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Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 21 / No. 2 / Published : 2015-03

TOC : 8, and page :145 - 152

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Original Article :

The 38 kda adhesin protein of mycobacterium tuberculosis and macrophage of the lung

Author :

  1. Sumarno*1
  2. Maimun Zulhaidah A*2
  3. Rahmawati*3
  4. Bethasiwi Purbasari*4
  1. Laboratorium Mikrobiologi FK Unibraw
  2. Bagian Patologi Klinik FK Unibraw - RSUD dr. Saiful Anwar Malang
  3. Program Studi Pendidikan Dokter FK Unibraw
  4. Program Studi Pendidikan Dokter FK Unibraw

Abstract :

Tuberculosis (TB) in Indonesia is at the third level in the world. The effectiveness of TB vaccine in lung TB. prevention varies, thus, this has motivated the researcher to explore based material of vaccine with a higher effectivity. One of the alternate vaccines that has developed, is the sub unit one made from adhesin protein. The aim of this study was to know the kind of oral administration of 38 kDa adhesin protein of M. tuberculosis in determining so that it can increase the level of macrophage in lungs of BALB/c mice. This study was an experimental work using BALB/c male mice that were immunized with 38 kDa adhesin protein of Mycobacterium tuberculosis orally. The samples were chosen at random and divided into six (6) groups that consisted of: “100 μg protein +adjuvant Immunostimulating Complex (ISCOM)” group (n=5), “50 μg + adjuvant ISCOM” group (n=5), “100 μg” group (n=5), “50 μg” group (n=5), “ISCOM” group (n=5) and “negative control” group (n=5). The measurement of the variable in this study was the number of macrophages . The results showed that the increasing number of macrophage had significant differences between each group. ANOVA test showed a significant level at p<0.05. The conclusion of this study was that 38 kDa adhesin protein of M. tuberculosis peroral could increase the level of macrophage in the lung of BALB/c mice. The highest level of macrophages was the group induced by 100 μg 38 kDa adhesin protein of M. tuberculosis and adjuvant ISCOM. orally Saat ini jumlah kasus tuberkulosis (TB) di Indonesia berada di peringkat ketiga terbanyak di dunia. Ketepatgunaan vaksin BCG yang beragam terhadap TB paru mendorong peneliti untuk dapat mengetahui bahan dasar vaksin dengan ketepatgunaan yang lebih tinggi, yaitu mencarinya lewat penelitian. Salah satu pilihan yang dikembangkan adalah vaksin subunit yang menggunakan protein adhesin. Penelitian ini bertujuan untuk mengetahui tingkat pemberian protein adhesin 38 kDa M. tuberkulosis lewat mulut dengan menentukannya lewat pengkajian. Karena kajian tersebut dapat meningkatkan jumlah sel makrofag di paru mencit BALB/C. Penelitian ini merupakan penelitian percobaan yang menggunakan mencit galur BALB/c jantan yang divaksin dengan protein adhesin 38 kDa lewat mulut. Sampel dipilih dengan cara acak yang dibagi dalam enam (6) kelompok, yaitu: kelompok “protein 100 μg+ Immunostimulating Complex (ISCOM)” (n=5), 50 μg+ Immunostimulating Complex (ISCOM)” (n=5), 100 μg” (n=5), 50 μg” (n=5), kelompok “ISCOM” (n=5) dan “pembanding negatif” (n=5). Variabel yang diukur pada penelitian ini adalah jumlah sel makrofag. Hasil meneliti ini menunjukkan peningkatan jumlah sel makrofag yang berbeda secara bermakna antar kelompok. Dalam uji ANOVA, didapatkan nilai kemaknaan p<0,05. Berdasarkan telitian ini dapat disimpulkan bahwa pemberian imunisasi lewat mulut protein adhesin 38 kDa M. tuberculosis dapat meningkatkan jumlah sel makrofag di dalam paru mencit BALB/c. Peningkatan jumlah sel makrofag yang paling tinggi didapatkan pada pemberian imunisasi lewat mulut protein adhesin 38 kDa M. tuberkulosis 100 μg dengan bahan penambah Immunostimulating Complex (ISCOM).

Keyword :

38-kDa adhesin protein, M. tuberculosis, lung, macrophage,


References :

  1. Price SA, Wilson LM, (2006). PATOFISOLOGI : Konsep Klinis Proses- Proses Penyakit. Ed 6. vol 1 Hal: 86-99 : EGC
  2. Bender BS, Rowe CA, Taylor SF, Wyatt LS, Moss B and Small PA, (2004). Single Intranasal Mucosal Mycobacterium bovis BCG Vaccination Confers Improved Protection Compared to Subcutaneous Vaccination against Pulmonary Tuberculosis. Hal 238-246 : Infection and Immunity


   


Archive Article

Cover Media Content

Volume : 21 / No. : 2 / Pub. : 2015-03
  1. Pneumatic tube on routine blood test and lactate dehydrogenase
  2. Detection in tetrazolium microplate assay culture methods from pulmonary tuberculosis suspected sputum
  3. High molecular weight adiponectin and vasculer thickness in diabetes type 2 related to fixed dose combination of glimepiride and metformin
  4. Neutrofil/lymphocyte ratio in carcinoma mammae
  5. Platelet aggregation and mean volume with metabolic syndrome in obesity
  6. Diagnosis of pulmonary tuberculosis based on frequency of sputum examination
  7. Genetic variation of hepatitis b virus polymerase gene from chronic hepatitis b infected patient with telbivudine therapy
  8. The 38 kda adhesin protein of mycobacterium tuberculosis and macrophage of the lung
  9. Bacterial pattern and patient’s age on procalcitonin in community and hospital acquired pneumonia
  10. Aspergillus glaucus group and penicillium sp in neurosurgery operating theater
  11. Diagnostic value of iga antivca epstein-barr antibody in nasophryngeal carcinoma
  12. Blood glucose test between hexokinase with glucose oxidase and glucose dehydrogenase methods in diabetes mellitus
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  14. Lipoprotein(a) in acute coronary syndrome
  15. D-dimer plasma levels in ishemic stroke
  16. Adrenomedullin’s in breast cancer with metastatic state
  17. Storage temperature for 24 hours of uric acid in urine
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