UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 14 / No. 3 / Published : 2008-07

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Original Article :

Rapid test for igm antibodies salmonella typhi of typhoid fever

Author :

  1. Rini Riyanti*1
  2. Prihatini*2
  3. Siti Rochmatoen*3
  1. Departemen Patologi Klinik, Fakultas Kedokteran Universitas Airlangga/RSU Dr. Soetomo, Surabaya
  2. Departemen Patologi Klinik, Fakultas Kedokteran Universitas Airlangga/RSU Dr. Soetomo, Surabaya
  3. Departemen Patologi Klinik, Fakultas Kedokteran Universitas Airlangga/RSU Dr. Soetomo, Surabaya

Abstract :

Typhoid fever is caused by Salmonella typhi. The definitive diagnosis can be made by isolation of Salmonella typhi from blood, bone marrow or other body fluids. To support the clinical diagnosis of typhoid fever in Indonesia, where most hospitals and health centres have no facilities for cultures, a rapid test for the detection of lipopolysaccharides (LPS) Salmonella typhi-specific IgM antibodies was evaluated on serum samples from patients with typhoid fever. This study is proposed to know the rapid test diagnostic value for the detection of lipopolysaccharides (LPS) Salmonella typhi-specific IgM antibodies. A cross sectional, observational analytical study on 27 typhoid fever and 25 dengue hemorrhagic fever patients of the Dr. Soetomo Hospital, Dr. M Soewandhi General Hospital and Gotong-Royong Clinic has been conducted from January – May 2007. The diagnosis of typhoid fever patients was based on positive gall culture while the diagnosis of dengue hemorrhagic fever was based on negative gall culture, positive serology examination for dengue hemorrhagic fever and a recovery from dengue hemorrhagic fever with standard treatment. The sera from patients were examined using a rapid test for the detection of lipopolysacharides (LPS) Salmonella typhi specific IgM antibodies from Amgenix Onsight of the first blood samples (collected on admission to the hospital) the rapid test for IgM antibodies showed the following: sensitivity 70.4%, specificity 80.0%, positive predictive value 79.2%, negative predictive value 71.4%, diagnostic efficiency 75% respectively. Of the second blood samples (collected 2–3 weeks during the illness) therapid test for IgM antibodies showed the following: sensitivity 88.9%, positive predictive value 82.8%, negative predictive value 87.0%, and diagnostic efficiency 84.6% respectively. The rapid test for IgM antibodies has a high diagnostic value for typhoid fever. The assay uses stabilized components which can be stored at room temperature; the test does not require special equipment and may be used in health centres that have no facilities for culture.

Keyword :

Typhoid fever, rapid test for IgM antibodies,


References :

Butler T, Scheld WM,(2004) Typhoid Fever Philadhelpia : Saunders





Archive Article

Cover Media Content

Volume : 14 / No. : 3 / Pub. : 2008-07
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  2. Lactic Acid Profile In Controlled And Uncontrolled Diabetes Mellitus Patients
  3. Ldl Related Risk Markers For Coronary Heart Disease
  4. Evaluation Of Sensitivity And Specificity Of Troponin I And Troponin T As Cardiac Biochemical Markers In The Early Diagnosis Of Acute Myocardial Infarction (ami)
  5. Rapid Test For Igm Antibodies Salmonella Typhi Of Typhoid Fever
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  7. The Corelation Between Leukemia Plasma Cell And Myelofibrosis
  8. Evaluation Of Immunochromatography Method For Detection Of Antibody To Tuberculosis Antigen In Lung Tuberculosis Patients
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