UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 16 / No. 2 / Published : 2010-03

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Original Article :

Sd dengue duo (ns1, igg, igm) rapid test for the diagnosis of dengue virus infection

Author :

  1. Diah Puspita Rini*1
  2. Aryati*2
  1. Departemen Patologi Klinik FK Universitas Airlangga – RSUD Dr. Soetomo Jl. Prof. Mayjen Moestopo 6-8 Surabaya
  2. Departemen Patologi Klinik FK Universitas Airlangga – RSUD Dr. Soetomo Jl. Prof. Mayjen Moestopo 6-8 Surabaya

Abstract :

Dengue virus infection can cause dengue hemorrhagic fever (DHF) which is still a major health problem in Indonesia. The diagnosis of DHF is established based on WHO criteria and IgM/IgG antidengue serologic markers. A rapid method is needed for the detecting or screening the disease. Antigen detection (NS1) can be performed by immunochromatography (rapid test) or enzyme -linked immunosorbent assay (ELISA). Recently, a rapid test to detect NS1 as an antigen and IgM/IgG antidengue to differentiate primary and secondary dengue virus infection is available in one cassette. This study evaluated the new commercial dengue rapid test, SD Dengue Duo for the detection of both antigen and antibodies to dengue virus. Serum samples used in this study were collected from 33 dengue virus infection patients according to WHO criteria and admitted in the Tropical Ward, Dr. Soetomo Hospital. Samples were taken twice, during acute and convalescent phase. SD Dengue Duo (NS1, IgG, IgM) rapid test was used and confirmed by ELISA as the gold standard. To determine the diagnostic specificity 20 samples of non dengue virus infection (typhoid fever and malaria) confirmed by laboratory tests were used. In the acute phase, SD Dengue Duo (NS1, IgG, IgM) rapid test showed IgG sensitivity 94.7% (18/19), specificity 92.9% (13/14), IgM sensitivity 85% (17/20), specificity 100% (13/13), NS1 sensitivity 50% (10/20), specificity 100% (13/13). In the convalescent phase, SD Dengue Duo (NS1, IgG, IgM) rapid test showed antidengue IgG sensitivity 96.3% (26/27), specificity 66.7% (4/6), IgM sensitivity 95% (22/23), specificity 80% (8/10), NS1 sensitivity 42.9% (3/7), specificity 100% (26/26). To establish the diagnosis of dengue virus infection, not only NS1, but also antidengue IgM/IgG is needed. SD Dengue Duo containing dengue NS1 antigen combined with IgG/IgM test in one cassette is a rapid, practical and has a high validity diagnostic result.

Keyword :

Dengue virus infection, NS1, antidengue IgM and IgG, rapid test,


References :

Soegijanto S, Sustini F, Wirahjanto A,(2004) Demam berdarah dengue, tinjauan dan temuan baru di era 2003 Surabaya : Airlangga University Press





Archive Article

Cover Media Content

Volume : 16 / No. : 2 / Pub. : 2010-03
  1. Serum Albumin Level In Ischemic And Hemorrhagic Stroke Patients
  2. Microbial Resistance And Sensitivity Pattern To Antimicrobial Drug
  3. Various Bacterial Cultures Related To Their Susceptibility Against Several Types Of Antibiotics
  4. Analysis Of Eosininophil On Acute Interstitial Nephritis
  5. Von Willebrand Kinetic Factor In Adult Dengue Haemorrhagic Fever Patients
  6. Immature To Total Neutrophil (i/t) Ratio As Septic Neonatorum Diagnostic
  7. Hdl Cholesterol Concentration Measured Using Cholestest N Hdl And Hdl-c Plus 3rd Generation Reagents
  8. Molecul Detection Of Rpob Gene Mutation In Mycobacterium Tuberculosis With Polymerase Chain Reaction And Singgle Strand Conformation Polymorphism
  9. The Diagnosis Molecular And Aplication In Treatment Of B & C Hepatitis
  10. Swine Influenza A/h1n1 Confirmed By Pcr
  11. Sd Dengue Duo (ns1, Igg, Igm) Rapid Test For The Diagnosis Of Dengue Virus Infection
  12. The Management Of An Emergency Laboratory

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