yang salah satunya diekspresikan di kelenjar payudara. Ekspresi yang rendah PDIA4 kemungkinan terjadi sebagai akibat stres RE dalam kondisi hipoksia. Kondisi lingkungan mikro yang kekurangan gizi dan oksigen akan meningkatkan kemampuan sel tumor di jaringan payudara untuk membelah secara agresif dan bermetastasis. Sebanyak 61 penderita karsinoma mammae yang berusia antara 23−90 tahun (60 perempuan dan satu/1 laki-laki) di Jakarta telah berperan-serta dalam penelitian ini setelah menandatangani surat persetujuan tindakan. Diagnosis metastasis ditetapkan berdasarkan pemeriksaan bone scaning. Pengukuran kepekatan PDIA4 dengan metode kuantitatif enzyme-linked immunosorbent assay (ELISA) menggunakan perangkat komersial. Berdasarkan pemeriksaan bone scaning, terdapat 23 (62,5%) subjek dengan metastasis dan 38 (37,5%) yang non-metastasis. Rerata usia subjek dengan metastasis secara bermakna lebih tinggi daripada yang non-metastasis (p = 0,028), yaitu 51,4 (95%CI: 45,9-56,8) dan 44,2 (95%CI: 40,4-48,0). Median kepekatan PDIA4 dalam serum di subjek dengan metastasis, yaitu 7,7 (1,0-37,2) ng/mL secara bermakna lebih rendah dibandingkan dengan yang non-metastasis, yaitu 9,4 (2,2--69,6) ng/mL dengan nilai p = 0,044. Kepekatan PDIA4 dalam serum di subjek dengan metastasis secara bermakna lebih rendah daripada yang non-metastasis. Hal tersebut menunjukkan bahwa terjadi penurunan kepekatan PDIA4 di  metastasis karsinoma mammae.

Keyword :

Breast cancer, metastatic, PDIA4,


References :

Tjindarbumi D, Mangunkusumo R,(2002) Cancer in Indonesia Jepang : Jpn J Clin Oncol





Archive Article

Cover Media Content

Volume : 20 / No. : 1 / Pub. : 2013-01
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  5. Microrganisms And Sensitivity Tests Of Diabetic Foot
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